Energy Rationale for the Use of the Thermophilic Mode of Anaerobic Bioconversion of Liquid Organic Waste in the Climatic Conditions of the Russian Federation

The transition of livestock production to industrial processes and the concentration of animals associated with this process on large farms and complexes has caused a sharp increase in the volume of manure that must be disposed of without pollution. One of the ways of processing organic waste (biomass) is its anaerobic digestion in biogas plants through the vital activity of microorganisms (methanogenesis).Biogas obtained using microbiological processing of biomass can be used as a raw material for heat and electric energy. Annually, 0.17% of the total livestock manure produced at Russian agricultural enterprisesis used for biogas production.The main component of a biogas plant is a manure fermentation reactor, the required volume of which is determined by the daily output of manure from the livestock farm, the temperature and the hydraulic retention time of treatment. This research explored thermal energy consumption of biogas plants, using the example of a biogas plant of a modular design that depended on the average annual outdoor temperature. Based on the calculations, the thermophilic mode was found to be more energy-efficient than the mesophilic one; thus, with the thermophilic mode, the specific energy consumption needed for the plant was lower at the average annual outdoor temperatures of all the constituent entities of the Russian Federation. At the same time, the specific biogas yield in the thermophilic regime was 20-50%higher than in the mesophilic regime. Keywords: anaerobic processing, agricultural waste, thermophilicmode, mesophilicmode, energy costs, energy rational

The start-up of continuous biohydrogen production from cheese whey: Comparison of inoculum pretreatment methods and reactors with moving and fixed polyurethane carriers

This article presents the results of the start-up of continuous production of biohydrogen from cheese whey (CW) in an anaerobic filter (AF) and anaerobic fluidized bed (AFB) with a polyurethane carrier. Heat and acid pretreatments were used for the inactivation of hydrogenscavengers in the inoculum (mesophilic and thermophilic anaerobic sludge). Acid pretreatment was effective for thermophilic anaerobic sludge to suppress methanogenic activity, and heat treatment was effective for mesophilic anaerobic sludge. Maximum specific yields of hydrogen, namely 178 mL/g chemical oxygen demand (COD) and 149 mL/g COD for AFB and AF, respectively, were obtained at the hydraulic retention time (HRT) of 4.5 days and organic load rate (OLR) of 6.61 kg COD/(m3 day). At the same time, the maximum hydrogen production rates of 1.28 and 1.9 NL/(L day) for AF and AFB, respectively, were obtained at the HRT of 2.02 days and OLR of 14.88 kg COD/(m3 day). At the phylum level, the dominant taxa were Firmicutes (65% in AF and 60% in AFB), and at the genus level, Lactobacillus (40% in AF and 43% in AFB) and Bifidobacterium (24% in AF and 30% in AFB)

Syntrophic Butyrate-Oxidizing Consortium Mitigates Acetate Inhibition through a Shift from Acetoclastic to Hydrogenotrophic Methanogenesis and Alleviates VFA Stress in Thermophilic Anaerobic Digestion

In anaerobic digestion (AD), butyrate is degraded by syntrophic consortium, but can accumulate in highly loaded AD systems. The effect of butyrate on the AD process attracts much less attention than propionate or acetate. In this work, an enrichment culture of the thermophilic butyrate-oxidizing syntrophic consortium was obtained by gradually increasing the initial butyrate concentration from 20 to 170 mM. Surprisingly, even the highest butyrate concentration did not significantly inhibit the methanogenic community, and the stage of acetate degradation was the limiting overall rate of the process. At 170 mM butyrate, the bacterial community changed towards the dominance of syntrophic acetate-oxidizing (SAO) bacteria related to Syntrophaceticus (42.9%), Syntrophomonas (26.2%) and Firmicutes (26.2%), while the archaeal community experienced a sharp decrease in the abundance of Methanosarcina thermophila (from 86.0 to 25.0%) and increase in Methanothermobacter thermautotrophicus (from 3.2 to 53.1%) and Methanomassiliicoccus (from 3.2 to 21.9%). Thus, the shift from acetoclastic methanogenesis to SAO coupled to hydrogenotrophic methanogenesis occurred as an adaptive strategy to overcome high acetate (~200 mM) build-up. Bioaugmentation with the obtained enrichment culture was effective in mitigating the butyrate-dominated VFA build-up during the AD of readily biodegradable waste, increasing the methane production rate, methane yield and volatile solids removal by more than 3.5, 6.2 and 2.9 times, respectively. Our study revealed that the thermophilic butyrate-oxidizing consortia as bioaugmented culture could be the potential strategy to alleviate the high organic load and VFA stress of AD