Analysis of leukocyte rolling and migration--using inhibitors in the undisturbed microcirculation of the rat mesentery--on inflammatory stimulation.

AIM: Our aim was to develop a method of migration analysis using the undisturbed microcirculation of rat mesentery, and using the new method, analyze leukocyte migration in casein-induced inflammation. METHOD: Sprague Dawley (SD) rats were injected with tumor necrosis factor (TNF) alpha, interleukin (IL)-1alpha, or casein intraperitoneally. Following this, the rats were sacrificed and the mesentery tissue removed was fixed and stained with Giemsa. The leukocytes were counted as a rolling index in the venules and as a migration index in the perivascular area. RESULTS: There was no relation between the diameter of venules and leukocyte migration. The time change curves of leukocyte activity in casein inflammation show about a 1 h difference between rolling and migration. From inhibitor experiments of casein-induced migration at 2 h, it has been suggested that selectin-related rolling is necessary. Platelet-activating factor (PAF) also appears partially involved. CONCLUSION: The improved undisturbed microcirculation method is helpful not only for rolling analysis but also in analysis of leukocyte migration. Casein inflammation analyzed using this method revealed that rolling is necessary and also suggested that partial involvement of PAF is necessary for pathogenesis of leukocyte extravasations

Desempeños del alumnado de Educación Secundaria en la evaluación de una investigación científica en el contexto de la industria láctea

En este estudio se analizan los desempeños de estudiantes de 2.º, 3.º y 4.º de ESO (14, 15 y 16 años) que cursan la asignatura de Física y química, cuando evalúan el diseño de una investigación sobre un problema de precipitación identificado en la industria láctea. Los desempeños se examinan por separado para cada dimensión del diseño: identificación de la cuestión objeto de investigación, formulación de hipótesis, planificación de la investigación y selección del criterio de finalización. Para el análisis de resultados, se recogen sus propuestas, en las que han de estar desarrolladas las siguientes tareas: seleccionar la mejor opción, justificar su elección y modificar la opción alternativa para convertirla en correcta. Los resultados menos adecuados corresponden a las dos últimas dimensiones citadas.In this paper we analyse the performance of 8th, 9th and 10th grade students (14, 15 and 16 years old respectively), attending Physics and Chemistry lessons, when assessing the design of scientific research on a precipitation problem identified in the dairy industry. Students' performances are examined separately for each dimension involved in the design: identifying the issue to be studied, posing the hypotheses, planning the research and selecting the ending criterion. For the analysis, we collect participants' individual proposals, which have to include the following tasks: selecting the best option, justifying their choice and modifying the alternative option so that it will be correct. The less adequate results correspond to the last two dimensions