Amn1 governs post-mitotic cell separation in Saccharomyces cerevisiae

<div><p>Post-mitotic cell separation is one of the most prominent events in the life cycle of eukaryotic cells, but the molecular underpinning of this fundamental biological process is far from being concluded and fully characterized. We use budding yeast <i>Saccharomyces cerevisiae</i> as a model and demonstrate <i>AMN1</i> as a major gene underlying post-mitotic cell separation in a natural yeast strain, YL1C. Specifically, we define a novel 11-residue domain by which Amn1 binds to Ace2. Moreover, we demonstrate that Amn1 induces proteolysis of Ace2 through the ubiquitin proteasome system and in turn, down-regulates Ace2’s downstream target genes involved in hydrolysis of the primary septum, thus leading to inhibition of cell separation and clumping of haploid yeast cells. Using ChIP assays and site-specific mutation experiments, we show that Ste12 and the a1-α12 heterodimer are two direct regulators of <i>AMN1</i>. Specifically, a1-α2, a diploid-specific heterodimer, prevents Ste12 from inactivating <i>AMN1</i> through binding to its promoter. This demonstrates how the Amn1-governed cell separation is highly cell type dependent. Finally, we show that <i>AMN1</i>^<i>368D</i> from YL1C is a dominant allele in most strains of <i>S</i>. <i>cerevisiae</i> and evolutionarily conserved in both genic structure and phenotypic effect in two closely related yeast species, <i>K</i>. <i>lactis</i> and <i>C</i>. <i>glabrata</i>.</p></div

Unconscious and conscious acceptance downregulate aggressive behavior: Mediating role of anger regulation

Social exclusion can induce negative emotions and aggression. While previous studies have investigated the effect of trait acceptance on emotional experience and aggression during social exclusion, it is still unclear how different forms of acceptance strategy can downregulate negative emotions and whether this potential reduction of negative emotions should mediate the effect of acceptance on aggression. To address these questions, 100 participants were recruited and randomly divided into three groups: control group (CG, N = 33), conscious acceptance group (CAG, N = 33) and unconscious acceptance group (UAG, N = 34). Negative emotions were induced by the cyberball game and measured by the modified PANAS. Aggressive behavior was assessed by the hot sauce allocation task. Results showed that anger, rather than other negative emotions, mediated the effect of acceptance on aggressive behavior. Conscious and unconscious acceptance both effectively regulated anger, hurt feelings and aggressive behavior during social exclusion. Compared to conscious acceptance, unconscious acceptance was associated with less reduction of positive emotion and had a better effect on reducing sadness. These findings highlight the advantage of applying unconscious acceptance strategy to regulating social exclusion-induced emotions for the purpose of reducing aggressive behavior

Trait Acceptance Buffers Aggressive Tendency by the Regulation of Anger during Social Exclusion

Social exclusion has led to increased negative emotions and aggressive behaviors, two outcomes that are correlated with each other. Thus, the down-regulation of negative emotions appears to play a crucial role in reducing the tendency for aggressive behavior. However, this assumption has not yet been tested. To this end, a total of 397 undergraduates reported their aggressive tendencies, state emotions and trait acceptance by completing corresponding questionnaires, and a recall paradigm was used to induce experiences of social exclusion. The results showed that in the context of social exclusion, (1) trait acceptance was negatively correlated with negative emotions and aggressive tendency but was positively correlated with positive emotions; (2) negative emotions, rather than positive emotions, were positively correlated with aggressive tendency; (3) increased trait acceptance buffered the experience of anger, which is, in turn, related to reduced aggressive tendency; (4) trait acceptance also downregulated the feeling of sadness, which is, however, related to increased aggression; (5) the mediator of sadness was smaller in effect size than that of anger. Taken together, these results suggest that negative emotions are associated with aggression in the context of social exclusion, and the habitual use of an acceptance strategy was conductive to decreasing aggressive tendencies by decreasing anger

Research on the revitalization of the cultural tourism industry in the post-epidemic era——a case study of Guangxi

As a sudden public health event, the COVID-19 epidemic has brought a tremendous impact on the economic and social development of China. Its biggest and most direct impact is in the tertiary industry, especially the cultural tourism industry, which has a more far-reaching impact. This paper takes Guangxi as an example to study the current situation, problems, and future development direction of the cultural tourism industry in the post-epidemic era. The study found that the response to the epidemic in ethnic minority areas represented by Guangxi was timely and rapid, but due to their industrial resilience and the impact of the epidemic, the revitalization of the culture and tourism industry is still facing severe challenges. Therefore, this article put forward the key countermeasure to promote the cultural tourism industry revitalization of ethnic minority areas from list management, elements of development management, project construction, industry integration, and international cooperation, to provide policy recommendations and theoretical basis for the revitalization of the cultural tourism industry in ethnic minority areas

Rational Design of Lewis Base Electron Transport Materials for Improved Interface Property in Inverted Perovskite Solar Cells: A Theoretical Investigation

Electron transport materials (ETMs) play a vital role in electron extraction and transport at the perovskite/ETM interface of inverted perovskite solar cells (PSCs) and are useful in power conversion efficiency (PCE), which is limited by interface carrier recombination. However, strategies for passivating undercoordinated Pb2+ at the perovskite/ETM interface employing ETMs remain a challenge. In this work, a variety of heteroatoms were used to strengthen the Lewis base property of new ETMs (asymmetrical perylene-diimide), aimed at deactivating non-bonded Pb2+ at the perovskite surface through Lewis acid-base coordination. Quantum chemical analysis revealed that novel ETMs have matched the energy level of perovskite, which enables electron extraction at the perovskite/ETM interface. The results also suggest that the large electron mobility (0.57~5.94 cm2 V−1 s−1) of designed ETMs shows excellent electron transporting ability. More importantly, reinforced interaction between new ETMs and Pb2+ was found, which is facilitating to passivation of the defects induced by unsaturated Pb2+ at the perovskite/ETM interface. Furthermore, it is found that MA (CH3NH3+), Pb, and IPb (iodine substituted on the Pb site) defects at the perovskite/ETM interface could be effectively deactivated by the new ETMs. This study provides a useful strategy to design ETMs for improving the interface property in PSCs

Time-Dependent Afterglow from a Single Component Organic Luminogen

Pure organic luminogens with long-persistent luminescence have been extensively studied, on account of their fundamental research significance and diverse utilizations in anticounterfeiting, bioimaging, encryption, organic light-emitting diodes, chemo-sensing, etc. However, time-dependent color-tunable afterglow is rarely reported, especially for single-component materials. In this work, we reported an organic luminogen with time-dependent afterglow, namely, benzoyleneurea (BEU), with multiple persistent room-temperature phosphorescence (p-RTP) and thermally activated delayed fluorescence (TADF) in single crystals. While the lifetime of TADF is relatively short (~1.2 ms), those for p-RTP are as long as around 369~754 ms. The comparable but different decay rates of diversified p-RTP emissions endow BEU crystals with obvious time-dependent afterglow. The existence of multiple emissions can be reasonably illustrated by the clustering-triggered emission (CTE) mechanism. Single-crystal structure illustrates that the combination of benzene ring and nonconventional chromophores of ureide helps facilitate divergent intermolecular interactions, which contribute to the formation of varying emissive species. Moreover, its methyl- and chloro-substituted derivatives show similar multiple p-RTP emissions. However, no time-dependent afterglows are observed in their crystals, due to the highly approaching lifetimes. The afterglow color variation of BEU crystals grants its applications in advanced anticounterfeiting field and information encryption

Cleaner production of baicalein by novel high-temperature-little-acid hydrolysis of baicalin

ABSTRACTBaicalein, usually obtained after the removal of 7-O-β-D-glucuronic acid from baicalin, has strong pharmacological activity. In this study, a novel strategy for efficient and eco-friendly preparation of baicalein was established by high-temperature-little-acid hydrolysis of baicalin. The effects of inorganic acids, solvents, solid–liquid ratio, HCl concentration and hydrolysis duration on the yield of baicalein were investigated by one-factor-at-a-time experiment. Then, an orthogonal experiment design was conducted for the optimal hydrolysis conditions, namely, baicalin was boiled in n-BuOH solution containing 0.50% HCl (w/v) at 130°C for 2.0 h with solid–liquid ratio at 1:25 (g/mL). In the 60-fold scale-up experiment, the yield of baicalein was achieved at 83.5% ± 6.3%, and its purity was increased from 74.2% to 98.7% after purification. Compared with reported methods, acid consumption per unit product of the newly developed strategy was approximately 80 times lower than that of conventional acid hydrolysis, and time consumption was substantially reduced. Overall, the novel strategy showed great prospects for a cleaner large-scale production of baicalein, which provides a new alternative desirable for the preparation of natural products with high efficiency and low consumption in the pharmaceutical industry

Sampling variation of RAD-seq data from diploid and tetraploid potato (Solanum tuberosum L.)

The new sequencing technology enables identification of genome-wide sequence-based variants at a population level and a competitively low cost. The sequence variant-based molecular markers have motivated enormous interest in population and quantitative genetic analyses. Generation of the sequence data involves a sophisticated experimental process embedded with rich non-biological variation. Statistically, the sequencing process indeed involves sampling DNA fragments from an individual sequence. Adequate knowledge of sampling variation of the sequence data generation is one of the key statistical properties for any downstream analysis of the data and for implementing statistically appropriate methods. This paper reports a thorough investigation on modeling the sampling variation of the sequence data from the optimized RAD-seq (Restriction sit associated DNA sequencing) experiments with two parents and their offspring of diploid and autotetraploid potato (Solanum tuberosum L.). The analysis shows significant dispersion in sampling variation of the sequence data over that expected under multinomial distribution as widely assumed in the literature and provides statistical methods for modeling the variation and calculating the model parameters, which may be easily implemented in real sequence datasets. The optimized design of RAD-seq experiments enabled effective control of presentation of undesirable chloroplast DNA and RNA genes in the sequence data generated

Study on the Coupling Effect of a Solar-Coal Unit Thermodynamic System with Carbon Capture

Based on the structural theory of thermo-economics, a 600 MW unit was taken as an example. An integration system which uses fuel gas heat and solar energy as a heat source for post-combustion carbon capture was proposed. The physical structure sketch and productive structure sketch were drawn and a thermo-economics model and cost model based on the definition of fuel-product were established. The production relation between units was analyzed, and the composition and distribution of the exergy cost and thermo-economic cost of each unit were studied. Additionally, the influence of the fuel price and equipment investment cost of the thermo-economic cost for each product was studied. The results showed that the main factors affecting the unit cost are the fuel exergy cost, component exergy efficiency, and irreversible exergy cost of each unit, and the main factors affecting the thermo-economics cost are the specific irreversible exergy cost and investment exergy cost. The main factors affecting the thermal economics of solar energy collectors and low-pressure economizers are the invested exergy cost, negentropy exergy cost, and irreversible exergy cost of each unit