Divergent Mating Behaviors and the Evolution of Reproductive Isolation

Sexual selection can cause rapid co-divergence of mating traits and mate preferences, generate reproductive barriers among individuals bearing divergent mating traits, and potentially lead to speciation. In my dissertation, I focused on two emerging topics that challenge this traditional speciation-by-mate-choice paradigm. First, sexual selection encompasses both mate preferences and intrasexual competition, yet speciation research disproportionally focused on the role of the former. Second, sexual behaviors are usually assumed to be genetically inherited, but they may often be shaped by learning instead, which can generate very different evolutionary trajectories for traits and preferences. Using studies of the highly polymorphic strawberry poison frogs (Oophaga pumilio), I demonstrated how incorporating (i) male-male competition and (ii) behavioral learning can enhance our understanding of the potential for speciation to be driven by sexual selection. I first characterized behavioral patterns across a natural contact zone between color morphs and showed that coloration (the divergent mating trait) mediates both female choice and male-male competition. Females often prefer males of their own (local) color over a novel color, and males, when defending territories, are more aggressive against their own color morph. I then tested how these color-mediated female preferences and male aggression biases interact to determine mating patterns. I conducted a controlled breeding experiment in which male-male competition and female mate choice act either in same or in opposing directions. In this study, females reproduced more often with the territorial male over the non-territorial male, regardless of the males’ coloration. This challenges the common assumption that knowledge of female preferences for male mating traits is sufficient to predict mating patterns. Finally, I discovered that learning from mothers during the tadpole stage shapes both female mate preferences and male aggression biases in O. pumilio. Based on this finding, I built a population genetic model and used it to demonstrate a simple and elegant mechanism by which sexual selection alone has the potential to initiate speciation. My research highlights the importance of considering interactions between mate choice, intrasexual competition, and behavioral learning, for studies of mating trait evolution and sexual selection’s role in speciation

Pilots’ visual scan pattern and situation awareness in flight operations

Introduction: Situation awareness (SA) is considered an essential prerequisite for safe flying. If the impact of visual scanning patterns on a pilot’s situation awareness could be identified in flight operations, then eye-tracking tools could be integrated with flight simulators to improve training efficiency. Method: Participating in this research were 18 qualified, mission-ready fighter pilots. The equipment included high-fidelity and fixed-base type flight simulators and mobile head-mounted eye-tracking devices to record a subject’s eye movements and SA while performing air-to-surface tasks. Results: There were significant differences in pilots’ percentage of fixation in three operating phases: preparation (M = 46.09, SD = 14.79), aiming (M = 24.24, SD = 11.03), and release and break-away (M = 33.98, SD = 14.46). Also, there were significant differences in pilots’ pupil sizes, which were largest in the aiming phase (M = 27,621, SD = 6390.8), followed by release and break-away (M = 27,173, SD = 5830.46), then preparation (M = 25,710, SD = 6078.79), which was the smallest. Furthermore, pilots with better SA performance showed lower perceived workload (M = 30.60, SD = 17.86), and pilots with poor SA performance showed higher perceived workload (M = 60.77, SD = 12.72). Pilots’ percentage of fixation and average fixation duration among five different areas of interest showed significant differences as well. Discussion: Eye-tracking devices can aid in capturing pilots’ visual scan patterns and SA performance, unlike traditional flight simulators. Therefore, integrating eye-tracking devices into the simulator may be a useful method for promoting SA training in flight operations, and can provide in-depth understanding of the mechanism of visual scan patterns and information processing to improve training effectiveness in aviation

Pilots’ visual scan pattern and attention distribution during the pursuit of a dynamic target

Introduction: The current research is investigating pilots’ visual scan patterns in order to assess attention distribution during air-to-air manoeuvers. Method: A total of thirty qualified mission-ready fighter pilots participated in this research. Eye movement data were collected by a portable head-mounted eye-tracking device, combined with a jet fighter simulator. To complete the task, pilots have to search for, pursue, and lock-on a moving target whilst performing air-to-air tasks. Results: There were significant differences in pilots’ saccade duration (msec) in three operating phases including searching (M=241, SD=332), pursuing (M=311, SD=392), and lock-on (M=191, SD=226). Also, there were significant differences in pilots’ pupil sizes (pixel2) of which lock-on phase was the largest (M=27237, SD=6457), followed by pursuing (M=26232, SD=6070), then searching (M=25858, SD=6137). Furthermore, there were significant differences between expert and novice pilots on the percentage of fixation on the HUD, time spent looking outside the cockpit, and the performance of situational awareness (SA). Discussion: Experienced pilots have better SA performance and paid more attention to the HUD but focused less outside the cockpit when compared with novice pilots. Furthermore, pilots with better SA performance exhibited a smaller pupil size during the operational phase of lock-on whilst pursuing a dynamic target. Understanding pilots’ visual scan patterns and attention distribution are beneficial to the design of interface displays in the cockpit and in developing human factors training syllabi to improve safety of flight operations

Constructing Cooking Ontology for Live Streams

We build a cooking domain knowledge by using an ontology schema that reflects natural language processing and enhances ontology instances with semantic query. Our research helps audiences to better understand live streaming, especially when they just switch to a show. The practical contribution of our research is to use cooking ontology, so we may map clips of cooking live stream video and instructions of recipes. The architecture of our study presents three sections: ontology construction, ontology enhancement, and mapping cooking video to cooking ontology. Also, our preliminary evaluations consist of three hierarchies—nodes, ordered-pairs, and 3-tuples—that we use to referee (1) ontology enhancement performance for our first experiment evaluation and (2) the accuracy ratio of mapping between video clips and cooking ontology for our second experiment evaluation. Our results indicate that ontology enhancement is effective and heightens accuracy ratios on matching pairs with cooking ontology and video clips

Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus.

The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2'-O-(1,2-diacyl-sn-glycero-3-phospho) -3'-O-(alpha-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(alpha-N-acetylglucosaminyl)-N-glyceroyl alkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-O-phosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C(15:0), -C(16:0), and -C(17:0) and anteiso-C(15:0) and -C(17:0). The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063. Accordingly, the ratios of iso- to anteiso-branched fatty acids increase when grown at the higher temperature

Granulocyte Colony-Stimulating Factor Alleviates Bacterial-Induced Neuronal Apoptotic Damage in the Neonatal Rat Brain through Epigenetic Histone Modification

Bacterial meningitis during the perinatal period may cause long-term neurological deficits. The study investigated whether bacterial lipopolysaccharide (LPS) derived from E. coli. led to neuronal apoptosis with an impaired performance of long-term cognitive function involving the activation of histone modification in the TNF-α gene promoter. Further, we looked into the therapeutic efficacy of granulocyte colony-stimulating factor (G-CSF) in a neonatal brain suffering from perinatal bacterial meningitis. We applied the following research techniques: neurobehavioral tasks, confocal laser microscopy, chromatin immunoprecipitation, and Western blotting. At postnatal day 10, the animals were subjected to LPS and/or G-CSF. The target brain tissues were then collected at P17. Some animals (P45) were studied using neurobehavioral tasks. The LPS-injected group revealed significantly increased expression of NF-κB phosphorylation and trimethylated H3K4 in the TNFA gene promoter locus. Furthermore, the caspase-3, neuronal apoptosis expression, and an impaired performance in cognitive functions were also found in our study. Such deleterious outcomes described above were markedly alleviated by G-CSF therapy. This study suggests that selective therapeutic action sites of G-CSF through epigenetic regulation in the TNFA gene promoter locus may exert a potentially beneficial role for the neonatal brain suffering from perinatal bacterial-induced meningitis

Spectral filtering of multiple directly modulated channels for WDM access networks by using an FP etalon

[[abstract]]Spectral and waveform reshaping schemes can enhance the transmission distance of fiber links that use directly modulated lasers as transmitters. We prove the feasibility of using a simple Fabry–Perot (FP) etalon as the spectral reshaper for applications in wavelength division multiplexing (WDM) access networks. The transient chirp and adiabatic chirp of a directly modulated laser are analyzed in detail by using the time-resolved chirp measurement. The effects of the original extinction ratio and the adiabatic chirp on the spectral reshaping are clarified to obtain the optimal operation conditions. It is shown that placing a single-cavity FP etalon filter after multiple 10 Gbits/s directly modulated lasers can extend their transmission distances from 10 to 50 km in the 1.55 m wavelength window. Due to the limited filtering capability of the etalon, the choice of the original extinction ratio and finesse of the etalon is discussed in detail from the experiments and simulation.[[incitationindex]]SCI[[incitationindex]]EI[[booktype]]紙本[[booktype]]電子

Yeast Ataxin-2 Forms an Intracellular Condensate Required for the Inhibition of TORC1 Signaling during Respiratory Growth

Yeast ataxin-2, also known as Pbp1 (polyA binding protein-binding protein 1), is an intrinsically disordered protein implicated in stress granule formation, RNA biology, and neurodegenerative disease. To understand the endogenous function of this protein, we identify Pbp1 as a dedicated regulator of TORC1 signaling and autophagy under conditions that require mitochondrial respiration. Pbp1 binds to TORC1 specifically during respiratory growth, but utilizes an additional methionine-rich, low complexity (LC) region to inhibit TORC1. This LC region causes phase separation, forms reversible fibrils, and enables self-association into assemblies required for TORC1 inhibition. Mutants that weaken phase separation in vitro exhibit reduced capacity to inhibit TORC1 and induce autophagy. Loss of Pbp1 leads to mitochondrial dysfunction and reduced fitness during nutritional stress. Thus, Pbp1 forms a condensate in response to respiratory status to regulate TORC1 signaling