Biospecimen Reporting for Improved Study Quality

Human biospecimens are subject to a number of different collection, processing, and storage factors that can significantly alter their molecular composition and consistency. These biospecimen preanalytical factors, in turn, influence experimental outcomes and the ability to reproduce scientific results. Currently, the extent and type of information specific to the biospecimen preanalytical conditions reported in scientific publications and regulatory submissions varies widely. To improve the quality of research utilizing human tissues, it is critical that information regarding the handling of biospecimens be reported in a thorough, accurate, and standardized manner. The Biospecimen Reporting for Improved Study Quality recommendations outlined herein are intended to apply to any study in which human biospecimens are used. The purpose of reporting these details is to supply others, from researchers to regulators, with more consistent and standardized information to better evaluate, interpret, compare, and reproduce the experimental results. The Biospecimen Reporting for Improved Study Quality guidelines are proposed as an important and timely resource tool to strengthen communication and publications around biospecimen-related research and help reassure patient contributors and the advocacy community that the contributions are valued and respected.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90474/1/bio-2E2010-2E0036.pd

Balanço do nitrogênio e fósforo em solo com cultivo orgânico de hortaliças após a incorporação de biomassa de guandu.

Os objetivos deste trabalho foram avaliar os efeitos de faixas de guandu (Cajanus cajan) e da incorporação da biomassa proveniente de sua poda na fertilidade do solo e na produtividade de três hortaliças sob cultivo orgânico. O delineamento usado foi de blocos casualizados completos em esquema de parcelas subsubdivididas com três repetições. As produtividades de beterraba, cenoura e feijão-de-vagem não foram afetadas pelos tratamentos. Nas parcelas onde não houve incorporação da biomassa de guandu, o balanço de nitrogênio no sistema foi negativo, ao passo que com a incorporação, esse balanço foi positivo. Embora tenha ocorrido balanço positivo para o fósforo nas parcelas sem a incorporação de biomassa de guandu, houve um aumento significativo na absorção desse elemento pelas hortaliças quando o material foi incorporado. O sistema de cultivo em aléias de guandu pode representar uma prática vantajosa para os produtores orgânicos, por contribuir na manutenção da fertilidade do solo

The Natural Cytotoxicity Receptor 1 Contribution to Early Clearance of Streptococcus pneumoniae and to Natural Killer-Macrophage Cross Talk

Natural killer (NK) cells serve as a crucial first line of defense against tumors, viral and bacterial infections. We studied the involvement of a principal activating natural killer cell receptor, natural cytotoxicity receptor 1 (NCR1), in the innate immune response to S. pneumoniae infection. Our results demonstrate that the presence of the NCR1 receptor is imperative for the early clearance of S. pneumoniae. We tied the ends in vivo by showing that deficiency in NCR1 resulted in reduced lung NK cell activation and lung IFNγ production at the early stages of S. pneumoniae infection. NCR1 did not mediate direct recognition of S. pneumoniae. Therefore, we studied the involvement of lung macrophages and dendritic cells (DC) as the mediators of NK-expressed NCR1 involvement in response to S. pneumoniae. In vitro, wild type BM-derived macrophages and DC expressed ligands to NCR1 and co-incubation of S. pneumoniae-infected macrophages/DC with NCR1-deficient NK cells resulted in significantly lesser IFNγ levels compared to NCR1-expressing NK cells. In vivo, ablation of lung macrophages and DC was detrimental to the early clearance of S. pneumoniae. NCR1-expressing mice had more potent alveolar macrophages as compared to NCR1-deficient mice. This result correlated with the higher fraction of NCR1-ligandhigh lung macrophages, in NCR1-expressing mice, that had better phagocytic activity compared to NCR1-liganddull macrophages. Overall, our results point to the essential contribution of NK-expressed NCR1 in early response to S. pneumoniae infection and to NCR1-mediated interaction of NK and S. pneumoniae infected-macrophages and -DC

Envisioning the Indigenised university for sustainable development

Purpose: Whilst the possibility of a university fostering sustainable development is present in the extant literature and policy documents, the idea still warrants further consideration. This paper, therefore, aims to identify the nature and outcomes of the university’s engagement with Indigenous communities and perceptions of Indigenous knowledge systems in both academic and non-academic activities, and what might be required to foster the university’s contributions toward sustainable development. Design/methodology/approach: A qualitative case study of the only public university in The Gambia was conducted, including non-university actors. Interviews and focus group discussion methods were employed, and these enabled close collaboration between researchers and participants, and the latter were empowered to describe their perceptions of reality. Findings: Three major sets of findings emerged from the analysis of the transcripts from interviews and focus group discussions with the university and community members. These are the limited nature of and outcomes from university-community engagement, the sustainable outcomes of Indigenous practices, and ideas for Indigenising university engagement for sustainable development. Originality: Matters of the university reaching out to Indigenous peoples have yet to find their way into conceptualisations of the university for sustainable development. This paper addresses this gap in the existing literature by advancing possibilities for the Indigenised university for sustainable development to emerg

Wet and Dry Season Effects on Select Soil Nutrient Contents of Upland Farms in North Bank Region of the Gambia

Abstract The study was conducted in three villages of North Bank Region of the Gambia in 2013 and 2014. We examined wet and dry season effects on select soil nutrient contents of upland farms in North Bank Region of the Gambia. The objective was to evaluate changes in soil nutrient contents in both wet and dry seasons. Soil samples were collected from three RCBD upland fields with three replications at a depth of 0 -15 cm and analyzed for pH, Soil Organic Carbon (SOC), and soil moisture content. The gravimetric method of moisture estimation was used. The results showed that soil moisture content, soil TN, and soil pH are significantly different (P < 0.05) during the two seasons. There was no significant difference in SOC between the two seasons in the study area. The study concluded that soil nutrients were more readily available during the wet season than during the dry season probably because there is more soil moisture available in the wet season that facilitates soil nutrient release. The study concludes that soil moisture has to be available in order for some select soil nutrients to be released for plant uptake