Bazı Kolza Hatlarının Diyarbakır Şartlarındaki Verim ve Verim Unsurlarının Saptanması

Bu araştırma Diyarbakır koşullarında kolza tarımına uygun yüksek performanslı bazı yeni hatların belirlenmesi amacıyla yürütülmüştür. Araştırmada materyal olarak; Turan, Triston, H 607245, Es Hydromel, Elvis, Lıcord, Calıfornium, Excoluber, TK-ED 3-3, TK-ED 3-14, TK-ED 3-9, TK-ED 3-4, TK-ED 3-7, TK-ED 3-15, TK-ED 3-5, TK-ED 3-13, TK-ED 3-17 ve TK-ED 3-6 hat ve çeşitleri kullanılmıştır. Araştırma 2010-2011 ve 2011-2012 yetiştirme sezonlarında GAP Uluslararası Tarımsal Araştırma ve Eğitim Merkezi deneme tarlasında Tesadüf Blokları Deneme Desenine göre 3 tekerrürlü olarak yürütülmüştür. Elde edilen iki yılın ortalama verilerine göre sırasıyla; bitki boyu (145,9-174,7 cm), 1000 tane ağırlığı (3,48-4,53 g), tohum verimi (174,34-351,25 kg/da) ve yağ oranı (% 44,27-47,19) arasında değişmiştir. Sonuç olarak; incelenen özellikler göz önüne alındığında TK-ED 3-5, TK-ED 3-9 ve TK-ED 3-15 hatlarının Diyarbakır koşullarında daha iyi performans gösterdikleri görülmektedir

Delivering growth factors through a polymeric scaffold to cell cultures containing both nucleus pulposus and annulus fibrosus

WOS: 000460303600005PubMed ID: 29694659AIM: To design a novel, polyvinyl alcohol (PVA)-based polymeric scaffold that permits the controlled release of insulin-like growth factor 1 (IGF-1) /bone morphogenetic protein (BMP)-2 following intervertebral disc administration. MATERIAL and METHODS: The drug delivery system was composed of two different solutions that formed a scaffold within seconds of coming into contact with each other. Swelling, pH, and temperature tests and analysis of the controlled release of growth factors (GFs) from this system were performed. The release kinetics of the GFs were determined through enzyme-linked immunosorbent assay (ELISA). Cell proliferation and viability were monitored with microscopy and analyzed using an MTT assay and acridine orange/propidium iodide (AO/PI) staining. Chondroadherin (CHAD), hypoxia inducible factor-1 alpha (HIF-1 alpha), and collagen type II (COL2A1) gene expressions were determined with quantitative real-time polymerase chain reaction (qRT-PCR) analysis to show the effects of IGF-1/BMP-2 administration on annulus fibrosus cell (AFC)/nucleus pulposus cell (NPC) cultures. For the statistical evaluation of the obtained data, experimental groups were compared with a post hoc Tukey's test following an analysis of variance. RESULTS: The scaffold allowed for the controlled release of IGF-1 and BMP-2 in different time intervals. It was observed that as the application time increased, the number of cells and the degree of extracellular matrix development increased in AFC/NPC cultures. AO/PI staining and an MTT analysis showed that cells retained their specific morphology and continued to proliferate. It was observed that HIF-1 alpha and CHAD expression increased in a time-dependent manner, and no COL2A1 expression in the AFC/NPC cultures was observed. CONCLUSION: The designed scaffold may be used as an alternative method for intervertebral disc administration of GFs after further in vivo studies. Such prototype scaffolds may be an innovative technology in targeted drug therapies after reconstructive neurosurgical interventions

Evaluation of the effect of daptomycin, a glycopeptide agent, on intact intervertebral disc tissue

AIM: To evaluate the effects of pre- and intra-operatively administered daptomycin (DAP) on the intact human primary intervertebral disc tissue cells. MATERIAL and METHODS: Primary cell cultures were established using tissues obtained through decompressive laminectomy, traumatic intervertebral disc herniation excision, and posterior transpedicular stabilization. Non-drug-administered samples were used as a control group. The samples treated with DAP formed the study group. Molecular assays for proliferation and gene expression were performed. The obtained data were evaluated statistically, and results with a value of p<0.05 were accepted as significant. RESULTS: While no reduction was observed in the proliferation, the gene expression of intact intervertebral disc tissue cells was time-dependently decreased compared to the control group, and these results were reported to be statistically significant. CONCLUSION: This study observed the effect that a pharmaceutical preparation, which was used on intervertebral disc tissue before and after the operation, had on normal, healthy, and intact tissue. It concludes that alterations in the expression of genes involved in the anabolic and/or catabolic process, even in adjacent healthy tissue, may slow down the healing process of the damaged tissue or cause undesired cell differentiation

Are intervertebral disc tissue cells damaged when attempting to prevent thrombus formation using dabigatran, a new oral anticoagulant?

AIM: To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intact intervertebral disc tissue. MATERIAL and METHODS: Cell cultures were prepared from tissues obtained from six cases who had undergone surgery due to spinal trauma. Dabigatran, an active pharmacological agent, was applied to intact annulus fibrosus (AF)/nucleus pulposus (NP) primary cell cultures from the study group. After performing cell viability, toxicity, and proliferation tests on all cultures in the control and study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), and matrix metalloproteinase (MMP)-13 and -19 expressions were measured via a real-time polymerase chain reaction (RT-PCR). Data were analyzed statistically. RESULTS: In the proliferation assays performed on the 20th day of the study, cells in the dabigatran-supplemented group were reported to have lost 46.37% more viability than those in the control group. Expressions of all genes examined except MMP-13 were evaluated in the control group by time, but in contrast to the control group results, COMP and MMP-19 gene expressions decreased in the dabigatran-treated group. No CHAD or MMP-13 expression was noted in these cultures. CONCLUSION: The potential for a systemically applied drug to accumulate in tissue and negatively affect surrounding tissues and microstructures must be emphasized

Cognitive Impairment not only in NeuroBehcet but also for all Behcet Disease Phenotypes

Kurt, Pınar (Arel Author)Background: Cognitive impairment has been reported for Behcet's Disease (BD) patients, but studies are limited. This study is aimed to investigate neurocognitive profile of Neurobehcet Disease (NBD) patients and BD patients without neurologic involvement (BD-only) in comparison to healthy controls (HC). Patients and method: Totally 49 BD patients (11 NBD, 38 BD-only) and 32 HC were included into the study. After neurological evaluation, all subjects were given a comprehensive battery of neuropsychological tests: Self report of neuropsychological condition (by visual analogue scale-VAS, 0-100), RAVLT, Stroop, PASAT, TMT, SDMT, JLOT, DST, COWAT, ACTT, FST. Results: In the comparison of HC to NBD and BD-only subjects, a poorer performance was observed in both group of patients in almost all the neurocognitive tests, except for Stroop interference score. Both group of patients had lower scores on learning, immediate memory, working memory (ACTT), lexical fluency (COWAT) and attention (DST) comparing to healthy subjects (p <= 0.003). The differences between NBD and BD-only patients were observed on response inhibition ability (STROOP-spontaneous corrections; p=0.000, and errors; p=0.002) and learning (RAVLT-sum of five trials; p=0.002). NBD patients had further decrease on free recall (RAVLT), complex visual processing (JLOT), information processing speed (PASAT), attention (TMT-B) and psychomotor speed (TMT-A) (p <= 0.003). Conclusion: Cognitive functions of not only NBD patients but also BD-only patients were worse than control group. Compared to BD-only patients, NBD patients reveal further impairment on memory and executive functions rather than complex visual perception and verbal attention

MS *81 WM-1, Book of hours, use of Nantes. Image 313

https://repository.wellesley.edu/ms81wm1/1312/thumbnail.jp

Vitamin D status, serum lipid concentrations, and vitamin D receptor (VDR) gene polymorphisms in Familial Mediterranean fever

Vitamin D (VitD) is critical for the regulation of inflammatory processes, and VitD deficiency has been linked to several chronic inflammatory disorders. We aimed to investigate the concentrations of serum 25(OH)D3, lipid parameters, and three known VDR polymorphisms (BsmI, FokI, and TaqI) in patients with Familial Mediterranean fever (FMF), an autosomal recessive autoinflammatory disease. The study included 123 FMF patients and 105 controls. Seventy patients had no attack (group 1), 30 had 1-2 attacks (group 2), and 23 had 3 or more attacks (group 3) within last three months. Serum 25(OH)D3 concentrations were determined using liquid chromatography–tandem mass spectrometry. BsmI, FokI, and TaqI polymorphisms were analyzed by a competitive allele specific polymerase chain reaction assay (KASPar). Serum lipid parameters were measured with enzymatic colorimetric methods. 25(OH)D3 concentrations were lower in FMF patients compared to controls (p < 0.001). No difference was observed in 25(OH)D3 concentration between groups 1, 2, and 3. The distributions of FokI and TaqI genotypes were not significantly different between FMF patients and controls. There was a significant difference in the distribution of AA BsmI genotype between male FMF patients and male controls. Increased concentrations of triglycerides (p = 0.012) and decreased concentrations of high-density lipoprotein cholesterol [HDL-C] (p = 0.006) were found in FMF patients compared to controls. Although lower 25(OH)D3 concentrations were observed in FMF patients versus controls, no association was determined between FMF attack frequency and 25(OH)D3 concentrations. We showed that the AA genotype of BsmI polymorphism is associated with FMF in males but not in females. The effects of decreased HDL-C and increased triglyceride concentrations on cardiovascular events in FMF patients should be further investigated

Hekimlerin Ölümle Karşılaşma Sıklıklarına Gore Ötenazi Harkındaki Görüşlerinin Değerlendirilmesi

Giriş Ötenazi, tedavisi mümkün olmayan ve insanda acıma duygusu uyandıran bir rahatsızlığı bulunan kişinin acılarını dindirmek için hastanın talebiyle ya da iradesinin alına­madığı hallerde kanuni temsilcisi veya mirasçıla­rının izni ile icrai ya da ihmali bir davranışla, tıbbi yoldan hastanın hayatına son verilmesidir. Dünyada bir çok ülkede olduğu gibi ülkemizde de ötenazinin her şekli suç olarak sayılmaktadır. Bu çalışmada; terminal dönemde durumu kötü olan veya sonu ölümcül olabilecek derecede hastalığı olan hastalarla ilgilenen hekimler ile sonu ölümcül derecede hastalıkları olmayanlar ile ilgilenen hekimlerin ötenazi hakkındaki görüşlerinin karşılaştırılmasını kapsamaktadır.   Materyal ve Metod Anket çalışmamız 2013 yılı içerisinde Dicle ve Gaziantep Üniversiteleri Tıp Fakültesi Hastanelerinde Temel Bilimler Bölümlerinde, hemodiyaliz, yoğun bakım ünitelerinde, hematoloji ve onkoloji servislerinde, dahiliye, pediatri, psikiyatri ve fizik tedavi kliniklerinde görev yapan hekimlere uygulanmıştır. 169 olgu cinsiyet, yaş, medeni durum, çocuk sayısı, yaşadıkları aile tipi, çalıştıkları bölüm, ölümle karşılaşma sıklıkları, mesleki deneyimi, yatağa bağımlı bir yakınları olup-olmaması, kendilerine ve yakınlarına ötenazi isteyip-istememe görüşlerine göre incelenmiştir.   Bulgular Olguların çalıştıkları bölümler genel durumu ağır ve mortalitesi yüksek olan hastalarla karşılaşma sıklığına göre 3 alt grupta değerlendirilmiştir. Ankete katılanların 60 (%35.5)’ı kadın ve 109 (%64.5)’u erkektir. Olgularımızdan 105’i (%62.1) evli, 63'ü (%37.3) bekar, 1'i (%0.6) dul olduğunu belirtmiştir.  Ankete katılanların 85 (%50.3)’i yaşına ve tanısına bağlı olarak ötenaziyi düşünebileceğini, 20 (%11.8) olgu kararsız olduklarını ve 64 (%37.9) olguda hangi durumda olursa olsun düşünmeyeceklerini belirtmişledir.   Tartışma ve Sonuç Ötenazi, tıbbi ve hukuki yönüne ek olarak insanların inancını ve duygu durumlarını da içeren bir hadisedir. Her ne kadar ülkemizde ötenazi yasak olsa da hekimlerin hiçte azımsanmayacak bir kısmı ülkemizde pasif ötenazinin uygulandığı kanaatindedir. Ötenazi ister pasif ister aktif bir şekilde uygulansın bu durum sağlık çalışanları, hasta ve hasta yakınları için çok zor bir karardır. Anahtar Kelimeler: Ötenazi, ölüm, heki