Planning and control of a hybrid vacuum-forming system based on screw-pin tooling

This paper introduced planning and control of a hybrid vacuum-forming machine system (HAVES). The HAVES was developed to integrate CAD/CAM, screw-pin tooling, CNC, vacuum forming and optical measurement device together to produce vacuum forming components. The screw-pin tooling was employed to replace traditional dedicated solid tooling and its reconfigurability provided the HAVES with advantages in rapid producing small batch and mass customization products. The whole control system of the HAVES is divided into two parts: one for screw-pin tooling adjustment and machining by using CNC control, and the other for vacuum forming by using PLC control. The detail design of the control system was discussed

miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit +

The low survival rate of cardiac stem cells (CSCs) in the infarcted myocardium hampers cell therapy for ischemic cardiomyopathy. MicroRNA-21 (miR-21) and one of its target proteins, PTEN, contribute to the survival and proliferation of many cell types, but their prosurvival effects in c-kit+ CSC remain unclear. Thus, we hypothesized that miR-21 reduces hydrogen peroxide- (H2O2-) induced apoptosis in c-kit+ CSC and estimated the contribution of PTEN/PI3K/Akt signaling to this oxidative circumstance. miR-21 mimics efficiently reduced H2O2-induced apoptosis in c-kit+ CSC, as evidenced by the downregulation of the proapoptosis proteins caspase-3 and Bax and upregulation of the antiapoptotic Bcl-2. In addition, the gain of function of miR-21 in c-kit+ CSC downregulated the protein level of PTEN although its mRNA level changed slightly; in the meantime, miR-21 overexpression also increased phospho-Akt (p-Akt). The antiapoptotic effects of miR-21 were comparable with Phen (bpV), the selective inhibitor of PTEN, while miR-21 inhibitor or PI3K’s inhibitor LY294002 efficiently attenuated the antiapoptotic effect of miR-21. Taken together, these results indicate that the anti-H2O2-induced apoptosis effect of miR-21 in c-kit+ CSC is contributed by PTEN/PI3K/Akt signaling. miR-21 could be a potential molecule to facilitate the c-kit+ CSC therapy in ischemic myocardium

Overexpression of the FBA and TPI genes promotes high production of HDMF in Zygosaccharomyces rouxii

4-Hydroxy-2,5-dimethyl-3 (2H)-furanone (HDMF) is widely used in the food industry as a spice and flavoring agent with high market demand. In this study, fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) were overexpressed in Zygosaccharomyces rouxii in the form of single and double genes, respectively, via electroporation. High-yield HDMF-engineered yeast strains were constructed by combining the analysis of gene expression levels obtained by real-time fluorescence quantitative PCR technology and HDMF production measured by HPLC. The results showed that there was a significant positive correlation between the production of HDMF and the expression levels of the FBA and TPI genes in yeast; the expression levels of the FBA and TPI genes were also positively correlated (p < 0.05). Compared with the wild type (WT), the engineered strains F10-D, T17-D, and TF15-A showed marked increases in HDMF production and FBA and TPI gene expression (p < 0.05) and exhibited great genetic stability with no obvious differences in biomass or colony morphology. In addition, the exogenous addition of d-fructose promoted the growth of Z. rouxii. Among the engineered strains, when fermented in YPD media supplemented with d-fructose for 5 days, TF15-A (overexpressing the FBA and TPI genes) generated the highest HDMF production of 13.39 mg/L, which is 1.91 times greater than that of the wild-type strain. The results above indicated that FBA and TPI, which are key enzymes involved in the process of HDMF biosynthesis by Z. rouxii, positively regulate the synthesis of HDMF at the transcriptional level. d-fructose can be used as a precursor for the biosynthesis of HDMF by engineered yeast in industrial production

A New Perspective on the Evaluation of Urbanization Sustainability: Urban Health Examination

In recent decades, urbanization around the world has become an unavoidable trend. However, rapid urbanization has brought about a number of “urban diseases” which have considerable influence on sustainable urbanization. In order to diagnose urban problems, this study introduces a new perspective for the evaluation of urbanization sustainability named “urban health examination (UHE)” based on the urban lifeform theory which treats a city as a human body system. Then, an evaluation index system of UHE incorporating eight dimensions is constructed by referring to the existing authoritative indicator systems globally. Furthermore, a deviation maximization method and obstacle analysis method are integrated to evaluate urban development level and diagnose the urban diseases. Finally, in order to verify the feasibility of the UHE methodological framework, an empirical study was conducted in Wuhan, Central China. The results show that (1) the main urban diseases suffered by Wuhan City in 2010–2019 include traffic congestion, waterlogging, unsafe production, insufficient technological power, and excessive urban development; (2) the evolution mechanism of urban diseases in Wuhan has been explored. This study proposes a methodological framework of UHE which can successfully diagnose urban diseases, so that local urban managers adopt tailored strategies to prevent urban diseases and further achieve sustainable urban development goals