1,448 research outputs found
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Carbon dioxide dynamics from sediment, sediment-water interface and overlying water in the aquaculture shrimp ponds in subtropical estuaries, southeast China
Aquaculture ponds can emit a large amount carbon dioxide (CO2), with the consequence of exacerbating global climate change. Many studies about CO2 dynamics across the water-air interface, but CO2 in sediment and overlying water received relative less attention. In this study, CO2 concentration in sediment porewater, the diffusive CO2 fluxes across the sediment-water interface (SWI), and the CO2 production rates in the overlying water (CO2_WP) were determined in the shrimp ponds in the Min River Estuary (MRE) and Jiulong River Estuary (JRE), southeast China, to analyze the dynamics of CO2 among different growth stages of shrimps. Our results showed large variations in porewater CO2 concentrations, CO2 diffusive fluxes and CO2_WP rates among different growth stages, with markedly larger values in the middle stage of shrimp growth. The temporal variation of CO2 in both estuarine ponds followed closely the seasonal change of temperature. The internal CO2 production (CO2_IP) in these ponds was dominated by sediments. A significantly larger mean porewater CO2 concentrations, diffusive fluxes and production rate were observed in the MRE ponds than those in the JRE ponds, which could be attributed to the lower water salinity and a larger source of carbon substrates in the former estuary. Considering a total surface area of 6.63 × 103 km2 across the mariculture ponds in subtropical estuaries, it is estimated conservatively that approximately 100 Gigagram (Gg) of dissolved organic carbon and 190 Gg of dissolved inorganic carbon were transported annually from the mariculture ponds into China's coastal areas. Because of the substantial supply of dissolved carbon, the adjacent coastal waters receiving effluent discharge from the mariculture ponds could become “hotspots” of CO2 emissions. Our results highlight the role of aquaculture pond as a major CO2 source in China's coastal areas, and effective actions are needed to alleviate the greenhouse gas (GHG) emissions from these ponds
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Production and uptake of dissolved carbon, nitrogen, and phosphorus in overlying water of aquaculture shrimp ponds in subtropical estuaries, China
Water quality deterioration can adversely affect the long-term sustainability of aquaculture industry. Understanding the processes of nutrient regeneration and uptake is important for improving water quality and the overall ecosystem health of aquaculture system. In spite of the importance of dissolved nutrients (DOC, DIC, N-NO , N-NH , and P-PO ) in governing water quality and ecosystem functioning, the spatiotemporal variations in the production and uptake of dissolved nutrients in aquaculture ponds is still poorly understood. In this study, the nutrient production and uptake rates in the overlying water were quantified among different shrimp growth stages in the aquaculture ponds in the Min River Estuary (MRE) and Jiulong River Estuary (JRE), southeast China. Significant differences in the nutrient production and uptake rates in the overlying water were observed among the three growth stages and two estuaries. The temporal variations of DOC and DIC production rates in both estuarine ponds closely followed the seasonal cycle of temperature, while the difference in DOC and DIC production rates between the two estuaries was likely caused by differences in water salinity. The changes in the production and uptake rates of dissolved inorganic nitrogen (N-NO and N-NH ) and P-PO in the water column over time were partly related to the interactions between thermal conditions and phytoplankton biomass (e.g., chlorophyll a concentrations) in the ponds. Our results demonstrate the complex dynamics and environmental risk of dissolved nutrients in subtropical shrimp ponds, and call for a more effective management of nutrient-laden wastewater in safeguarding the long-term sustainability of aquaculture production
Lattice study on and X(3872)
Properties of charmonium are investigated in quenched
lattice QCD. The mass of is determined to be 3.80(3) GeV, which is
close to the mass of -wave charmonium and in agreement with
quark model predictions. The transition width of
is also obtained with a value keV. Since the possible
assignment to X(3872) has not been ruled out by experiments, our results help
to clarify the nature of X(3872).Comment: 15 pages, 8 figures. typos, grammatical errors and some references
corrected, redundant discussions deleted, conclusion does not change.
published versio
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Large fine‐scale spatiotemporal variations of CH4 diffusive fluxes from shrimp aquaculture ponds affected by organic matter supply and aeration in Southeast China
Mariculture shrimp ponds are important CH4 sources to the atmosphere. However, the spatiotemporal variations of CH4 concentration and flux at fine spatial scales in mariculture ponds are poorly known, particularly in China, worlds largest aquaculture producer. In this study, the plot‐scale spatiotemporal variations of water CH4 concentration and flux, both within and among ponds, were researched in shrimp ponds in Shanyutan wetland, Min River Estuary, Southeast China. The average water CH4 concentration and diffusion flux across the water‐air interface in the shrimp ponds over the shrimp aquaculture period varied from 2.29 ± 0.29 to 50.48 ± 20.91 μM and from 0.09 ± 0.01 to 2.32 ± 0.95 mmol·m−2·hr−1, respectively. The CH4 emissions from the estuarine ponds varied greatly between seasons, with peaks in August and September, which was similar to the trend of water temperature and dissolved oxygen concentrations. There was no remarkable difference in CH4 concentration and flux between shrimp ponds but significantly spatiotemporal differences in CH4 concentration and flux within the ponds. Significantly higher emissions occurred in the feeding zone, accounting for approximately 60% of total CH4 emission flux, while much lower CH4 emissions appeared in aeration zone, contributing 14% to total flux. This study suggests the importance of considering spatiotemporal variation in the whole‐pond estimates of CH4 concentration and flux. In light of such high spatial variation within ponds, improving aeration and feed utilization efficiency would help to mitigate CH4 emissions from mariculture ponds
2-Chloro-1-(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl)ethanone
In the title compound, C9H10ClNOS, the dihedral angle between the planar thiophene ring and 2-chloroethane moiety (r.m.s deviations of 0.003 and 0.015 Å, respectively) is 45.79 (6)°. The tetrahydropyridine ring adopts a half-chair conformation. The crystal packing reveals intermolecular C—H⋯O interactions
Neutron star phase transition as the origin for the fast radio bursts and soft gamma-ray repeaters of SGR J1935+2154
Magnetars are believed as neutron stars (NSs) with strong magnetic fields.
X-ray flares and fast radio bursts (FRBs) have been observed from the magnetar
(soft gamma-ray repeater, SGR J1935+2154). We propose that the phase transition
of the NS can power the FRBs and SGRs.Based on the equation of state provided
by the MIT bag model and the mean field approximation, we solve the
Tolman-Oppenheimer-Volkoff equations to get the NS structure. With spin-down of
the NS, the hadronic shell gradually transfers to the quark shell.The
gravitational potential energy released by one time of the phase transition can
be achieved. The released energy, time interval between two successive phase
transitions, and glitch are all consistent with the observations of the FRBs
and the X-ray flares from SGR J1935+2154. We conclude that the phase transition
of an NS is a plausible mechanism to power the SGRs as well as the repeating
FRBs.Comment: 11 pages, 3 figure
The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study
Background
Calmodulin (CaM) plays an important role in Ca2+-dependent signal transduction. Ca2+ binding to CaM triggers a conformational change, forming a hydrophobic patch that is important for target protein recognition. CaM regulates a Ca2+-dependent inactivation process in store-operated Ca2+entry, by interacting Orai1. To understand the relationship between Ca2+-induced hydrophobicity and CaM/Orai interaction, chimera proteins constructed by exchanging EF-hands of CaM with those of Troponin C (TnC) are used as an informative probe to better understand the functionality of each EF-hand. Results
ANS was used to assess the context of the induced hydrophobic surface on CaM and chimeras upon Ca2+ binding. The exchanged EF-hands from TnC to CaM resulted in reduced hydrophobicity compared with wild-type CaM. ANS lifetime measurements indicated that there are two types of ANS molecules with rather distinct fluorescence lifetimes, each specifically corresponding to one lobe of CaM or chimeras. Thermodynamic studies indicated the interaction between CaM and a 24-residue peptide corresponding to the CaM-binding domain of Orail1 (Orai-CMBD) is a 1:2 CaM/Orai-CMBD binding, in which each peptide binding yields a similar enthalpy change (ΔH = −5.02 ± 0.13 kcal/mol) and binding affinity (Ka = 8.92 ± 1.03 × 105 M−1). With the exchanged EF1 and EF2, the resulting chimeras noted as CaM(1TnC) and CaM(2TnC), displayed a two sequential binding mode with a one-order weaker binding affinity and lower ΔH than that of CaM, while CaM(3TnC) and CaM(4TnC) had similar binding thermodynamics as CaM. The dissociation rate constant for CaM/Orai-CMBD was determined to be 1.41 ± 0.08 s−1 by rapid kinetics. Stern-Volmer plots of Orai-CMBD Trp76 indicated that the residue is located in a very hydrophobic environment but becomes more solvent accessible when EF1 and EF2 were exchanged. Conclusions
Using ANS dye to assess induced hydrophobicity showed that exchanging EFs for all Ca2+-bound chimeras impaired ANS fluorescence and/or binding affinity, consistent with general concepts about the inadequacy of hydrophobic exposure for chimeras. However, such ANS responses exhibited no correlation with the ability to interact with Orai-CMBD. Here, the model of 1:2 binding stoichiometry of CaM/Orai-CMBD established in solution supports the already published crystal structure
Identification and analysis of phosphorylation status of proteins in dormant terminal buds of poplar
<p>Abstract</p> <p>Background</p> <p>Although there has been considerable progress made towards understanding the molecular mechanisms of bud dormancy, the roles of protein phosphorylation in the process of dormancy regulation in woody plants remain unclear.</p> <p>Results</p> <p>We used mass spectrometry combined with TiO<sub>2 </sub>phosphopeptide-enrichment strategies to investigate the phosphoproteome of dormant terminal buds (DTBs) in poplar (<it>Populus simonii × P. nigra</it>). There were 161 unique phosphorylated sites in 161 phosphopeptides from 151 proteins; 141 proteins have orthologs in <it>Arabidopsis</it>, and 10 proteins are unique to poplar. Only 34 sites in proteins in poplar did not match well with the equivalent phosphorylation sites of their orthologs in <it>Arabidopsis</it>, indicating that regulatory mechanisms are well conserved between poplar and <it>Arabidopsis</it>. Further functional classifications showed that most of these phosphoproteins were involved in binding and catalytic activity. Extraction of the phosphorylation motif using Motif-X indicated that proline-directed kinases are a major kinase group involved in protein phosphorylation in dormant poplar tissues.</p> <p>Conclusions</p> <p>This study provides evidence about the significance of protein phosphorylation during dormancy, and will be useful for similar studies on other woody plants.</p
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