Metabolic Pathways Enhancement Confers Poor Prognosis in p53 Exon Mutant Hepatocellular Carcinoma.

RNA-Sequencing (RNA-Seq), the most commonly used sequencing application tool, is not only a method for measuring gene expression but also an excellent media to detect important structural variants such as single nucleotide variants (SNVs), insertion/deletion (Indels), or fusion transcripts. The Cancer Genome Atlas (TCGA) contains genomic data from a variety of cancer types and also provides the raw data generated by TCGA consortium. p53 is among the top 10 somatic mutations associated with hepatocellular carcinoma (HCC). The aim of the present study was to analyze concordant different gene profiles and the priori defined set of genes based on p53 mutation status in HCC using RNA-Seq data. In the study, expression profile of 11 799 genes on 42 paired tumor and adjacent normal tissues was collected, processed, and further stratified by the mutated versus normal p53 expression. Furthermore, we used a knowledge-based approach Gene Set Enrichment Analysis (GSEA) to compare between normal and p53 mutation gene expression profiles. The statistical significance (nominal P value) of the enrichment score (ES) genes was calculated. The ranked gene list that reflects differential expression between p53 wild-type and mutant genotypes was then mapped to metabolic process by KEGG, an encyclopedia of genes and genomes to assign functional meanings. These approaches enable us to identify pathways and potential target gene/pathways that are highly expressed in p53 mutated HCC. Our analysis revealed 2 genes, the hexokinase 2 (HK2) and Enolase 1 (ENO1), were conspicuous of red pixel in the heatmap. To further explore the role of these genes in HCC, the overall survival plots by Kaplan-Meier method were performed for HK2 and ENO1 that revealed high HK2 and ENO1 expression in patients with HCC have poor prognosis. These results suggested that these glycolysis genes are associated with mutated-p53 in HCC that may contribute to poor prognosis. In this proof-of-concept study, we proposed an approach for identifying novel potential therapeutic targets in human HCC with mutated p53. These approaches can take advantage of the massive next-generation sequencing (NGS) data generated worldwide and make more out of it by exploring new potential therapeutic targets

An hourglass model for the flare of HST-1 in M87

To explain the multi-wavelength light curves (from radio to X-ray) of HST-1 in the M87 jet, we propose an hourglass model that is a modified two-zone system of Tavecchio & Ghisellini (hereafter TG08): a slow hourglass-shaped or Laval nozzle-shaped layer connected by two revolving exponential surfaces surrounding a fast spine, through which plasma blobs flow. Based on the conservation of magnetic flux, the magnetic field changes along the axis of the hourglass. We adopt the result of TG08---the high-energy emission from GeV to TeV can be produced through inverse Compton by the two-zone system, and the photons from radio to X-ray are mainly radiated by the fast inner zone system. Here, we only discuss the light curves of the fast inner blob from radio to X-ray. When a compressible blob travels down the axis of the first bulb in the hourglass, because of magnetic flux conservation, its cross section experiences an adiabatic compression process, which results in particle acceleration and the brightening of HST-1. When the blob moves into the second bulb of the hourglass, because of magnetic flux conservation, the dimming of the knot occurs along with an adiabatic expansion of its cross section. A similar broken exponential function could fit the TeV peaks in M87, which may imply a correlation between the TeV flares of M87 and the light curves from radio to X-ray in HST-1. The Very Large Array (VLA) 22 GHz radio light curve of HST-1 verifies our prediction based on the model fit to the main peak of the VLA 15 GHz radio light curve.Comment: 14 pages, 2 figures, accepted for publication in A

Acceptance Of Wireless Internet Via Mobile Technology In China

This study explores factors associated with the acceptance of Wireless Internet via Mobile Technology (WIMT) in China. The results indicate that the acceptance of WIMT is related to the factors of perceived usefulness, perceived ease of use, social influences, wireless trust environment, and facilitating conditions. It provides diagnostic insight into how different factors influence user intention to accept WIMT in China, and thus help business develop strategy to prompt WIMT communications and mobile commerce there

Study on the extraction method of transverse open crack’s information

For the fault rotor – bearing system caused by transverse open crack. The dynamic model of crack rotor system is established by the crack compliance coefficient matrix which is derived from the stress intensity factor and strain energy density function. The stiffness matrix of rotor system which contains transverse crack faults is different from the health rotor. So the surplus dynamics equation of cracked rotor system can be deduced by comparing the dynamics equations of the crack fault and health rotor system, which is on the basis of getting the compliance coefficient matrix. Furthermore, the information of open crack’s location and crack’s depth can be extracted from the vibration signal by analyzing force condition on both ends of the shaft segment where crack exist and combining with the residual dynamic equation. The extraction method for crack information only needs to collect the vibration signals of the three different node positions under two different speeds. Finally, the feasibility of the method can be verified with simulation and experiment

Experimental Investigation of Oxide Leaching Methods for Li Isotopes

To examine the applicability of different leaching methods used to extract secondary oxides from silicate solids for lithium isotope (δ7Li) analysis, this study has conducted leaching experiments on five different types of silicate solids, including a fresh basalt, two weathered basalts, a Yellow River sediment (loess-dominated) and a shale. Four factors were assessed in the experiments: the concentration of the leaching reagent hydroxylamine hydrochloride (HH), the leaching temperature (20 °C versus 95 °C), the leaching time and the reagent/solid ratio. Based on elemental concentrations and Li isotopes, 0.04 mol l-1 hydroxylamine hydrochloride (HH) in 25% v/v acetic acid at room temperature for 1 hour with 40 ml g-1 reagent/solid ratio is recommended. At high temperature, low δ7Li and high magnesium/iron ratios indicate that minerals other than secondary oxides are dissolved. With increased leaching time, there is no evidence for Li isotopic fractionation at room temperature. However, longer leaching time or increased reagent/solid ratios may increase the risk of leaching from non-oxide phases. Meanwhile, results suggest that low concentrations of HH are not sufficient to target the secondary oxides evenly, while high concentrations of HH can leach out more non-oxides. We also examined the optimal oxide leaching method within a full sequential leaching procedure (i.e., exchangeable, carbonate, oxide, clay and residual phases). Elemental concentrations show that no elements exist exclusively in oxides, so it is essential to analyse multi-elemental concentrations to verify that the leaching has accessed this phase in a given sample. Comparing secondary oxides with their corresponding solutions, we estimate the isotopic fractionation (Δ7Lioxide-solution) is -16.8‰ to -27.7‰

Observation of Majorana fermions with spin selective Andreev reflection in the vortex of topological superconductor

Majorana fermion (MF) whose antiparticle is itself has been predicted in condensed matter systems. Signatures of the MFs have been reported as zero energy modes in various systems. More definitive evidences are highly desired to verify the existence of the MF. Very recently, theory has predicted MFs to induce spin selective Andreev reflection (SSAR), a novel magnetic property which can be used to detect the MFs. Here we report the first observation of the SSAR from MFs inside vortices in Bi2Te3/NbSe2 hetero-structure, in which topological superconductivity was previously established. By using spin-polarized scanning tunneling microscopy/spectroscopy (STM/STS), we show that the zero-bias peak of the tunneling differential conductance at the vortex center is substantially higher when the tip polarization and the external magnetic field are parallel than anti-parallel to each other. Such strong spin dependence of the tunneling is absent away from the vortex center, or in a conventional superconductor. The observed spin dependent tunneling effect is a direct evidence for the SSAR from MFs, fully consistent with theoretical analyses. Our work provides definitive evidences of MFs and will stimulate the MFs research on their novel physical properties, hence a step towards their statistics and application in quantum computing.Comment: 4 figures 15 page