Diabetes mellitus itself increases cardio- cerebrovascular risk and renal complications in primary aldosteronism

This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Clinical Endocrinology & Metabolism following peer review. The version of record Aya Saiki, Michio Otsuki, Daisuke Tamada, Tetsuhiro Kitamura, Iichiro Shimomura, Isao Kurihara, Takamasa Ichijo, Yoshiyu Takeda, Takuyuki Katabami, Mika Tsuiki, Norio Wada, Toshihiko Yanase, Yoshihiro Ogawa, Junji Kawashima, Masakatsu Sone, Nobuya Inagaki, Takanobu Yoshimoto, Ryuji Okamoto, Katsutoshi Takahashi, Hiroki Kobayashi, Kouichi Tamura, Kohei Kamemura, Koichi Yamamoto, Shoichiro Izawa, Miki Kakutani, Masanobu Yamada, Akiyo Tanabe, Mitsuhide Naruse, Diabetes Mellitus Itself Increases Cardio-Cerebrovascular Risk and Renal Complications in Primary Aldosteronism, The Journal of Clinical Endocrinology & Metabolism, Volume 105, Issue 7, July 2020, Pages e2531–e2537 is available online at: https://doi.org/10.1210/clinem/dgaa177

Development and evaluation of novel salt-tolerant Eucalyptus trees by molecular breeding using an RNA-binding-protein gene derived from common ice plant (Mesembryanthemum crystallinum L.)

The breeding of plantation forestry trees for the possible afforestation of marginal land would be one approach to addressing global warming issues. Here, we developed novel transgenic Eucalyptus trees (Eucalyptus camaldulensis Dehnh.) harbouring an RNA‐Binding‐Protein (McRBP) gene derived from a halophyte plant, common ice plant (Mesembryanthemum crystallinum L.). We conducted screened‐house trials of the transgenic Eucalyptus using two different stringency salinity stress conditions to evaluate the plants’ acute and chronic salt stress tolerances. Treatment with 400 mM NaCl, as the high‐stringency salinity stress, resulted in soil electrical conductivity (EC) levels >20 mS/cm within 4 weeks. With the 400 mM NaCl treatment, >70% of the transgenic plants were intact, whereas >40% of the non‐transgenic plants were withered. Treatment with 70 mM NaCl, as the moderate‐stringency salinity stress, resulted in soil EC levels of approx. 9 mS/cm after 2 months, and these salinity levels were maintained for the next 4 months. All plants regardless of transgenic or non‐transgenic status survived the 70 mM NaCl treatment, but after 6‐month treatment the transgenic plants showed significantly higher growth and quantum yield of photosynthesis levels compared to the non‐transgenic plants. In addition, the salt accumulation in the leaves of the transgenic plants was 30% lower than that of non‐transgenic plants after 15‐week moderate salt stress treatment. There results suggest that McRBP expression in the transgenic Eucalyptus enhances their salt tolerance both acutely and chronically

Heterogeneous circulating miRNA profiles of PBMAH

ObjectivePrimary bilateral macronodular adrenal hyperplasia (PBMAH), a rare cause of Cushing syndrome, is often diagnosed as a bilateral adrenal incidentaloma with subclinical cortisol production. Circulating microRNAs (miRNAs) are a characteristic of adrenocortical adenomas, but miRNA expression in PBMAH has not been investigated. We aimed to evaluate the circulating miRNA expression in patients with PBMAH and compare them with those in patients with non-functioning adrenocortical adenoma (NFA) and cortisol-producing adrenocortical adenoma (CPA).MethodsmiRNA profiling of plasma samples from four, five, and five patients with NFA, CPA, and PBMAH, respectively, was performed. Selected miRNA expressions were validated using quantitative RT-PCR.ResultsPBMAH samples showed distinct miRNA expression signatures on hierarchical clustering while NFA and CPA samples were separately clustered. PBMAH was distinguished from the adenoma group of NFA and CPA by 135 differentially expressed miRNAs. Hsa-miR-1180-3p, hsa-miR-4732-5p, and hsa-let-7b-5p were differentially expressed between PBMAH and adenoma (P = 0.019, 0.006, and 0.003, respectively). Furthermore, PBMAH could be classified into two subtypes based on miRNA profiling: subtype 1 with a similar profile to those of adenoma and subtype 2 with a distinct profile. Hsa-miR-631, hsa-miR-513b-5p, hsa-miR-6805-5p, and hsa-miR-548av-5p/548k were differentially expressed between PBMAH subtype 2 and adenoma (P = 0.027, 0.027, 0.027, and 1.53E-04, respectively), but not between PBMAH, as a whole, and adenoma.ConclusionCirculating miRNA signature was identified specific for PBMAH. The existence of subtype-based miRNA profiles may be associated with the pathophysiological heterogeneity of PBMAH

ルーブリック オ シヨウシタ カンリ エイヨウシ ヨウセイ カテイ ニオケル リンチ ジッシュウ ノ ヒョウカ

【目的】管理栄養士養成課程において、学生の臨地実習の事前事後の学修成果について到達度評価に適しているとされるルーブリックを用いて評価し、総合演習を含む事前事後指導の在り方を振り返る。【方法】本校において設定された管理栄養士養成課程におけるルーブリックによる自己評価を臨地実習の事前と事後に実施し学修成果を分析した。【結果及び考察】臨地実習前の評価では、３年生女性では、主体性及びコミュニケーション力を除いた４項目（課題設定・解決力、情報活用力、知識と情報の統合力、職業観）で評価規準３または４と評価した学生が少なく、知識と情報の統合力の評価規準を１と評価した学生が２割いた。３年生男性は、コミュニケーション力の評価は高いが、その他の５項目（主体性、課題設定・解決力、情報活用力、知識と情報の統合力、職業観）の評価が低かった。またコミュニケーション力以外の項目で３年生の女性と同様に評価規準４と評価した割合が低かった。４年生の女性は、知識と情報の統合力の項目を除いた５項目（主体性、コミュニケーション力、課題設定・解決力、情報活用力、職業観）について半数以上の学生が評価規準３または４と評価し３年生女性より評価規準が高かった。男性については、該当人数が４名と少ないが、すべての項目で評価規準２または３と評価し評価規準４と評価した学生はいなかった。臨地実習後の評価については、３年生女性は課題設定・解決力が若干低かったが、それ以外の５項目について評価規準３または４と評価した学生が多くなり、３年生の男性は、主体性及び情報活用力以外の４項目について評価規準３または４と評価した学生が多かった。４年生女性の事後評価では、評価基準の各項目において評価規準３と評価した割合が高くなり評価規準４と評価した割合が減少した。４年生の男性は主体性及び情報活用力を除いて全員が評価規準３または４と評価した。どの項目も実習後の評価は評価規準３または４に評価した学生が増加し、その割合は６割強以上となり、管理栄養士として備えたい必要な力についておおむね獲得できたこと、本大学の学生は社会における管理栄養士としての資質（知識、技術、態度）についての理解が臨地実習後におおむね獲得できたという評価となり、今回用いた「管理栄養士課程における臨地実習ルーブリック」により臨地実習の教育効果を可視化できたととらえることができた

サークライン型蛍光灯の高周波点灯

Measurements are carried out on a comparison between high frequency lighting and normal lighting of a doughnut-type fluorescent lamp. A toroidal coil is installed around the lighting tube. High frequency power (13.65MHz, 30W) is supplied to the toroidal coil through an automatic matching circuit. Compared with normal lighting, almost same luminous intensity is obtained in the high frequency lighting, when the coil input impedance matches with the oscillator output impedance through the matching circuit

Poster Session

International Symposium on Tumor Biology in Kanazawa & Symposium on Drug Discoverry in Academics 2014 [DATE]: January 23(Thu)-24(Fri),2014, [Place]:Kanazawa Excel Hotel Tpkyu, Kanazawa, Japan, [Organizers]:Kanazawa Association of Tumor Biologists / Cancer Research Institute, Kanazawa Universit

Clinical characteristics of Japanese patients with axial spondyloarthritis, and short-term efficacy of adalimumab

AbstractBackgroundAnkylosing spondylitis (AS) is rarer in Japan than in Europe, probably because the European criteria, not well known by Japanese general physicians, regard AS as a progressive stage of axial spondyloarthritis (SpA). HLA-B27 is an important diagnostic marker of SpA; however, the incidence of the HLA-B27 allele is as low as 0.4% in Japan. For Japanese SpA patients, other HLA alleles and clinical findings are required for earlier definitive diagnosis, for determining appropriate treatment timing, and for disease monitoring.MethodsWe investigated the HLA-B alleles of 36 patients clinically diagnosed with SpA. For 8 axial SpA patients we evaluated the short-term efficacy of subcutaneous adalimumab injections (40mg every other week for ≥11months). Treatment efficacy was evaluated by use of the Bath Ankylosing Spondylitis Activity Index (BASDAI) score, and serum TNF-α and IL-6 levels were measured pre and post-treatment.ResultsAmong the 36 Japanese SpA patients, the HLA-B27 allele occurred infrequently (5.6%) whereas the HLA-B44 and 61 alleles were the most frequently detected (25.0%). We also detected severe bamboo spine on radiography in the absence of the HLA-B27 allele. All 8 patients with axial SpA experienced significant symptom improvement after adalimumab treatment; the HLA-B27 allele was absent from these patients. Serum TNF-α and IL-6 levels were elevated in cases with remarkable inflammatory pain and high disease activity. These cytokines decreased after therapy, however. Most patients with normal cytokine levels at baseline retained these low levels.ConclusionsThe findings reveal the short-term efficacy of adalimumab. The remarkably low incidence of HLA-B27 among our patients indicates that HLA-B distribution is different from that in other countries. Serum TNF-α and IL-6 levels were not effective as biomarkers for cases without high disease activity, and further research with larger samples is needed. The efficacy of TNF blockers, however, suggested a potential localized TNF effect was present among SpA patients