Investigation of Association between Slime Production by <i>Candida Spp</i> and Susceptibility to Fluconazole and Voriconazole

Purpose: To determine the susceptibilities of fluconazole and voriconazole based on slime production by Candida spp. Methods: Candida strains (115) isolated in the period between January 2011 and January 2012 were included in this study. Conventional methods were used for the identification. Candida albicans and non-C. albicans isolates were tested for slime production with modified tube adherence test and antifungal resistance with disk diffusion method. Results: Slime positivity was 31.3 % in all Candida species. Slime positivity in non-C.albicans isolates (44.89 %) was higher than in C. albicans species (21.21 %). All C. albicans isolates were sensitive to fluconazole and voriconazole. The highest resistance to fluconazole (40 %) and voriconazole (5%) was by C. glabrata strains. Conclusion: Species definition and determination of antifungal susceptibility patterns are advised for the proper management and treatment of patients

Rhamnolipid Biosurfactants Produced by Pseudomonas Species

ABSTRACT: Surfactants are chemical products widely used in our daily life in toothpaste and other personal hygiene and cosmetic products, and in several industries. Biosurfactants are surfactants of biological origin that can be produced by microorganisms and have many advantages, such as low toxicity and high biodegradability, compared to synthetic counterparts. Unfortunately, high production costs limit the use of biosurfactants. Low-cost production is the most important factor for biosurfactants to be able to compete in the global market place. This review presents general information on rhamnolipid biosurfactant produced by Pseudomonas species, as well as on their production and applications. In addition, industrial products and their wastes used for rhamnolipid production are reviewed in detail based on recent studies

Investigation of Association between Slime Production by Candida Spp and Susceptibility to Fluconazole and Voriconazole

Purpose: To determine the susceptibilities of fluconazole and voriconazole based on slime production by Candida spp. Methods: Candida strains (115) isolated in the period between January 2011 and January 2012 were included in this study. Conventional methods were used for the identification. Candida albicans and non-C. albicans isolates were tested for slime production with modified tube adherence test and antifungal resistance with disk diffusion method. Results: Slime positivity was 31.3 % in all Candida species. Slime positivity in non-C.albicans isolates (44.89 %) was higher than in C. albicans species (21.21 %). All C. albicans isolates were sensitive to fluconazole and voriconazole. The highest resistance to fluconazole (40 %) and voriconazole (5%) was by C. glabrata strains. Conclusion: Species definition and determination of antifungal susceptibility patterns are advised for the proper management and treatment of patients

Synthesis and potent antistaphylococcal activity of some new 2-[4-(3,4-dimethoxyphenoxy) phenyl]-1, N-disubstituted-1H-benzimidazole-5-carboxamidines

A series of new 2-[4-(3,4-dimethoxyphenoxy) phenyl]-1, N-disubstituted-1H-benzimidazole-5carboxamidines (23-33) have been synthesized and evaluated for their potential antistaphylococcal activity. Cytotoxic effects of the compounds were investigated by the neutral red uptake (NRU) cytotoxicity test. Most of the compounds exhibited good MICs values against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). Compound 28 with N-cyclohexylcarboxamidine group at the 5-position was found to be the most potent agent, with the MIC value of 3.12 mu g/mL

Synthesis and In Vitro Activity of Polyhalogenated 2-phenylbenzimidazoles as a New Class of anti-MRSA and Anti-VRE Agents

A series of novel polyhalogenated 2-phenylbenzimidazoles have been synthesized and evaluated for in vitro antistaphylococcal activity against drug-resistant bacterial strains (methicillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococcus faecium. Certain compounds inhibit bacterial growth perfectly. 11 was active than vancomycin (0.78 mu g/mL) with the lowest MIC values with 0.19 mu g/mL against methicillin-resistant Staphylococcus aureus, 8 and 35 exhibited best inhibitory activity against vancomycin-resistant Enterococcus faecium (1.56 mu g/mL). The mechanism of action for this class of compounds appears to be different than clinically used antibiotics. These polyhalogenated benzimidazoles have potential for further investigation as a new class of potent anti-methicillin-resistant Staphylococcus aureus and anti-vancomycin-resistant Enterococcus faecium agents