Unveiling the power of microenvironment in liver regeneration: an in-depth overview

The liver serves as a vital regulatory hub for various physiological processes, including sugar, protein, and fat metabolism, coagulation regulation, immune system maintenance, hormone inactivation, urea metabolism, and water-electrolyte acid-base balance control. These functions rely on coordinated communication among different liver cell types, particularly within the liver’s fundamental hepatic lobular structure. In the early stages of liver development, diverse liver cells differentiate from stem cells in a carefully orchestrated manner. Despite its susceptibility to damage, the liver possesses a remarkable regenerative capacity, with the hepatic lobule serving as a secure environment for cell division and proliferation during liver regeneration. This regenerative process depends on a complex microenvironment, involving liver resident cells, circulating cells, secreted cytokines, extracellular matrix, and biological forces. While hepatocytes proliferate under varying injury conditions, their sources may vary. It is well-established that hepatocytes with regenerative potential are distributed throughout the hepatic lobules. However, a comprehensive spatiotemporal model of liver regeneration remains elusive, despite recent advancements in genomics, lineage tracing, and microscopic imaging. This review summarizes the spatial distribution of cell gene expression within the regenerative microenvironment and its impact on liver regeneration patterns. It offers valuable insights into understanding the complex process of liver regeneration

Litter size influences rumen microbiota and fermentation efficiency, thus determining host early growth in goats

IntroductionMultiple litters are accompanied by low birth weight, low survival rates, and growth rates in goats during early life. Regulating rumen microbiota structure can indirectly or directly affect host metabolism and animal growth. However, the relationship between high litter size and rumen microbiome, rumen fermentation, and growth performance in goat kids is unclear.MethodsIn the present study, thirty 6-month-old, female goats were investigated, of which 10 goats were randomly chosen from single, twin and triplet goats respectively, and their birth weight was recorded. From birth, all goats were subjected to the same feed and management practices. Individual weaning and youth body weight were measured, and the rumen fluid samples were collected to characterize the bacterial communities and to determine the ruminal volatile fatty acids (VFA), free amino acids (AA), and free fatty acids (FA) concentration of those young goats.Results and DiscussionCompared with the single and twin goats, triplet goats have lower weaning and youth body weight and average daily gain (ADG). Ruminal propionate, butyrate, and total VFA were decreased in triplet goats. Meanwhile, ruminal AA, such as branched chain amino acids (BCAA), essential amino acids (EAA), unsaturated fatty acids (UFA), and monounsaturated fatty acids (MUFA) were decreased, while saturated fatty acids (SFA) and odd and branched chain fatty acids (OBCFA) were increased in triplet goats. Our results also revealed that litter size significantly affected the rumen bacterial communities, and triplet goats had a lower the Firmicutes: Bacteroidota ratio, the abundance of Firmicutes phylum, Rikenellaceae family, and Rikenellaceae RC9 gut group, and had a higher proportion of Prevotellaceae family, and several genera of Prevotellaceae, such as Prevotella, and unclassified f Prevotellaceae. Furthermore, Spearman’s correlation network analysis showed that the changes in the rumen bacteria were associated with changes in rumen metabolites. In conclusion, this study revealed that high litter size could bring disturbances to the microbial communities and decrease the rumen fermentation efficiency and growth performance, which can be utilized to better understand variation in microbial ecology that will improve growth performance in triplet goats

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

A bandwidth-extended electrically small dipole antenna based on composite right/left handed (CRLH) transmission line

A bandwidth-extended electrically small dipole antenna based on composite right/left handed (CRLH) transmission line is presented. The proposed structure is realized by a periodic ladder network of four unit cells having air-gaps and wires.  Simulated results show that, adjusting the gaps and wires without any additional matching network, a 6.36% impedance bandwidth can be achieved when the length of the antenna is 50.4mm, and well-behaved radiation patterns have been displayed

Experimental Investigation on Metallic Droplet Behavior in Molten BOF slag

status: Published onlin

Research Progress on Purification Process Optimization and Content Determination of Zeaxanthin

At present, the purification process of zeaxanthin mainly includes organic solvent extraction, ultrasonic-assisted extraction and enzymeextraction, and the content determination technology mainly includes ultraviolet-spectrophotometry and high performance liquid chromatography. In this paper, the purification process and content determination technology of zeaxanthin in recent years are reviewed in order to provide ideas and theoretical basis for further research and application of zeaxanthin

Anti-inflammatory, Bacteriostatic and Anticancer Effects of Madecassoside

Madecassoside is a natural active component extracted from Centella asiatica. In recent years, a large number of studies have reported that madecassoside has a variety of biological activities, such as anticancer, anti-inflammatory and antibacterial effects, prevention and treatment of cardiovascular diseases, nerve damage, visceral damage and arthritis, and other pharmacological effects. In this paper, the pharmacological action and mechanism of madecassoside were reviewed to provide a theoretical basis for further research of madecassoside and drug development

Dynamic Changes in Carbon Sequestration from Opencast Mining Activities and Land Reclamation in China’s Loess Plateau

Opencast coal mining causes serious damage to the natural landscape, resulting in the depletion of the carbon sequestration capacity in the mining activity. There are few studies on the variation of carbon sequestration capabilities caused by land use changes in opencast mining areas. This paper uses six images were used to quantify the changes in land use types from 1986 to 2015 in the Pingshuo mining area in northwest China. At the same time, used statistical analysis and mathematical models to study soil and vegetation carbon sequestration. Results indicate that the total carbon sequestration exhibits a significant downward trend from 4.58 × 106 Mg in 1986 to 3.78 × 106 Mg in 2015, with the decrease of soil carbon sequestration accounting for the largest proportion. The carbon sequestration of arable land accounted for 51% of the total carbon sequestration in the mining area, followed by grassland (31%) and forestland (18%). Land reclamation contributed to the greatest increase in carbon sequestration of arable land from 17,890.15 Mg (1986) to 27,837.95 Mg (2015). Additionally, the downward trend in the carbon sequestration capacity of the mining ecosystem was mitigated after 2010 as the positive effects of land reclamation gradually amplified over time and as the mining techniques were greatly optimized in recent years in the Pingshuo mining area. Thus, terrestrial carbon sequestration can be improved through land reclamation projects and optimized mining activities. These results can help guide the utilization of reclaimed land in the future