Influence of Grassland Management on Carbon Allocation in a Semiarid Temperate Steppe

Grazing lands in North China are often excessively grazed and widely degraded, while hay-making lands appear to be in relatively good condition due to grazing exclusion, but they are facing a continuous loss of nutrients in the harvested biomass. In semiarid grasslands, plant productivity and community composition are significantly altered by grazing and haying. Grazing mostly leads to negative effects on aboveground productivity, however root biomass seems to increase with moderate grazing (Gao et al. 2009; Derner et al. 2006), although responses can vary. Aboveground biomass removal can increase C3 grass dominance and productivity (Hofer and Bragg 1981). Grazing exclusion is a valuable mechanism of sequestering soil C (He 2008). However, grazing can change C allocation patterns and affect the amount of C entering the soil. Here we examine the potential effects of common management practices (exclusion with fencing, grazing and hay-making) on semiarid grasslands above- and below-ground C pools. The primary objective of this study was to evaluate the potential of grazing exclusion and annual last-summer haying in previous grazing lands on the storage of C in semiarid grasslands of northern China

Topological Transitions with an Imaginary Aubry-Andre-Harper Potential

We study one-dimensional lattices with imaginary-valued Aubry-Andre-Harper (AAH) potentials. Such lattices can host edge states with purely imaginary eigenenergies, which differ from the edge states of the Hermitian AAH model and are stabilized by a non-Hermitian particle-hole symmetry. The edge states arise when the period of the imaginary potential is a multiple of four lattice constants. They are topological in origin, and can manifest on domain walls between lattices with different modulation periods and phases, as predicted by a bulk polarization invariant. Interestingly, the edge states persist and remain localized even if the real line gap closes. These features can be used in laser arrays to select topological lasing modes under spatially extended pumping

Computational Analysis of Drought Stress-Associated miRNAs and miRNA Co-Regulation Network in Physcomitrella patens.

miRNAs are non-coding small RNAs that involve diverse biological processes. Until now, little is known about their roles in plant drought resistance. Physcomitrella patens is highly tolerant to drought; however, it is not clear about the basic biology of the traits that contribute P. patens this important character. In this work, we discovered 16 drought stress-associated miRNA (DsAmR) families in P. patens through computational analysis. Due to the possible discrepancy of expression periods and tissue distributions between potential DsAmRs and their targeting genes, and the existence of false positive results in computational identification, the prediction results should be examined with further experimental validation. We also constructed an miRNA co-regulation network, and identified two network hubs, miR902a-5p and miR414, which may play important roles in regulating drought-resistance traits. We distributed our results through an online database named ppt-miRBase, which can be accessed at http://bioinfor.cnu.edu.cn/ppt_miRBase/index.php. Our methods in finding DsAmR and miRNA co-regulation network showed a new direction for identifying miRNA functions

Small-molecule inhibitors of phosphatidylcholine transfer protein/StarD2 identified by high-throughput screening

Phosphatidylcholine transfer protein (PC–TP, also referred to as StarD2) is a highly specific intracellular lipid-binding protein that catalyzes the transfer of phosphatidylcholines between membranes in vitro. Recent studies have suggested that PC–TP in vivo functions to regulate fatty acid and glucose metabolism, possibly via interactions with selected other proteins. To begin to address the relationship between activity in vitro and biological function, we undertook a high-throughput screen to identify small-molecule inhibitors of the phosphatidylcholine transfer activity of PC–TP. After adapting a fluorescence quench assay to measure phosphatidylcholine transfer activity, we screened 114,752 compounds of a small-molecule library. The high-throughput screen identified 14 potential PC–TP inhibitors. Of these, 6 compounds exhibited characteristics consistent with specific inhibition of PC–TP activity, with IC50 values that ranged from 4.1 to 95.0 ?M under conditions of the in vitro assay. These compounds should serve as valuable reagents to elucidate the biological function of PC–TP. Because mice with homozygous disruption of the PC–TP gene (Pctp) are sensitized to insulin action and relatively resistant to the development of atherosclerosis, these inhibitors may also prove to be of value in the management of diabetes and atherosclerotic cardiovascular diseases

Shear stress regulation of miR-93 and miR-484 maturation through nucleolin.

Pulsatile shear (PS) and oscillatory shear (OS) elicit distinct mechanotransduction signals that maintain endothelial homeostasis or induce endothelial dysfunction, respectively. A subset of microRNAs (miRs) in vascular endothelial cells (ECs) are differentially regulated by PS and OS, but the regulation of the miR processing and its implications in EC biology by shear stress are poorly understood. From a systematic in silico analysis for RNA binding proteins that regulate miR processing, we found that nucleolin (NCL) is a major regulator of miR processing in response to OS and essential for the maturation of miR-93 and miR-484 that target mRNAs encoding Krüppel-like factor 2 (KLF2) and endothelial nitric oxide synthase (eNOS). Additionally, anti-miR-93 and anti-miR-484 restore KLF2 and eNOS expression and NO bioavailability in ECs under OS. Analysis of posttranslational modifications of NCL identified that serine 328 (S328) phosphorylation by AMP-activated protein kinase (AMPK) was a major PS-activated event. AMPK phosphorylation of NCL sequesters it in the nucleus, thereby inhibiting miR-93 and miR-484 processing and their subsequent targeting of KLF2 and eNOS mRNA. Elevated levels of miR-93 and miR-484 were found in sera collected from individuals afflicted with coronary artery disease in two cohorts. These findings provide translational relevance of the AMPK-NCL-miR-93/miR-484 axis in miRNA processing in EC health and coronary artery disease

RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair

Unrestrained 53BP1 activity at DNA double-strand breaks (DSBs) hampers DNA end resection and upsets DSB repair pathway choice. RNF169 acts as a molecular rheostat to limit 53BP1 deposition at DSBs, but how this fine balance translates to DSB repair control remains undefined. In striking contrast to 53BP1, ChIP analyses of AsiSI-induced DSBs unveiled that RNF169 exhibits robust accumulation at DNA end-proximal regions and preferentially targets resected, RPA-bound DSBs. Accordingly, we found that RNF169 promotes CtIP-dependent DSB resection and favors homology-mediated DSB repair, and further showed that RNF169 dose-dependently stimulates single-strand annealing repair, in part, by alleviating the 53BP1-imposed barrier to DSB end resection. Our results highlight the interplay of RNF169 with 53BP1 in fine-tuning choice of DSB repair pathways

Two-soliton solution for the derivative nonlinear Schr\"odinger equation with nonvanishing boundary conditions

An explicit two-soliton solution for the derivative nonlinear Schr\"odinger equation with nonvanishing boundary conditions is derived, demonstrating details of interactions between two bright solitons, two dark solitons, as well as one bright soliton and one dark soliton. Shifts of soliton positions due to collisions are analytically obtained, which are irrespective of the bright or dark characters of the participating solitons.Comment: 11 pages, 4 figures. Phys. Lett. A 2006 (in press