Multi-cluster dynamics in Λ13C^{13}_\Lambda{\rm C} and analogy to clustering in 12C^{12}{\rm C}

We investigate structure of $^{13}_\Lambda{\rm C}$ and discuss the difference and similarity between the structures of $^{12}{\rm C}$ and $^{13}_\Lambda{\rm C}$ by answering the questions if the linear-chain and gaslike cluster states, which are proposed to appear in $^{12}{\rm C}$, survives, or new structure states appear or not. We introduce a microscopic cluster model called, Hyper-Tohsaki-Horiuchi-Schuck-R\"opke (H-THSR) wave function, which is an extended version of the THSR wave function so as to describe $\Lambda$ hypernuclei. We obtained two bound states and two resonance (quasi-bound) states for $J^\pi=0^+$ in $^{13}_\Lambda{\rm C}$, corresponding to the four $0^+$ states in $^{12}{\rm C}$. However, the inversion of level ordering between the spectra of $^{12}{\rm C}$ and $^{13}_\Lambda{\rm C}$, i.e. that the $0_3^+$ and $0_4^+$ states in $^{13}_\Lambda{\rm C}$ correspond to the $0_4^+$ and $0_3^+$ states in $^{12}{\rm C}$, respectively, is shown to occur. The additional $\Lambda$ particle reduces sizes of the $0_2^+$ and $0_3^+$ states in $^{13}_\Lambda{\rm C}$ very much, but the shrinkage of the $0_4^+$ state is only a half of the other states. In conclusion, the Hoyle state becomes quite a compact object with ${^{9}_\Lambda{\rm Be}}+\alpha$ configuration in $^{13}_\Lambda{\rm C}$ and is no more gaslike state composed of the $3\alpha$ clusters. Instead, the $0_4^+$ state in $^{13}_\Lambda{\rm C}$, coming from the $^{12}{\rm C}(0_3^+)$ state, appears as a gaslike state composed of $\alpha+\alpha+^{5}_\Lambda{\rm He}$ configuration, i.e. the Hoyle analog state. A linear-chain state in a $\Lambda$ hypernucleus is for the first time predicted to exist as the $0_3^+$ state in $^{13}_\Lambda{\rm C}$ with more shrunk arrangement of the $3\alpha$ clusters along $z$-axis than the $3\alpha$ linear-chain configuration realized in the $^{12}{\rm C}(0_4^+)$ state.Comment: 9 pages, 6 figures, figures rearranged, accepted for publication in PL

Modélisation des conditions d’environnement des bocaux de culture in vitro : bocaux avec agar et vitroplants

La présente étude traite de la modélisation des conditions des plantes en culture in vitro. Lorsque des vitroplants sont introduits dans les bocaux,l'humidité de l'air intérieur varie en fonction de leur stade de développementet ce en accord avec les courbes expérimentales. Les variations diurnes du déficit de pression de vapeur (DPV) présentent un maximum pendant laphotopériode et un minimum pendant la nyctipériode. Par ailleurs, grâce à la prise en compte des réservoirs de la plante, le modèle permet de décrire la déshydratation et la réhydratation des tissus, ce qui est potentiellement d'un intérêt pratique. Au cours du cycle diurne, la teneur en eau relative varie entre 75 et 90 %. Les flux d'eau dans la plante concordent bien avec les variations de la teneur en eau du réservoir plante et caractérisent bien les deux types de régimes de circulation de l'eau dans la plante : le régime de flux conservatif et le régime de flux non conservatif.Mots-clés : Modélisation, culture in vitro, humidité de l'air intérieur, réservoir-plante, déshydratation, réhydratatio

Modeling the microclimate inside a vessel in in vitro culture : vessel with agar

Numerical simulations show that variations in vessel internal humidity was sensitive to transfer coefficient, climatic conditions within the growth chamber, evaporation and condensation of water vapor on the walls of the vessel. The variations in water vapor pressure deficits (VPD) (low during the nyctiperiod and high during the photoperiod) were well explained by a free convection model, which took into account temperature differences. Finally, the results show the necessity to construct containers with variable openings and growth chambers in which bioclimatic conditions can be controlled.Les simulations numériques révèlent que les variations de l\'humidité interne ont été sensibles au coefficient d\'échange de vapeur d\'eau, aux conditions climatiques de la chambre de culture et aux phénomènes d\'évaporation et de condensation de vapeur d\'eau sur les parois des bocaux. Par ailleurs, il a été établi que le «créneau» de déficit de pression de vapeur (DPV) de l\'air intérieur (palier de DPV faible en nyctipériode et palier de DPV élevé en photopériode) a été bien imité en mode convectif libre qui tient compte des écarts de températures. Ainsi, ces résultats montrent la nécessité de construire des récipients à ouverture variable et des chambres de culture dans lesquelles les facteurs bioclimatiques des plantes peuvent être maîtrisés. Keywords: Agar, in vitro culture, microclimate, modeling, transfer coefficient./Agar, culture in vitro, microclimat, modélisation, coefficient d\'échange.Sciences & Nature Vol. 4 (1) 2007: pp. 75-8

A case of wound dual infection with Pasteurella dagmatis and Pasteurella Canis resulting from a dog bite - limitations of Vitek-2 system in exact identification of Pasteurella species

<p>Abstract</p> <p>Background</p> <p><it>Pasteurella </it>species, widely known as indigenous orgganisms in the oral and gastrointestinal floras of many wild and domestic animals, are important pathogens in both animals and humans. Human infections due to <it>Pasteurella </it>species are in most cases associated with infected injuries following animal bites. We encountered a rare case of dual infections caused by different two <it>Pasteurella </it>species occurred in a previously healthy 25-year-old female sustaining injury by a dog-bite.</p> <p>Methodology</p> <p>Exudates from the open wound of her dog-bite site, together with the saliva of the dog were submitted for bacteriological examination. Predominantly appearing grayish-white smooth colonies with almost the same colonial properties but slightly different glistening grown on chocolate and sheep blood agar plates were characterized morphologically by Gram's stain, biochemically by automated instrument using Vitek 2 system using GN cards together with commercially available kit system, ID-Test HN-20 rapid panels, and genetically by sequencing the 16S rRNA genes of the organism using a Taq DyeDeoxy Terminator Cycle Sequencing and a model 3100 DNA sequencer instrument.</p> <p>Results</p> <p>The causative isolates from the dog-bite site were finally identified as <it>P</it>. <it>canis </it>and <it>P</it>. <it>dagmatis </it>from the findings of the morphological, cultural, and biochemical properties together with the comparative sequences of the 16S rRNA genes. Both the isolates were highly susceptible to many antibiotics and the patient was successfully treated with the administration of so-called the first generation cephalosporin, cefazolin followed by so-called the third generation cephalosporin, cefcapene pivoxil. The isolate from the dog was subsequently identified as <it>P</it>. <it>canis</it>, the same species as the isolate from the patient.</p> <p>Conclusions</p> <p>To the best of our knowledge, this was the second report of a dual infection with <it>Pasteurella </it>species consisting of <it>P</it>. <it>dagmatis </it>and <it>P. canis </it>resulting from a dog-bite, followed by the first report of dual infections due to <it>P</it>. <it>dagmatis </it>and <it>P. multocida </it>in 1988. Our isolate finally identified as <it>P</it>. <it>dagmatis </it>was misidentified as <it>P</it>. <it>pneumotripica by </it>means of the Vitek 2 system. The species name "<it>P</it>. <it>dagmatis" </it>was not included in the database of the system. It is also important for routine clinical microbiology laboratories to know the limitation of the automated Vitek 2 system for the accurate identification of <it>Pasteurella </it>species especially <it>P</it>. <it>dagmatis</it>. It should be emphasized that there still exists much room for improvement in Vitek 2 system. Significant improvement of Vitek 2 system especially in the identification of <it>Pasteurella </it>species is urgently desired.</p

Clinical significance of VEGF-A, -C and -D expression in esophageal malignancies

Vascular endothelial growth factors ( VEGF)- A, - C and - D are members of the proangiogenic VEGF family of glycoproteins. VEGF-A is known to be the most important angiogenic factor under physiological and pathological conditions, while VEGF-C and VEGF-D are implicated in the development and sprouting of lymphatic vessels, so called lymphangiogenesis. Local tumor progression, lymph node metastases and hematogenous tumor spread are important prognostic factors for esophageal carcinoma ( EC), one of the most lethal malignancies throughout the world. We found solid evidence in the literature that VEGF expression contributes to tumor angiogenesis, tumor progression and lymph node metastasis in esophageal squamous cell carcinoma ( SCC), and many authors could show a prognostic value for VEGF-assessment. In adenocarcinoma (AC) of the esophagus angiogenic properties are acquired in early stages, particularly in precancerous lesions like Barrett's dysplasia. However, VEGF expression fails to give prognostic information in AC of the esophagus. VEGF-C and VEGF-D were detected in SCC and dysplastic lesions, but not in normal mucosa of the esophagus. VEGF-C expression might be associated with lymphatic tumor invasion, lymph node metastases and advanced disease in esophageal SCC and AC. Therapeutic interference with VEGF signaling may prove to be a promising way of anti-angiogenic co-treatment in esophageal carcinoma. However, concrete clinical data are still pending

Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos

hybridization. hybridization (WISH).Here we describe a modification to the WISH protocol that is essential to prevent DNA cross-hybridization and to specifically detect transgene mRNA transcripts in electroporated embryos. Our optimized WISH procedure can be applied not only to electroporated chick embryos but also to other embryos or adult tissues that have been transfected with large amounts of reporter- or expression construct DNA

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B

Changes in selected haematological parameters associated with JAK1/JAK2 inhibition observed in patients with rheumatoid arthritis treated with baricitinib

Objective To characterise changes in selected haematological parameters following once-daily oral baricitinib dosing.Methods Data were pooled from eight randomised clinical trials (four phase 3, three phase 2, one phase 1b) and one long-term extension. Changes in haematological parameters were evaluated up to 128 weeks (N=2387); overall safety of baricitinib was assessed up to 6 years (N=3492).Results Mean absolute neutrophil counts decreased (-1.36x10(9)/L) within 1 month, followed by stabilisation within the normal reference range through week 128. The incidence of serious infections was not elevated in patients with neutropenia during the 24-week placebo-controlled period. Mean lymphocyte counts increased (+0.30x10(9)/L) within 1 month, then decreased to baseline (weeks 12-24). Mean platelet counts increased at week 2 (+51x10(9)/L), then decreased towards baseline. Overall, mean haemoglobin concentrations decreased (-0.12 mmol/L), then returned to baseline; however, reduced baseline haemoglobin concentrations observed in the highest baseline high-sensitivity C reactive protein quartile increased over time. Permanent drug discontinuation occurred due to laboratory abnormalities related to neutrophil count in 8 (0.2%), lymphocyte counts in 6 (0.2%), platelet counts in 8 (0.2%), and haemoglobin levels in 16 (0.5%) of all baricitinib-treated patients (N=3492 with 7993 total person-years of exposure).Conclusions Moderate decreases in neutrophils were seen during baricitinib treatment; however, serious infection was uncommon in patients with neutropenia. Transient increases were observed in lymphocytes and platelets, which returned to baseline over time. Changes in haemoglobin concentration were generally small. Haematological abnormalities seldom led to drug discontinuation.Pathophysiology and treatment of rheumatic disease