Prevalence of Arcobacter, Escherichia coli, Staphylococcus aureus and Salmonella species in Retail Raw Chicken, Pork, Beef and Goat meat in Osogbo, Nigeria

Three hundred (300) samples of fresh raw chicken, beef, goat and pork meat were screened for Arcobacter species by selective cultural procedures and for Escherichia coli, Salmonella species and Staphylococcus aureus enriched in peptone water and then streaked onto appropriate bacteriological agar. From the 300 samples analysed, S. aureus {138 (46%)} was the most frequently isolated organism, followed by E. coli {78 (26%)}, Arcobacter spp. {57(19%)} and Salmonella spp {6(2%)}. In this study, varying level of resistance of Escherichia coli 66(84.6%), Salmonella 6(100%) and Arcobacter 57(100%) to amoxicillin was observed. The susceptibility pattern indicates that the bacterial isolates exhibited a varying level of resistance to two or more antimicrobial agents with maximum resistance to amoxicillin. The detection of these organisms in meat may constitute a serious public health concern. Hence, there is a need for the implementation of Hazard Analysis Critical Control Point System monitoring of critical contamination points used in meat production to ensure food safety in Nigeria. Keywords: Abattoir-hygiene, Antibiotic resistance, Foodborne-pathogens, Nigeri

A study of microbial analysis of fresh fruits and vegetables, in Sagamu markets South-West, Nigeria

Raw and minimally processed fruits and vegetables are essential parts of diets of people around the world. However, these food items have consistently served as vehicles for human diseases worldwide. This study was aimed at determining the microbiological quality of fresh fruits and vegetables sold at Sagamu markets, Ogun state, Nigeria. Twenty different samples of fruits and vegetables were randomly purchased from ten various vendors in Shagamu market, Ogun State, Nigeria. Aerobic plate counts, total coliform counts, yeast, mold counts and antimicrobial susceptibility study using were determined using standard microbiological methods. In Fruits, the isolation rate of Bacillus specie was predominant (44.1%), followed by Klebsiella species (44.0%) while in vegetables, the prevalent rates of 38.3% and 14.4% were respectively recorded by Staphylococcus aureus and Pseudomonas species. Malassezia species and Aspergillus species were the only fungal isolates obtained from the fruits and vegetables. Aspergillus species were predominant in vegetables, with a frequency of 13.3% while both Malassezia and Aspergillus species were observed almost at the same frequently of 24.1% and 24.4% in fruits respectively. This study showed that fruits and vegetable were contaminated with potential food borne pathogens with varying degree of antibiotic resistance thus suggesting that they could act as a vehicle for the dissemination of antibiotic resistant organisms as well as serve as a reservoir of food borne pathogens of public health significance.Keywords: Micro flora, fresh fruits and vegetables, antibiotics resistanc

Comparative effects of washing solution and the survival of Staphylococcus aureus on tomatoes

Various types of fruits and vegetables are often eaten raw or consumed after minimum processing in which this has been connected to an increase in outbreaks of fresh produce associated with food borne diseases in the recent times. In order to determine the micro-flora, survival of Staphylococcus aureus on ripe and unripe tomatoes and to also asses the efficiency of some washing solutions, 100 tomatoes, both ripe and unripe were randomly bought from different selling points in Sagamu Markets, Ogun State Nigeria. Surface swabs of the tomatoes were cultured on both nutrient broth, and selenite F broth. The sample from nutrient broth was then sub cultured on Blood Agar, Mannitol Salt Agar, and MacConkey Agar, (MSA), Potato Dextrose Agar (SDA), while the one on Selenite F Broth was cultured on Salmonella Shigella Agar (SSA). Density of Staphylococcus aureus ATCC 25923 after pre-inoculation on ripe and unripe tomatoes, and after the application of washing solutions on tomatoes, was determined by surface spread count. The prevalence of any of the isolated micro-flora could not be significantly linked with either ripe or unripe tomatoes (0>0.05).Mean residual counts (log CFU/g) of bacteria was significantly higher in ripe tomatoes (3.52 ±2.40) than unripe tomatoes (2.30 ±1.96) after pre inoculation with Staphylococcus aureus (t =2.75, P< 0.05). Among the washing solution, hypochlorite was observed to be twice more efficient than hydrogen peroxide and thrice efficient than saline solution. In conclusion, Staphylococcal colonization of tomatoes skin is significantly reduced in unripe tomatoes. And also, hypochlorite solutions are better than hydrogen peroxide and saline in Staphylococcal decontamination from tomatoes.Key words: washing solution, Staphylococcus aureus, tomatoe

Prevalence of intestinal parasitic infections among HIV patients in Benin City, Nigeria

This study was carried out to determine the presence of intestinal parasites and their correlation with CD4+ T-cell counts and demographics among human immunodeficiency virus (HIV)-positive patients in Benin City, Nigeria. Stool specimens from 2,000 HIV-positive patients and 500 controls (HIV-negative individuals) were examined for ova, cysts, or parasites, using standard procedures. In addition, patient's blood samples were analyzed for CD4 counts by flow cytometry. An overall prevalence rate of 15.3% was observed among HIV-positive patients while 6.2% was noted among non-HIV subjects. HIV status was a significant (P<0.0001) risk factor for acquiring intestinal parasitic infections. Male gender, CD4 count <200cell/µl, and diarrhea were significantly associated with an increased prevalence of intestinal parasitic infections among HIV-positive patients. The level of education, occupation, and source of water among HIV patients significantly (P<0.0001) affected the prevalence of intestinal parasitic infections. Ascaris lumbricoides was the most predominant parasite in both HIV-positive patients and controls. A CD4 count <200 cells/µl was significantly associated with only Isospora belli and Cryptosporidium infections. The presence of pathogenic intestinal parasites such as A. lumbricoides, hookworm, Giardia intestinalis, Entamoeba histolytica, Trichuris trichiura, and Taenia species among HIV-infected persons should not be neglected. Cryptosporidium species and I. belli were the opportunistic parasites observed in this study. Routine screening for intestinal parasites in HIV-positive patients is advocated

Epidemiology of bacterial zoonoses in Nigeria

This paper dicusses the epidemiology and epizootiology of bacterial zoonotic diseases in Nigeria. Six diseases are discussed including anthrax, brucellosis, bovine tuberculosis, campylobacteriosis leptospirosis, salmonellosis and tetanus. All axcept anthrax are enzootic and endemic in the country, and have been associated with high morbidity in human and the animal populations. The exact impact of the diseases on the general population in unknown because they are grossly underreported and underrecognized. Keywords: epidemiology, bacteria, zoonoses, endemic, enzootic Nigerian Journal of Health and Biomedical Sciences Vol. 5(1) 2006: 98-10

Impact of emmerging zoonosis and public health significance in Nigeria

Emerging zoonotic diseases have assumed an increasing importance as a result of its negative impacts on the local, national and international human and economic policies in public and animal health care delivery services. Rapid detection of emerging diseases as well as precise effective actions against these diseases is slow in many developing countries. Morbidity and mortality due to zoonosis are mostly recorded due to deficiencies in the veterinary infrastructure, expertise, diagnostic laboratories and in surveillance capabilities. This review discusses updates on global impact of zoonosis, an evaluation of major zoonotic agents with particular reference to situation in Nigeria considering the effects of the farming systems of sub-Saharan Africa, as well as the attitudes and activities of the people as reported in the literature were also discussed. This review was carried out using internet search machines to evaluate the current situation through a review of data and publications devoted to the global control of zoonosis. Contributing factors that continue to affect emerging and re-emerging zoonosis in Nigeria include social, political and economic factors. Since zoonotic diseases are peculiar, the preparedness and response capability of a country towards an emerging disease largely depend on the availability of such facilities, therefore, their prevention and control will require unique strategies, based more on fundamental research than on traditional approaches thus necessitating a collaborative approach of local, national and international agencies working in human, animal health, food and other health practitioner communities are warranted, along with improved education of the general public and policy makers.Key words: Emerging, zoonoses, factors, Nigeria, public healt

Arcobacter Adherence, Invasion And Cytotoxic Effect On Human Carcinoma Cell

No Abstract