Analisa Algoritma Haversine Formula Untuk Pencarian Lokasi Terdekat Rumah Sakit Dan Puskesmas Provinsi Gorontalo

Pemerintah Provinsi Gorontalo saat ini dihadapkan pada suatu masalah yang berhubungan dengan layanan informasi data. Data layanan informasi yang berkaitan dengan data sarana puskesmas dan rumah sakit belum terinci, sehingga pemerintah kesulitan dalam pengambilan keputusan dalam bentuk peta digital sehingga kebanyakan masyarakat Gorontalo apabila mengalami masalah kesehatan seperti sakit, kecelakaan, meninggal dan lain-lain, akan sering mengalami kesulitan dalam mencari lokasi terdekat layanan kesehatan. Kegunaaan dari Algoritma Haversine Formula adalah digunakan untuk menghitung jarak antara dua titik di bumi berdasarkan panjang garis lurus antar dua titik tanpa mengabaikan kelengkungan yang dimiliki bumi. Berdasarkan hasil analisa Algoritma Haversine Formula dapat menghitung jarak antara lokasi setiap rumah sakit dan puskesmas yang ada di Provinsi Gorontalo dan berdasarkan jarak tersebut maka masyarakat dapat mengetahui jarak lokasi terdekat antara rumah sakit ke rumah sakit lainnya, begitu juga dengan puskesmas ke puskesmas lainnya

Endonuclease activities of MutLα and its homologs in DNA mismatch repair.

MutLα is a key component of the DNA mismatch repair system in eukaryotes. The DNA mismatch repair system has several genetic stabilization functions. Of these functions, DNA mismatch repair is the major one. The loss of MutLα abolishes DNA mismatch repair, thereby predisposing humans to cancer. MutLα has an endonuclease activity that is required for DNA mismatch repair. The endonuclease activity of MutLα depends on the DQHA(X)2E(X)4E motif which is a part of the active site of the nuclease. This motif is also present in many bacterial MutL and eukaryotic MutLγ proteins, DNA mismatch repair system factors that are homologous to MutLα. Recent studies have shown that yeast MutLγ and several MutL proteins containing the DQHA(X)2E(X)4E motif possess endonuclease activities. Here, we review the endonuclease activities of MutLα and its homologs in the context of DNA mismatch repair

PENGARUH TEMPERATUR UDARA MASUK PADA KARBURATOR TERHADAP UNJUK KERJA MESIN SEPEDA MOTOR HONDA GL MAX

Pada umumnya kendaraan bermotor beroperasi pada pagi hari, siang hari dan malam hari dengan cuaca atau temperatur udara yang tentunya berbeda-beda juga, dimana tekanan udara lebih rendah dari tekanan standard temperatur udara lebih tinggi dari temperatur udara standar begitupun sebaliknya, dengan demikian maka kinerja motor juga akan lebih rendah dari prestasi standar. Dalam Penelitian ini mengkaji pengaruh  temperatur  udara  terhadap kinerja mesin untuk mengetagui seberapa besar pengaruh temperatur udara yang dipanaskan dan temperatur udara tanpa pemanasan terhadap kinerja mesin yaitu : torsi (Te), daya (Ne) dan konsumsi bahan bakar (SFCe). Hasil penelitian menunjukan bahwa temperatur udara masuk karburator  sangat  berpengaruh terhadap unjuk kerja mesin. Temperatur udara dalam hal ini berpengaruh terhadap daya mesin yang dihasilkan Maksimum diperoleh pada variasi temperatur 55 ºC dengan putaran mesin 3500 rpm sebesar 0,474 HP, konsumsi bahan bakar minimum terjasi pada variasi temperatur 27 ºC dengan putaran mesin mesin 3500 rpm sebesar 0,29 Kg/jam  serta effisiensi thermal efektif maksimum terjadi pada variasi temperatur 27 ºC dengan putaran mesin 3500 rpm sebesar 20,02% dan effisiensi thermal indikasi maksimum terjadi pada variasi temperatur 27 ºC dengan putaran mesin 3500 rpm sebesar25,02 %.   Kata kunci : Temperatur Udara, Bahan Bakar, Daya Mesim, Motor Bakar Mensi

Globalization and unemployment in Pakistan

This study analyzes the impact of globalization on unemployment in Pakistan, using annual data for the period 1980 to 2013. Using the ARDL econometric framework, we find that the economic, political and social aspects of globalisation differ in their effects. The data suggest that political and social integration, whilst beneficial in the short run, increase the long run expected unemployment rate. Economic integration appears to be only marginally beneficial in the short run; it is significantly beneficial in the long run but cointegration with the other aspects of globalisation means that it cannot fully counteract their undesirable long-run effect

Evidence that the DNA mismatch repair system removes 1-nucleotide Okazaki fragment flaps.

The DNA mismatch repair (MMR) system plays a major role in promoting genome stability and suppressing carcinogenesis. In this work, we investigated whether the MMR system is involved in Okazaki fragment maturation. We found that in the yeast Saccharomyces cerevisiae, the MMR system and the flap endonuclease Rad27 act in overlapping pathways that protect the nuclear genome from 1-bp insertions. In addition, we determined that purified yeast and human MutSα proteins recognize 1-nucleotide DNA and RNA flaps. In reconstituted human systems, MutSα, proliferating cell nuclear antigen, and replication factor C activate MutLα endonuclease to remove the flaps. ATPase and endonuclease mutants of MutLα are defective in the flap removal. These results suggest that the MMR system contributes to the removal of 1-nucleotide Okazaki fragment flaps

DNA mismatch repair interacts with CAF-1- and ASF1A-H3-H4-dependent histone (H3-H4)2 tetramer deposition.

DNA mismatch repair (MMR) is required for the maintenance of genome stability and protection of humans from several types of cancer. Human MMR occurs in the chromatin environment, but little is known about the interactions between MMR and the chromatin environment. Previous research has suggested that MMR coincides with replication-coupled assembly of the newly synthesized DNA into nucleosomes. The first step in replication-coupled nucleosome assembly is CAF-1-dependent histone (H3-H4)2 tetramer deposition, a process that involves ASF1A-H3-H4 complex. In this work, we used reconstituted human systems to investigate interactions between MMR and CAF-1- and ASF1A-H3-H4-dependent histone (H3-H4)2 tetramer deposition. We have found that MutSα inhibits CAF-1- and ASF1A-H3-H4-dependent packaging of a DNA mismatch into a tetrasome. This finding supports the idea that MMR occurs before the DNA mismatch is packaged into the tetrasome. Our experiments have also revealed that CAF-1- and ASF1A-H3-H4-dependent deposition of the histone (H3-H4)2 tetramers does not interfere with MMR reactions. In addition, we have established that unnecessary degradation of the discontinuous strand that takes place in both DNA polymerase δ (Pol δ)- and DNA polymerase ϵ (Pol ϵ)-dependent MMR reactions is suppressed by CAF-1- and ASF1A-H3-H4-dependent deposition of the histone (H3-H4)2 tetramers. These data suggest that CAF-1- and ASF1A-H3-H4-dependent deposition of the histone (H3-H4)2 tetramers is compatible with MMR and protects the discontinuous daughter strands from unnecessary degradation by MMR machinery