Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice

The study was aimed to explore the possible function of thermal ablation treatment on T helper 17 (Th17) cells and regulatory T (Treg) cells in transplantation of hepatocellular carcinoma in mice. In total, 60 male C57BL/6 mice were divided into control group, model group, and treat group. Flow cytometry was used to detect the frequency of Th17 and Treg cells in peripheral blood. The levels of interleukin (IL)-17, IL-23, IL-10, and transforming growth factor beta (TGF-β) in serum were detected by enzyme-linked immunosorbent assay (ELISA).The levels of IL-17, RORγt, Foxp3, and TGF-β mRNA in tumor tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR). Compared with the model group, tumor size was significantly decreased after thermal ablation treatment. After treatment, the frequency of Th17 cells in peripheral blood was significantly decreased, while the frequency of Treg cells was profoundly increased ( P  < 0.05). The levels of IL-17 and IL-23 were significantly downregulated, while IL-10 and TGF-β levels were upregulated ( P  < 0.05). IL-17 and RORγt mRNA levels in tumor tissues were significantly decreased ( P  < 0.05), and Foxp3 and TGF-β mRNA levels were significantly increased ( P  < 0.05). Thermal ablation treatment plays a positive role in the treatment of hepatoma in mice through affecting the imbalance of Th17/Treg cells

Plant and soil fertility characteristics of different winter wheat fields in the Huan-huai-hai plain of China

High-profile nutrient accumulation has caused low nutrient use efficiency and environmental pollution. A total of 140 points in farmers’ wheat fields were investigated and sampled under a typical winter wheat and summer maize rotation system in the Huang-huai-hai Plain of China (HPC). The average wheat yield in the high-yield field is more than 10% relative to the mid-low-yield areas based on historical data. The results showed in the high-yield wheat field the effective spike number was the main yield component, followed by 1000-grain weight. Uptake contents and ratios of N, P2O5 and K2O in the whole wheat plant were also examined. Along the soil depth, organic matter (OM), alkali-hydrolyzable N (AN), available P (AP) and available K (AK) concentration gradually decreased. Soil critical values of AN, AP and AK in the 0–20 cm depth needed to achieve the maximum grain yield were 85.9, 17.7 and 114.2 mg kg−1 calculated by the significant linear-plus-plateau model between wheat grain yield and soil nutrient concentration at harvest, respectively. These results indicated that the rational application of fertilizer based on the soil nutrient supply capacity and residual nutrients in root zone and optimum planting density are key determinants of grain production in the HPC