Identification of blast resistance genes in elite indica-type varieties of rice (Oryza sativa L.)

Seven blast resistance genes- Pi20, Pita, Pik (one of Pik alleles, Pik, Pik-h, Pik-m, and Pik-p), Pib, Pik-s, Piz-t, and Pia- were identified in International rice Research Institute (IRRI)- bred rice varieties through genetic analysed based on a differential system. Segregation analyses were performed using BCF populations derived from crosses of six varieties (IR34, IR36, IR60, IR74, IR46 and IR64) with a susceptible Indica-type variety C)39, and allelism tests using F populations derived from crossed of nine varieties (IR34, IR24, IR36, IR60, PSB Rcl, IR74, IR56, IR70, and IR64) with differential varieties carrying known blast resistance genes. Selected Philippine blast isolates of Pyricularia grisea (Cooke) Sacc. with known avirulence were used in the analyses. The genotype for blast resistance of each variety was identified based on a gene-for-gene relationship between resistance in rice and avirulence in the blast pathogen. Among the genes identified, Pib and Pik-s or Pik were detected in nearly all IRRI varieties used. Some genes that were not estimated from the reaction patterns of IRRI varieties in the previous analysis due to the masking effect of Pita, Pik, and Pi20 were also identified

Avirulence (AVR) gene-based diagnosis complements existing pathogen surveillance tools for effective deployment of resistance (R) genes against rice blast disease

Avirulence (AVR) genes in Magnaporthe oryzae, the fungal pathogen that causes the devastating rice blast disease, have been documented to be major targets subject to mutations to avoid recognition by resistance (R) genes. In this study, an AVR-gene-based diagnosis tool for determining the virulence spectrum of a rice blast pathogen population was developed and validated. A set of 77 single-spore field isolates was subjected to pathotype analysis using differential lines, each containing a single R gene, and classified into 20 virulent pathotypes, except for 4 isolates that lost pathogenicity. In all, 10 differential lines showed low frequency (95%), inferring the effectiveness of R genes present in the respective differential lines. In addition, the haplotypes of seven AVR genes were determined by polymerase chain reaction amplification and sequencing, if applicable. The calculated frequency of different AVR genes displayed significant variations in the population. AVRPiz-t and AVR-Pii were detected in 100 and 84.9% of the isolates, respectively. Five AVR genes such as AVR-Pik-D (20.5%) and AVR-Pik-E (1.4%), AVRPiz-t (2.7%), AVR-Pita (0%), AVR-Pia (0%), and AVR1-CO39 (0%) displayed low or even zero frequency. The frequency of AVR genes correlated almost perfectly with the resistance frequency of the cognate R genes in differential lines, except for International Rice Research Institute-bred blast-resistant lines IRBLzt-T, IRBLta-K1, and IRBLkp-K60. Both genetic analysis and molecular marker validation revealed an additional R gene, most likely Pi19 or its allele, in these three differential lines. This can explain the spuriously higher resistance frequency of each target R gene based on conventional pathotyping. This study demonstrates that AVR-gene-based diagnosis provides a precise, R-gene-specific, and differential line-free assessment method that can be used for determining the virulence spectrum of a rice blast pathogen population and for predicting the effectiveness of target R genes in rice varieties

Development of monogenic lines of rice for blast resistance

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Integrated strategies for durable rice blast resistance in sub-Saharan Africa

Rice is a key food security crop in Africa. The importance of rice has led to increasing country-specific, regional, and multinational efforts to develop germplasm and policy initiatives to boost production for a more food-secure continent. Currently, this critically important cereal crop is predominantly cultivated by small-scale farmers under suboptimal conditions in most parts of sub-Saharan Africa (SSA). Rice blast disease, caused by the fungus Magnaporthe oryzae, represents one of the major biotic constraints to rice production under small-scale farming systems of Africa, and developing durable disease resistance is therefore of critical importance. In this review, we provide an overview of the major advances by a multinational collaborative research effort to enhance sustainable rice production across SSA and how it is affected by advances in regional policy. As part of the multinational effort, we highlight the importance of joint international partnerships in tackling multiple crop production constraints through integrated research and outreach programs. More specifically, we highlight recent progress in establishing international networks for rice blast disease surveillance, farmer engagement, monitoring pathogen virulence spectra, and the establishment of regionally based blast resistance breeding programs. To develop blastresistant, high yielding rice varieties for Africa, we have established a breeding pipeline that utilizes real-Time data of pathogen diversity and virulence spectra, to identify major and minor blast resistance genes for introgression into locally adapted rice cultivars. In addition, the project has developed a package to support sustainable rice production through regular stakeholder engagement, training of agricultural extension officers, and establishment of plant clinics