Cascade Molecule–Particle–Molecule Self-Assemblies for Fabricating Narrowly Size-Distributed Polymeric Superparticles with a Bicontinuous Nanostructure

Broader developments of nanoscience and nanotechnology require complexly but regularly structured nanoparticles whose fabrications in turn pose a great challenge to nanoscience and nanotechnology. In this communication, we report a new and robust method with a clear mechanism for fabricating narrowly size-distributed superparticles with a bicontinuous inner structure. The processes for the fabrication include: molecular self-assembly of a triblock copolymer in its selective solvent into the core–shell–corona micelles, the self-limited aggregation of the micelles (particles) into narrowly size-distributed superparticles constructed by the integrated micelles, and the final molecular self-assembly confined within the superparticles into cylinders that are crowded and interconnected to form the bicontinuous nanostructure; the molecular self-assembly into the micelles, the self-limited aggregation of the particles (i.e., the micelles), and the further molecular self-assembly within the superparticles occurred in a cascade manner

Additional file 2 of Circular RNA hsa_circ_0067842 facilitates tumor metastasis and immune escape in breast cancer through HuR/CMTM6/PD-L1 axis

Additional file 2: Table S1. Correlation between hsa_circ_0067842 expression and clinicopathological characteristics; Table S2. Sequences of primers used for qRT-PCR in this study; Table S3. Sequences of probes used in this study; Table S4. Antibodies for Immunoblotting in this study; Table S5. Oligonucleotides used in the stud

Additional file 3 of Circular RNA hsa_circ_0067842 facilitates tumor metastasis and immune escape in breast cancer through HuR/CMTM6/PD-L1 axis

Additional file

Additional file 1 of Circular RNA hsa_circ_0067842 facilitates tumor metastasis and immune escape in breast cancer through HuR/CMTM6/PD-L1 axis

Additional file 1: Figure S1. The genomic locus and characteristics of hsa_circ_0067842. A Schematic diagram showing the conservation of hsa_circ_0067842. B Schematic diagram showing the genomic locus and the flanking introns with ALU sequence of hsa_circ_0067842 (upper). Complementary ALU sequence (FLAM_A-AluJb and FLAM_A-AluSg) in the flanking introns of SMC4 exon 12 to exon 17 (lower). C Expression of SMC4 across diverse normal human tissues from GTEx ( https://www.gtexportal.org/home/index.html ); Figure S2. The effects of hsa_circ_0067842 on proliferation of BC cells in vitro. A qRT-PCR detecting the expression of SMC4 in BC cells after transfection of the NC or hsa_circ_0067842 plasmid and siRNAs si-NC, si-hsa_circ_0067842-1, si-hsa_circ_0067842-2. B CCK-8 assays in hsa_circ_0067842-overexpressing MCF-7 or hsa_circ_0067842-depleted MDA-MB-231 cells. C Colony formation assays in hsa_circ_0067842-overexpressing MCF-7 or hsa_circ_0067842-depleted MDA-MB-231 cells; Figure S3. The relationship among hsa_circ_0067842, HuR, and CMTM6. A The binding sites of hsa_circ_0067842 and HuR predicted by RBP suite. B Secondary structure of hsa_circ_0067842 and high probable binding regions. C The transfection efficiency of si-CMTM6s in BT-549 and MCF-7 cells was verified by qRT-PCR and western blot. D The expression of CMTM6 in rescue experiments was assessed by qRT-PCR and western blo

Highly Selective Detection of 5‑Methylcytosine in Genomic DNA Based on Asymmetric PCR and Specific DNA Damaging Reagents

DNA methylation is a significant epigenetic modification of the genome that is involved in regulating many cellular processes. An increasing number of human diseases have been discovered to be associated with aberrant DNA methylation, and aberrant DNA methylation has been deemed to be a potential biomarker for diseases such as cancers. A safe, nontoxic, and sensitive method for accurate detection of 5-methylcytosine in genomic DNA is extremely useful for early diagnosis and therapy of cancers. In this paper, we established a novel system to detect 5-methylcytosine, which is based on bisulfite treatment, asymmetric PCR, and specific DNA damaging reagents. Our method could be used for identifying the loci of 5mC in genomic DNA and detecting the DNA methylation levels in tissues as well