A PMMA valveless micropump using electromagnetic actuation

We have fabricated and characterized a polymethylmethacrylate (PMMA) valveless micropump. The pump consists of two diffuser elements and a polydimethylsiloxane (PDMS) membrane with an integrated composite magnet made of NdFeB magnetic powder. A large-stroke membrane deflection (~200μm) is obtained using external actuation by an electromagnet. We present a detailed analysis of the magnetic actuation force and the flow rate of the micropump. Water is pumped at flow rates of up to 400µl/min and backpressures of up to 12mbar. We study the frequency-dependent flow rate and determine a resonance frequency of 12 and 200Hz for pumping of water and air, respectively. Our experiments show that the models for valveless micropumps of A. Olsson et al. (J Micromech Microeng 9:34, 1999) and L.S. Pan et al. (J Micromech Microeng 13:390, 2003) correctly predict the resonance frequency, although additional modeling of losses is necessar

Plastic micropumps using ferrofluid and magnetic membrane actuation

This paper presents a simple and low-cost prototyping technology for the realization of integrated micropumps in polymethylmethacrylate (PMMA). The three-dimensional (3D) micropumps consist of stacks of structured PMMA layers, which are either realised with precision milling tools for the more complex parts, or fabricated using the powder blasting technique for channel-type structures. We integrate silicone membranes into the chip to realize check-valves or use dynamic diffuser valves. We use two different types of magnetic actuation external to the micropump: (i) an external magnet displaces a ferrofluid liquid plug that plays the role of a piston in a channel; (ii) an external coil actuates an integrated magnetic membrane, consisting of NdFeB magnetic powder in a polydimethylsiloxane (PDMS) matrix

A ferrofluid micropump for lab-on-a-chip applications

A disposable micropump is presented that uses the piston actuation principle and relies on the magnetic properties of a ferrofluid, a colloidal suspension of nanosize ferromagnetic particles. The cost effective micropump consists of 7 bonded layers of polymethylmetacrylate (PMMA) that are either micromachined or structured by powder blasting. Two silicone check-valves are also integrated in the microchip. External dimensions of our prototype are 36 mm x 22 mm x 5 mm. The magnetic liquid plug is externally actuated by a motorized permanent magnet. Water has been successfully pumped at a flow rate of 30 µL/min without backpressure; pumping is demonstrated up to a backpressure of 25 mbar

Electromagnetically Actuated Ball Valve Micropumps

We present two types of oscillating diaphragm micropumps configured with passive ball valves and using electromagnetic actuation. One type is made out of poly(methyl methacrylate) (PMMA), while the other one is made out of borosilicate glass. Both were produced using the powder blasting microfabrication method. The pumping resonant frequency was measured to be within the range of 20 – 30 Hz for both prototypes. At the resonance, a maximum back-pressure of 280 mbar and a maximum water flow rate of about 5 mL/min were obtained. The experimental results can be well described by a simple hydrodynamic model of the system

Pumping of mammalian cells with a nozzle-diffuser micropump

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications