Molecular cloning of full-length coding sequences and characterization of α chains for donkey (Equus asinus) type I collagen

Donkey (Equus asinus) is a good donor for the collagen production. However, the information on mRNA and protein of donkey collagen has never been reported. In this work, the cDNA sequences coding proα1 and proα2 chains of donkey type I procollagen were determined from six and seven overlapping RT-PCR products, respectively. Further characterization of deduced amino acid sequences detailed the propeptides, telopeptides and triple-helical regions in donkey type I procollagen and collagen chains. Two proα chains of donkey type I procollagen share high similarities with corresponding sequences in mammalian species observed in this study. Considering the significance of lysine and proline in the structure and function of collagen, the distribution patterns of these two characteristic residues in α chains of donkey type I collagen were observed. The mRNA expression levels of type I collagen in donkey tissues were evaluated by quantitative real-time PCR.Keywords: Collagen, Col1a1, Col1a2, donkey, complementary DNAAfrican Journal of Biotechnology Vol. 12(27), pp. 4290-430

The weld seam detection method based on the infocrnet model

Weld seam detection is particularly crucial within industrial systems, as it promptly reflects the quality of product fabrication and performance, ensuring the final metallurgical product’s quality while mitigating potential losses stemming from product quality issues. This paper proposed the InfoFPN Cross Refinement Network (InfoCRNet) for the weld seam detection, achieving an accuracy rate of 98,90% and F1 score of 98,85 %

Multi-Objective Optimization of Transonic Compressor Blade Using Evolutionary Algorithm

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77106/1/AIAA-14667-294.pd

Cloning and characterization of a putative transcription factor induced by abiotic stress in Zea mays

bZIP transcription factors have been reported to play important roles in plant responses to abiotic stresses. Here, we reported the cloning and characterization of a putative bZIP transcription factor (ZmbZIP17) from maize inbred line Han21, which is up-regulated by drought treatment. The open reading frame sequence of ZmbZIP17 was obtained by using 5’RACE and RT-PCR. Sequence analysis showed that ZmbZIP17 encodes a polypeptide of 563 amino acids with predicted molecular mass of59.8 kDa and pI of 5.6. Southern blot analysis showed that ZmbZIP17 exists as a single copy gene in maize genome. Subcellular localization of ZmbZIP17 was identified in nucleus. The results of real-time PCR analysis indicated that ZmbZIP17 was up-regulated by drought, heat, ABA and NaCl stressimmediately, which suggested that ZmbZIP17 is an early stage responsive gene to various abiotic stresses. The result also showed that ZmbZIP17 expressed much higher in leaves than in other organs in maize seedlings

Direct evidence of N aggregation and diffusion in Au+Au+ irradiated GaN

A surface amorphized layer and a buried disordered structure were created in gallium nitride (GaN) irradiated using 1.0 MeV1.0MeV Au+Au+ ions to fluences of 25 and 70 Au+/nm270Au+∕nm2 at room temperature. Bubbles of N2N2 gas within both the amorphized and disordered GaN are formed. A gradient profile with a lower N concentration in the amorphized region is observed, which provides direct evidence of N loss by diffusion in the Au+Au+ irradiated GaN. These results are important to understanding the amorphization processes in GaN and may have significant implications for the design and fabrication of GaN-based devices.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87811/2/021903_1.pd

Lamellar carbon nitride membrane for enhanced ion sieving and water desalination

Membrane-based water treatment processes offer possibility to alleviate the water scarcity dilemma in energy-efficient and sustainable ways, this has been exemplified in filtration membranes assembled from two-dimensional (2D) materials for water desalination purposes. Most representatives however tend to swell or disintegrate in a hydrated state, making precise ionic or molecular sieving a tough challenge. Here we report that the chemically robust 2D carbon nitride can be activated using aluminum polycations as pillars to modulate the interlayer spacing of the conjugated framework, the noncovalent interaction concomitantly affords a well-interlinked lamellar structure, to be carefully distinguished from random stacking patterns in conventional carbon nitride membranes. The conformally packed membrane is characterized by adaptive subnanochannel and structure integrity to allow excellent swelling resistance, and breaks permeability-selectivity trade-off limit in forward osmosis due to progressively regulated transport passage, achieving high salt rejection (>99.5%) and water flux (6 L m−2h−1), along with tunable permeation behavior that enables water gating in acidic and alkaline environments. These findings position carbon nitride a rising building block to functionally expand the 2D membrane library for applications in water desalination and purification scenarios

Aerostructural Optimization of a Transonic Compressor Rotor

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77121/1/AIAA-15397-760.pd