The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples

Introduction: Some studies of prostate cancer (PCa) have reported the presence of human papillomavirus (HPV) DNA. Polymorphisms in IL-10 gene can influence inflammation and immune response and may be related to the risk of prostate cancer. The capacity for IL-10 production varies according to the genetic composition of the IL-10 locus. We aimed to elucidate the relation between HPV infection and IL-10 polymorphism for the development of prostate cancer. We examined 108 formalin-fixed specimens for the existence of HPVs DNA and IL-10 -1082 genotype distribution. Materials and Methods: The DNA are extracted from archival prostate tissues of totally 108 patients, 40 of whom with adenocarcinoma and 68 with benign prostatic hyperplasia (BPH). Genotypes of IL-10 -1082 single nucleotide polymorphism (SNP) were performed using allele specific polymerase chain reaction (ARMS). HPV detection was performed by using conventional HPV primers. Results: HPVs DNA was detected in 15 of 68 BPH specimens (22%) and in 16 of 40 prostate PCa specimens (40%). Distribution of IL-10 -1082 genotype was not statistically different between PCa and BPH using chi-square (p> 0.05). There was not any association between HPVs DNA positivity and IL-10 -1082 genotypes. Conclusion: The results suggest that the HPVs DNA positivity might be involved in the etiology of a minority of prostate cancers. As result, we consider that future investigations are needed to provide conclusive evidence on the role of this pathogen and genes in the prostate cancer

Investigation of Cytomegalovirus, Epstein-Barr Virus and Adenovirus Infections After Hematopoietic Stem Cell Transplantation (HSCT) in Pediatric Patients

WOS: 000458960500003Introduction: Due to immunosuppressive treatments, viral infections, especially cytomegalovirus (CMV), Epstein-Barr virus (EBV) and adenovirus (ADV) infections, are the main causes of morbidity and mortality in patients receiving hematopoietic stem cell transplantation (HSCT). The aim of this study was to investigate the incidence of CMV, EBV and ADV infections in pediatric patients who had HSCT. Materials and Methods: Between November 2015 and November 2016, 51 patients between the ages of 16 months and 16 years having had HSCT were included into the study. Forty-six patients received allogeneic and 5 patients autologous HSCT. Quantitative real-time PCR (Anatolia Geneworks, Turkey) test was used to detect CMV, EBV and ADV viral load in blood. Results: Of the 46 patients receiving allogeneic HSCT as a single infection or co-infection, viral load above 1000 copies/mL was found in 26% (12) for CMV, in 26% (12) for EBV and 2.1% (1) for ADV. Twenty (43.4%) allogeneic HSCT recipients was put under preemptive therapy and viremia was cleared in 19. Only 1 (2.1%) patient died due to EBV infection and acute graft versus host disease (GVHD). Conclusion: Early diagnosis by monitoring viral load and initiation of preemptive treatment is important to prevent complications of viral infections in patients after HSCT