Do unfair experiences contribute to anger and hostility in young children? : young children's perception of unfairness and its associated feelings : a thesis presented in partial fulfilment of the requirements for the degree of Master of Arts in Psychology

Being able to cope in emotionally demanding situations is a necessary skill for both children and adults. Parents use many disciplinary techniques to help children manage their misbehaviour and overtly expressed negative emotions. Although numerous parenting strategies have been described by researchers and professionals, there are limited numbers of studies looking at aspects of disciplinary practices from young children's perspectives. By understanding children's affective responses to discipline practices, it is possible that parents can provide more effective help for their children, and reduce negative moods such as hostility. Based on Evans' model of the development of hostility, the present study investigated how young children's experiences of unfair treatment might influence their predominant affective expression or attitudes toward a parent who is being unfair. Thirty-two children, aged between five and nine years, were recruited from local primary schools and a summer holiday programme. Using a story about unfairness, the children were able to make judgements about the story and report feelings associated with such experiences. Three original tasks were introduced as an indirect measure of level of hostility in response to the experiences of unfair treatment. Although statistical analysis supported the hypotheses that hostility in children increases as a result of unfair experiences, judging from their verbal responses, there is a complex interactive effect of the mother-child relationship on children's feeling

Spin effects in single-electron transport through carbon nanotube quantum dots

We investigate the total spin in an individual single-wall carbon nanotube quantum dot with various numbers of electrons in a shell by using the ratio of the saturation currents of the first steps of Coulomb staircases for positive and negative biases. The current ratio reflects the total-spin transition that is increased or decreased when the dot is connected to strongly asymmetric tunnel barriers. Our results indicate that total spin states with and without magnetic fields can be traced by this method.Comment: 5pages, 5figures, accepted for publication in Phys. Rev.

Growth Inhibition of Cultured Human Liver Carcinoma Cells by Ki-energy (Life-energy): Scientific Evidence for Ki-effects on Cancer Cells

‘Ki-energy’ (life-energy) is believed to increase the immune activity of its practitioners. It has also been shown to cause neuropsychological effects. We undertook this study to obtain objective and scientific evidence as to whether or not a ‘Ki-effect’ could inhibit the growth of cultured cancer cells. Cultured human liver carcinoma cells, HepG2, were used. A Japanese Ki-expert held his fingers toward the cells in culture dishes for 5 or 10 min. After culturing for 24 h, we measured cell numbers, protein concentration per cell, certain mRNA expressions and the synthesis of regucalcin. The results were compared with those for control cells (non-treated cells). We found that the number of cells in the Ki-exposed groups were less than those in the controls by 30.3 and 40.6% with 5 and 10 min Ki-exposure, respectively. The protein content per cell in the Ki-exposed groups (5 and 10 min) was higher than that in the control groups by 38.8 and 62.9%, respectively. These results were statistically significant. Using RT–PCR, we found that the mRNA expression for c-myc, a tumor stimulator gene, was decreased, while that for regucalcin, which suppresses DNA synthesis, was increased. Our molecular biological studies and mathematical model analysis demonstrated that Ki-energy inhibited cancer cell division. The data also indicate that the Ki-effects involve some form of infrared radiation from the human body. This study suggests the possibility that Ki-energy may be beneficial for cancer patients because it suppresses cancer cell growth, and at the same time, it stimulates immune functions of the patients

Ki-energy (Life-energy) Stimulates Osteoblastic Cells and Inhibits the Formation of Osteoclast-like Cells in Bone Cell Culture Models

Some practitioners of the Nishino Breathing Method (NBM) were found to have a higher bone density than the average values of age- and gender-matched non-practitioners. Using bone cell culture models, we investigated a possible mechanism behind this observation. For the study of bone mineralization, we performed the following two experiments using cultured osteoblastic MC3T3-E1 cells: (i) Kozo Nishino, a Japanese Ki expert, sent Ki-energy to the cells once for 5 or 10 min after they were seeded in culture dishes in the presence of 10% fetal bovine serum (FBS). They were incubated for 72 h and the cells were counted. The number in the dish with 10-min Ki-exposure was significantly greater than that in the control (P < 0.01 with n = 8). We performed a reverse transcription-polymerase chain reaction (RT–PCR) study using these cells, but the mRNA expressions did not change significantly. (ii) After cells were incubated for 72 h without Ki-exposure (in the presence of FBS), they were further cultured for 48 h (in the absence of FBS) to promote differentiation. At the beginning of the second culture stage, Ki was applied once for 10 min. After 48 h, RT–PCR was performed. The mRNA expressions which are related to bone mineralization, such as Runx2, α1(I) collagen, alkaline phosphatase and osteocalcin, increased significantly (P < 0.05 and n = 4 for all). For the bone resorption study, we used mouse marrow cultures, which can form osteoclast-like cells in the presence of (1–34) parathyroid hormone (PTH), and stimulate resorption. We exposed these cells to Ki-energy twice for the duration of 5 or 10 min on day 0 and day 4. On day 7, the cells were counted. The number of osteoclast-like cells in dishes with Ki exposure was significantly smaller than those in control dishes (P < 0.05 with n = 5). The difference between 5-min exposure and 10-min exposure was not statistically significant. All of our data suggest that the Ki-effect on osteoporosis should be further explored