Pengetahuan, kemahiran dan amalan guru membina item kemahiran berfikir aras tinggi (KBAT) dalam instrumen pentaksiran pembelajaran

Kajian ini dijalankan bagi mengenal pasti tahap pengetahuan, kemahiran dan amalan guru membina item kemahiran berfikir aras tinggi (KBAT) dalam instrumen pentaksiran pembelajaran. Kajian ini juga melihat perbezaan tahap pengetahuan, kemahiran dan amalan guru membina item KBAT berdasarkan kepada kumpulan guru mengajar matapelajaran tingkatan 3 dan kumpulan guru mengajar matapelajaran tingkatan 5. Sebanyak enam persoalan kajian telah dibangunkan bagi mengkaji permasalahan kajian. Rekabentuk kajian ini melibatkan analisis deskriptif dan inferensi dalam bentuk tinjauan yang melibatkan data kuantitatif dengan menggunakan borang soal selidik berskala likert lima mata sebagai instrumen kajian. Seramai 161 sampel guru daripada 3 buah sekolah di daerah Muar, Johor terpilih menjadi responden dalam kajian ini. Model Pengukuran Rasch telah digunakan bagi menentukan kesahan dan kebolehpercayaan instrumen kajian yang telah dibina sendiri. Hasil analisis kajian menunjukkan tahap pengetahuan, kemahiran dan amalan guru membina item KBAT adalah berada pada tahap tinggi. Dapatan kajian juga menunjukkan tidak terdapat perbezaan yang signifikan di antara kumpulan guru mengajar matapelajaran tingkatan 3 dan kumpulan guru mengajar matapelajaran tingkatan 5 terhadap tahap pengetahuan, kemahiran dan amalan membina item KBAT. Hasil kajian ini dapat dijadikan sebagai satu garis panduan kepada guru-guru yang mengubal item pentaksiran dan program peningkatan profesionalisme guru di sekolah. Kajian lanjutan juga boleh dilaksanakan bagi memperbaiki kekurangan dalam kajian ini

The Genetic Mechanism of Inertinite in the Middle Jurassic Inertinite-Rich Coal Seams of the Southern Ordos Basin

Inertinite is an important type of organic maceral in coal deposits, andalso an important geological information carrier of coal forming environments. In the southern section of the Ordos Basin, the No. 4 inertinite-richcoal seam of the Middle Jurassic Yan’an Formation in the Binchang Coalfield was selected as an example to study the genetic mechanism of theinertinite. In this study, the results obtained from experimental tests ofcoal rock, including principal and trace elements, stable carbon isotopes,scanning electron microscopy, inertinite reflectance, sporopollen andfree radical retorting methods, were analyzed. Then, the findings werecombined with the previous understanding of the oxygen content in theatmosphere and ground fire characteristics, in order to discuss the genesismechanism of inertinite in the No. 4 coal seam. The obtained researchresults were as follows: (1) During the coal forming period of the No. 4coal seam, the overall climate had been relatively dry. There were fourrelatively dry-wet climate cycles in the No.4 coal seam, which werecontrolled by the eccentricity astronomical period. The inertinite contentwere relatively high during the dry periods; (2) The temperature rangesuitable for microorganism activities during the oxidation processes wasbetween 0 and 80℃ . The simulation results of the free radical concentrations showed that the maximum temperature of fusain in the No. 4 coalseam during the process of coalification had not exceeded 300℃ , whichwas significantly higher than the temperature range of microorganismactivities. Therefore, these were not conducive to the activities of microorganism and formation of inertinite during the coal-forming period;(3) The genesis temperature of the inertinite in the No. 4 coal seam wascalculated according to the reflectance of the inertinite, which was lowerthan 400 ℃ . This result supported the cause of wildfire of the inertiniteand reflected that the type of wildfire was mainly ground fire, along withpartially surface fire. Moreover, the paleogeographic location, climaticconditions, atmospheric oxygen concentration, etc. of the study areashowed that the conditions for wildfire events were in fact available; (4)There were dense and scattered fusinite observed in the No. 4 coal seam,and the thickness of cell walls were found to differ. It was speculated thatthis was related to the type of wildfire, combustion temperatures, combustion timeframes, and different initial conditions of the burned objectsduring the coal forming periods

p21-activated kinase is involved in the sporulation, pathogenicity, and stress response of Arthrobotrys oligospora under the indirect regulation of Rho GTPase-activating protein

The p21-GTPase-activated protein kinases (PAKs) participate in signal transduction downstream of Rho GTPases, which are regulated by Rho GTPase-activating proteins (Rho-GAP). Herein, we characterized two orthologous Rho-GAPs (AoRga1 and AoRga2) and two PAKs (AoPak1 and AoPak2) through bioinformatics analysis and reverse genetics in Arthrobotrys oligospora, a typical nematode-trapping (NT) fungus. The transcription analyses performed at different development stages suggested that Aopaks and Aorga1 play a crucial role during sporulation and trap formation, respectively. In addition, we successfully deleted Aopak1 and Aorga1 via the homologous recombination method. The disruption of Aopak1 and Aorga1 caused a remarkable reduction in spore yield and the number of nuclei per cell, but did not affect mycelial growth. In ∆Aopak1 mutants, the trap number was decreased at 48 h after the introduction of nematodes, but nematode predatory efficiency was not affected because the extracellular proteolytic activity was increased. On the contrary, the number of traps in ∆Aorga1 mutants was significantly increased at 36 h and 48 h. In addition, Aopak1 and Aorga1 had different effects on the sensitivity to cell-wall-disturbing reagent and oxidant. A yeast two-hybrid assay revealed that AoPak1 and AoRga1 both interacted with AoRac, and AoPak1 also interacted with AoCdc42. Furthermore, the Aopaks were up-regulated in ∆Aorga1 mutants, and Aorga1 was down-regulated in ∆Aopak1 mutants. These results reveal that AoRga1 indirectly regulated AoPAKs by regulating small GTPases

Autoantibodies against the Catalytic Domain of BRAF Are Not Specific Serum Markers for Rheumatoid Arthritis

BACKGROUND: Autoantibodies to the catalytic domain of v-raf murine sarcoma viral oncogene homologue B1 (BRAF) have been recently identified as a new family of autoantibodies involved in rheumatoid arthritis (RA). The objective of this study was to determine antibody responses to the catalytic domain of BRAF in RA and other autoimmune diseases. The association between RA-related clinical indices and these antibodies was also assessed. METHODOLOGY/PRINCIPAL FINDINGS: The presence of autoantibodies to the catalytic domain of BRAF (anti-BRAF) or to peptide P25 (amino acids 656-675 of the catalytic domain of BRAF; anti-P25) was determined in serum samples from patients with RA, primary Sjögren's syndrome (pSS), systemic lupus erythematosus (SLE), and healthy controls by using indirect enzyme-linked immunosorbent assays (ELISAs) based on the recombinant catalytic domain of BRAF or a synthesized peptide, respectively. Associations of anti-BRAF or anti-P25 with disease variables of RA patients were also evaluated. Our results show that the BRAF-specific antibodies anti-BRAF and anti-P25 are equally present in RA, pSS, and SLE patients. However, the erythrocyte sedimentation rate (ESR) used to detect inflammation was significantly different between patients with and without BRAF-specific antibodies. The anti-BRAF-positive patients were found to have prolonged disease, and active disease occurred more frequently in anti-P25-positive patients than in anti-P25-negative patients. A weak but significant correlation between anti-P25 levels and ESRs was observed (r = 0.319, p = 0.004). CONCLUSIONS/SIGNIFICANCE: The antibody response against the catalytic domain of BRAF is not specific for RA, but the higher titers of BRAF-specific antibodies may be associated with increased inflammation in RA

Two-path network with feedback connections for pan-sharpening in remote sensing

High-resolution multi-spectral images are desired for applications in remote sensing. However, multi-spectral images can only be provided in low resolutions by optical remote sensing satellites. The technique of pan-sharpening wants to generate high-resolution multi-spectral (MS) images based on a panchromatic (PAN) image and the low-resolution counterpart. The conventional deep learning based pan-sharpening methods process the panchromatic and the low-resolution image in a feedforward manner where shallow layers fail to access useful information from deep layers. To make full use of the powerful deep features that have strong representation ability, we propose a two-path network with feedback connections, through which the deep features can be rerouted for refining the shallow features in a feedback manner. Specifically, we leverage the structure of a recurrent neural network to pass the feedback information. Besides, a power feature extraction block with multiple projection pairs is designed to handle the feedback information and to produce power deep features. Extensive experimental results show the effectiveness of our proposed method

Meteorin-like/Metrnl, a novel secreted protein implicated in inflammation, immunology, and metabolism: A comprehensive review of preclinical and clinical studies

Meteorin-like, also known as Metrnl, Meteorin-β, Subfatin, and Cometin, is a novel secreted protein exerting pleiotropic effects on inflammation, immunology, and metabolism. Earlier research on this hormone focused on regulating energy expenditure and glucose homeostasis. Consequently, several studies attempted to characterize the molecule mechanism of Metrnl in glucose metabolism and obesity-related disorders but reported contradictory clinical results. Recent studies gradually noticed its multiple protective functions in inflammatory immune regulations and cardiometabolic diseases, such as inducing macrophage activation, angiogenesis, tissue remodeling, bone formation, and preventing dyslipidemias. A comprehensive understanding of this novel protein is essential to identify its significance as a potential therapeutic drug or a biomarker of certain diseases. In this review, we present the current knowledge on the physiology of Metrnl and its roles in inflammation, immunology, and metabolism, including animal/cell interventional preclinical studies and human clinical studies. We also describe controversies regarding the data of circulation Metrnl in different disease states to determine its clinical application better

Prolonged dual antiplatelet therapy in patients with non-ST-segment elevation myocardial infarction: 2-year findings from EPICOR Asia.

BACKGROUND: Patients with non-ST-segment elevation myocardial infarction (NSTEMI) have a generally poor prognosis and antithrombotic management patterns (AMPs) used post-acute coronary syndrome (ACS) remain unclear. Duration of dual antiplatelet therapy (DAPT) and patient characteristics was evaluated in NSTEMI patients enrolled in EPICOR Asia. HYPOTHESIS: Patients stopping DAPT early may benefit from more intensive monitoring. METHODS: EPICOR Asia was a prospective, real-world, primary data collection, cohort study in adults with an ACS, conducted in eight countries/regions in Asia, with 2 year follow-up. Eligible patients were hospitalized within 48 hours of symptom onset and survived to discharge. We describe AMPs and baseline characteristics in NSTEMI patients surviving ≥12 months with DAPT duration ≤12 and > 12 months post-discharge. Clinical outcomes (composite of death, myocardial infarction, and stroke; and bleeding) were also explored. RESULTS: At discharge, 90.8% of patients were on DAPT (including clopidogrel, 99%). At 1- and 2-year follow-up, this was 79.2% and 60.0%. Patients who stopped DAPT ≤12 months post-discharge tended to be older, female, less obese, have prior cardiovascular disease, and have renal dysfunction. While causality cannot be inferred, the incidence of the composite endpoint over the subsequent 12 months was 10.6% and 3.1% with shorter vs longer use of DAPT, and mortality risk over the same period was 8.4% and 1.6%. CONCLUSIONS: Over 90% of NSTEMI patients were discharged on DAPT, with 60% on DAPT at 2 years. Patients stopping DAPT early were more likely to have higher baseline risk and may therefore benefit from more intensive monitoring during long-term follow-up