Trajectory analysis of Polar Patrol Balloon (PPB) flights in the stratosphere over Antarctica in summer and spring: A preliminary result

Actual trajectories of two PPB's which flew in the Antarctic stratosphere in austral summer and spring are compared with those calculated based on objective analysis data of Japan Meteorological Agency (JMA). The differences between the actual and calculated trajectories are discussed to check reliability of the JMA objective analysis data for the stratosphere, and to detect subsynoptic scale variability due to gravity waves and others

Evaluation of drinking ease relative to the opening diameter and beverage type of aluminum beverage bottles

金沢大学理工研究域機械工学系This paper investigates the effects of different bottle opening sizes (28, 33, and 38 mm in diameter) and beverage types (e.g. green tea, carbonated beverages) on the drinking satisfaction of consumers, in order to enhance their comfort levels when drinking from aluminum beverage bottles. A survey of 120 Japanese subjects was conducted, and the 33 mm opening size emerged as the most preferred, irrespective of beverage type. The factor analysis results of the questionnaire show that drinking satisfaction is primarily affected by two common factors: the volume of flow from the bottle to the mouth and the adjustability of the flow. The results of the three-dimensional fluid-dynamics analysis indicate that differences in beverage type could influence what consumers regard as an appropriate flow. © 2009 Elsevier Ltd. All rights reserved

Finite element analysis applied to ergonomic design of 2-piece aluminum beverage cans and bottles

金沢大学理工研究域機械工学系This paper has introduced the finite element analysis (FEA) into the ergonomie design to evaluate the human feelings numerically and objectively, and then into the optimization design of beverage containers considering human factors. In the design of the end of can (the lid of can), experiments and the FEA of indenting vertically the fingertip pulp by a probe and the tab of end have been done to observe force responses and to study feelings in the fingertip. A numerical simulation of finger lifting the tab for opening the can has also been performed, and discomfort in the fingertip has been evaluated numerically to present the finger-accessibility of the tab. The comparison of finger-accessibility between two kinds of tab ring shape designs showed that the tab that may have a larger contact area with the finger is better. In the design of beverage bottles served hot drinks, the FEA of tactile sensation of heat has been performed to evaluate numerically the touch feeling of the finger when holding the hot bottle. The numerical simulations of embossing process have also been performed to evaluate the formability of various rib-shape designs. The optimum design has then been done considering the hot touch feeling as well as the metal sheet formability. Copyright © 2007 by ASME

Evaluation method of drinking ease for aluminum beverage bottles

金沢大学理工研究域機械工学系This paper has investigated effects of the bottle opening size on drinking feelings in order to improve the comfort level of consumers when drinking directly from the opening of aluminum bottle. A survey over 120 subjects has been performed based on a drinking test using three kinds of bottles with opening diameters of 28, 33 and 38 mm, respectively. Two questionnaires have been conducted. Statistical analysis results of Questionnaire 1 have shown that 33-mm opening is best for adult consumers with no matter the type of contents, gender and the mouth size. The factor analysis results of Questionnaire 2 based on Kansei Engineering have shown that drinking feeling is affected by two common factors, which considered as the flow from the bottle to the mouth and the flow adjustability. Moreover, the fluid-dynamics analysis model has been developed to simulate the bottled liquid in a drinking action consisting of survey results and experimental observations of consumers\u27 drinking actions. Numerical simulations have been performed to understand how consumers control the flow during the drinking actions. It is found that the consumers usually try to realize the ideal and preferable condition by adjusting the inclination angle of the bottle. Copyright © 2007 by ASME

Newly Identified CYP2C93 Is a Functional Enzyme in Rhesus Monkey, but Not in Cynomolgus Monkey

Cynomolgus monkey and rhesus monkey are used in drug metabolism studies due to their evolutionary closeness and physiological resemblance to human. In cynomolgus monkey, we previously identified cytochrome P450 (P450 or CYP) 2C76 that does not have a human ortholog and is partly responsible for species differences in drug metabolism between cynomolgus monkey and human. In this study, we report characterization of CYP2C93 cDNA newly identified in cynomolgus monkey and rhesus monkey. The CYP2C93 cDNA contained an open reading frame of 490 amino acids approximately 84–86% identical to human CYP2Cs. CYP2C93 was located in the genomic region, which corresponded to the intergenic region in the human genome, indicating that CYP2C93 does not correspond to any human genes. CYP2C93 mRNA was expressed predominantly in the liver among 10 tissues analyzed. The CYP2C93 proteins heterologously expressed in Escherichia coli metabolized human CYP2C substrates, diclofenac, flurbiprofen, paclitaxel, S-mephenytoin, and tolbutamide. In addition to a normal transcript (SV1), an aberrantly spliced transcript (SV2) lacking exon 2 was identified, which did not give rise to a functional protein due to frameshift and a premature termination codon. Mini gene assay revealed that the genetic variant IVS2-1G>T at the splice site of intron 1, at least partly, accounted for the exon-2 skipping; therefore, this genotype would influence CYP2C93-mediated drug metabolism. SV1 was expressed in 6 of 11 rhesus monkeys and 1 of 8 cynomolgus monkeys, but the SV1 in the cynomolgus monkey was nonfunctional due to a rare null genotype (c.102T>del). These results suggest that CYP2C93 can play roles as a drug-metabolizing enzyme in rhesus monkeys (not in cynomolgus monkeys), although its relative contribution to drug metabolism has yet to be validated

Thinning minimization for forming aluminum beverage can end shells

Forming simulations of the can end shell have been implemented based on both of the axisymmetric model and three-dimensional models, for a better understanding of the forming process. The comparison shows that the simulation results agree reasonably well with the experimental observations of the actual forming process. The influence of the loads applied to tools, the clearance between tools, the shape of the tool profile and the position of tools have been investigated, based on the axisymmetric model to save computational time. The design optimization method based on the numerical simulations have been applied to search the optimum design points, in order to reduce the thinning subjected to the constraints of the geometric shape of the shell and the suppression of wrinkles. The optimization results show that the thinning can be improved up to 4% by optimizing the forming route, adjusting the clearance and the load, and modifying the tool shape. © 2010 by the American Institute of Aeronautics and Astronautics, Inc. All rights reserved

Oral Exposure to Polystyrene Microplastics of Mice on a Normal or High-Fat Diet and Intestinal and Metabolic Outcomes

マイクロプラスチックの経口摂取が高脂肪食条件下での代謝障害を悪化させる. 京都大学プレスリリース. 2023-02-24.[Background:] Microplastics (MPs) are small particles of plastic (≤ 5mm in diameter). In recent years, oral exposure to MPs in living organisms has been a cause of concern. Leaky gut syndrome (LGS), associated with a high-fat diet (HFD) in mice, can increase the entry of foreign substances into the body through the intestinal mucosa. [Objectives:] We aimed to evaluate the pathophysiology of intestinal outcomes associated with consuming a high-fat diet and simultaneous intake of MPs, focusing on endocrine and metabolic systems. [Methods:] C57BL6/J mice were fed a normal diet (ND) or HFD with or without polystyrene MP for 4 wk to investigate differences in glucose tolerance, intestinal permeability, gut microbiota, as well as metabolites in serum, feces, and liver. [Results:] In comparison with HFD mice, mice fed the HFD with MPs had higher blood glucose, serum lipid concentrations, and nonalcoholic fatty liver disease (NAFLD) activity scores. Permeability and goblet cell count of the small intestine (SI) in HFD-fed mice were higher and lower, respectively, than in ND-fed mice. There was no obvious difference in the number of inflammatory cells in the SI lamina propria between mice fed the ND and mice fed the ND with MP, but there were more inflammatory cells and fewer anti-inflammatory cells in mice fed the HFD with MPs in comparison with mice fed the HFD without MPs. The expression of genes related to inflammation, long-chain fatty acid transporter, and Na⁺/glucose cotransporter was significantly higher in mice fed the HFD with MPs than in mice fed the HFD without MPs. Furthermore, the genus Desulfovibrio was significantly more abundant in the intestines of mice fed the HFD with MPs in comparison with mice fed the HFD without MPs. Muc2 gene expression was decreased when palmitic acid and microplastics were added to the murine intestinal epithelial cell line MODE-K cells, and Muc2 gene expression was increased when IL-22 was added. [Discussion:] Our findings suggest that in this study, MP induced metabolic disturbances, such as diabetes and NAFLD, only in mice fed a high-fat diet. These findings suggest that LGS might have been triggered by HFD, causing MPs to be deposited in the intestinal mucosa, resulting in inflammation of the intestinal mucosal intrinsic layer and thereby altering nutrient absorption. These results highlight the need for reducing oral exposure to MPs through remedial environmental measures to improve metabolic disturbance under high-fat diet conditions

Complications Associated With Spine Surgery in Patients Aged 80 Years or Older: Japan Association of Spine Surgeons with Ambition (JASA) Multicenter Study

Study Design:Retrospective study of registry data.Objectives:Aging of society and recent advances in surgical techniques and general anesthesia have increased the demand for spinal surgery in elderly patients. Many complications have been described in elderly patients, but a multicenter study of perioperative complications in spinal surgery in patients aged 80 years or older has not been reported. Therefore, the goal of the study was to analyze complications associated with spine surgery in patients aged 80 years or older with cervical, thoracic, or lumbar lesions.Methods:A multicenter study was performed in patients aged 80 years or older who underwent 262 spinal surgeries at 35 facilities. The frequency and severity of complications were examined for perioperative complications, including intraoperative and postoperative complications, and for major postoperative complications that were potentially life threatening, required reoperation in the perioperative period, or left a permanent injury.Results:Perioperative complications occurred in 75 of the 262 surgeries (29%) and 33 were major complications (13%). In multivariate logistic regression, age over 85 years (hazard ratio [HR] = 1.007, P = 0.025) and estimated blood loss ≥500 g (HR = 3.076, P = .004) were significantly associated with perioperative complications, and an operative time ≥180 min (HR = 2.78, P = .007) was significantly associated with major complications.Conclusions:Elderly patients aged 80 years or older with comorbidities are at higher risk for complications. Increased surgical invasion, and particularly a long operative time, can cause serious complications that may be life threatening. Therefore, careful decisions are required with regard to the surgical indication and procedure in elderly patients