Freshwater Mussels as Biological Indicators

Viral hemorrhagic septicemia virus (VHSV) is a devastating rhabdovirus affecting freshwater fishes worldwide. In 2005, a new genotype of VHSV (IVb) was discovered in Lake St. Clair and has consequently spread throughout the Laurentian Great Lakes. While it is widely known that freshwater fishes are hosts and transmitters of VHSV, little is known about the ability of invertebrates to take up and carry the virus. Our (Vera Kazaniwskyj, Y. Zhang, G. Thomas Watters, Dr. Kody Kuehnl, and Barbara Wolfe) objective in this study was to investigate the ability of freshwater mussels to accumulate and transmit VHSV by using two commonly occurring freshwater mussel species (Corbicula fluminea and Amblema plicata) and to assess the efficacy of freshwater mussels as bioindicators of viral presence. Experiments used inoculations of 100 and 200 Tissue Culture Infective Dose units of VHSV. Mussel tissues were tested for VHS at 72h, 120h, and 168h post-exposure using rt-PCR. Initial results indicate that freshwater mussels have the ability to harbor the VHS virus, especially when inoculated with high doses, and thus can serve as valuable indicators of viral presence. These results also indicate mussels are not likely to maintain VHS long term within their tissues, and are therefore not likely vectors of the disease.https://fuse.franklin.edu/forum-2013/1021/thumbnail.jp

Stokes tomography of radio pulsar magnetospheres. II. Millisecond pulsars

The radio polarization characteristics of millisecond pulsars (MSPs) differ significantly from those of non-recycled pulsars. In particular, the position angle (PA) swings of many MSPs deviate from the S-shape predicted by the rotating vector model, even after relativistic aberration is accounted for, indicating that they have non-dipolar magnetic geometries, likely due to a history of accretion. Stokes tomography uses phase portraits of the Stokes parameters as a diagnostic tool to infer a pulsar's magnetic geometry and orientation. This paper applies Stokes tomography to MSPs, generalizing the technique to handle interpulse emission. We present an atlas of look-up tables for the Stokes phase portraits and PA swings of MSPs with current-modified dipole fields, filled core and hollow cone beams, and two empirical linear polarization models. We compare our look-up tables to data from 15 MSPs and find that the Stokes phase portraits for a current-modified dipole approximately match several MSPs whose PA swings are flat or irregular and cannot be reconciled with the standard axisymmetric rotating vector model. PSR J1939+2134 and PSR J0437$-$4715 are modelled in detail. The data from PSR J1939+2134 at 0.61\,GHz can be fitted well with a current-modified dipole at $(\alpha, i) = (22 \pm 2^\circ, 80 \pm 1^\circ)$ and emission altitude 0.4 $r_\text{LC}$. The fit is less accurate for PSR J1939+2134 at 1.414\,GHz, and for PSR J0437$-$4715 at 1.44\,GHz, indicating that these objects may have a more complicated magnetic field geometry, such as a localized surface anomaly or a polar magnetic mountain.Comment: 38 pages, 33 figures, accepted for publication by MNRA

Stokes tomography of radio pulsar magnetospheres. I. Linear polarization

Polarimetric studies of pulsar radio emission traditionally concentrate on how the Stokes vector (I, Q, U, V) varies with pulse longitude, with special emphasis on the position angle (PA) swing of the linearly polarized component. The interpretation of the PA swing in terms of the rotating vector model is limited by the assumption of an axisymmetric magnetic field and the degeneracy of the output with respect to the orientation and magnetic geometry of the pulsar; different combinations of the latter two properties can produce similar PA swings. This paper introduces Stokes phase portraits as a supplementary diagnostic tool with which the orientation and magnetic geometry can be inferred more accurately. The Stokes phase portraits feature unique patterns in the I-Q, I-U, and Q-U planes, whose shapes depend sensitively on the magnetic geometry, inclination angle, beam and polarization patterns, and emission altitude. We construct look-up tables of Stokes phase portraits and PA swings for pure and current-modified dipole fields, filled core and hollow cone beams, and two empirical linear polarization models, L/I = \cos \theta_0 and L/I = \sin \theta_0, where \theta_0 is the colatitude of the emission point. We compare our look-up tables to the measured phase portraits of 24 pulsars in the European Pulsar Network online database. We find evidence in 60% of the objects that the radio emission region may depart significantly from low altitudes, even when the PA swing is S-shaped and/or the pulse-width-period relation is well satisfied. On the other hand, the data are explained adequately if the emission altitude exceeds ~10% of the light cylinder radius. We conclude that Stokes phase portraits should be analysed concurrently with the PA swing and pulse profiles in future when interpreting radio pulsar polarization data.Comment: 60 pages, 58 figures, submitted to MNRAS, accepted 13 Oct 201

An introduction to Graph Data Management

A graph database is a database where the data structures for the schema and/or instances are modeled as a (labeled)(directed) graph or generalizations of it, and where querying is expressed by graph-oriented operations and type constructors. In this article we present the basic notions of graph databases, give an historical overview of its main development, and study the main current systems that implement them

Migration experiences, employment status and psychological distress among Somali immigrants: a mixed-method international study

Background: The discourse about mental health problems among migrants and refugees tends to focus on adverse pre-migration experiences; there is less investigation of the environmental conditions in which refugee migrants live, and the contrasts between these situations in different countries. This cross-national study of two samples of Somali refugees living in London (UK) and Minneapolis, Minnesota, (USA) helps to fill a gap in the literature, and is unusual in being able to compare information collected in the same way in two cities in different countries. Methods: There were two parts to the study, focus groups to gather in-depth qualitative data and a survey of health status and quantifiable demographic and material factors. Three of the focus groups involved nineteen Somali professionals and five groups included twenty-eight lay Somalis who were living in London and Minneapolis. The quantitative survey was done with 189 Somali respondents, also living in London and Minneapolis. We used the MINI International Neuropsychiatric Interview (MINI) to assess ICD-10 and Results: The overall qualitative and quantitative results suggested that challenges to masculinity, thwarted aspirations, devalued refugee identity, unemployment, legal uncertainties and longer duration of stay in the host country account for poor psychological well-being and psychiatric disorders among this group. Conclusion: The use of a mixed-methods approach in this international study was essential since the quantitative and qualitative data provide different layers and depth of meaning and complement each other to provide a fuller picture of complex and multi-faceted life situations of refugees and asylum seekers. The comparison between the UK and US suggests that greater flexibility of access to labour markets for this refugee group might help to promote opportunities for better integration and mental well-being

Prevention of tracheal cartilage injury with modified Griggs technique during percutaneous tracheostomy - Randomized controlled cadaver study

Introduction: Tracheal stenosis is the most common severe late complication of percutaneous tracheostomy causing significant decrease in quality of life. Applying modified Griggs technique reduced the number of late tracheal stenoses observed in our clinical study. The aim of this study was to investigate the mechanism of this relationship. Materials and methods: Forty-six cadavers were randomized into two groups according to the mode of intervention during 2006-2008. Traditional versus modified Griggs technique was applied in the two groups consequently. Wider incision, surgical preparation, and bidirectional forceps dilation of tracheal wall were applied in modified technique. Injured cartilages were inspected by sight and touch consequently. Age, gender, level of intervention, and number of injured tracheal cartilages were registered. Results: Significantly less frequent tracheal cartilage injury was observed after modified (9%) than original (91%) Griggs technique (p<0.001). A moderate association between cartilage injury and increasing age was observed, whereas the level of intervention (p=0.445) and to gender (p=0.35) was not related to injury. Risk of cartilage injury decreased significantly (OR: 0.0264, 95%, CI: 0.005-0.153) with modified Griggs technique as determined in adjusted logistic regression model. Discussion: Modified Griggs technique decreased the risk of tracheal cartilage injury significantly in our cadaver study. This observation may explain the decreased number of late tracheal stenosis after application of the modified Griggs method. © 2012 Akadémiai Kiadó, Budapes

Emergency and critical care services in Tanzania: a survey of ten hospitals.

While there is a need for good quality care for patients with serious reversible disease in all countries in the world, Emergency and Critical Care tends to be one of the weakest parts of health systems in low-income countries. We assessed the structure and availability of resources for Emergency and Critical Care in Tanzania in order to identify the priorities for improving care in this neglected specialty. Ten hospitals in four regions of Tanzania were assessed using a structured data collection tool. Quality was evaluated with standards developed from the literature and expert opinion. Important deficits were identified in infrastructure, routines and training. Only 30% of the hospitals had an emergency room for adult and paediatric patients. None of the seven district and regional hospitals had a triage area or intensive care unit for adults. Only 40% of the hospitals had formal systems for adult triage and in less than one third were critically ill patients seen by clinicians more than once daily. In 80% of the hospitals there were no staff trained in adult triage or critical care. In contrast, a majority of equipment and drugs necessary for emergency and critical care were available in the hospitals (median 90% and 100% respectively. The referral/private hospitals tended to have a greater overall availability of resources (median 89.7%) than district/regional hospitals (median 70.6). Many of the structures necessary for Emergency and Critical Care are lacking in hospitals in Tanzania. Particular weaknesses are infrastructure, routines and training, whereas the availability of drugs and equipment is generally good. Policies to improve hospital systems for the care of emergency and critically ill patients should be prioritised

Early ART-initiation and longer ART duration reduces HIV-1 proviral DNA levels in children from the CHER trial

Background Reduction of the reservoir of latent HIV-infected cells might increase the possibility of long-term remission in individuals living with HIV. We investigated factors associated with HIV-1 proviral DNA levels in children receiving different antiretroviral therapy (ART) strategies in the children with HIV early antiretroviral therapy (CHER) trial. Methods Infants with HIV  <  12 weeks old with CD4%  ≥  25% were randomized in the CHER trial to early limited ART for 40 or 96 weeks (ART-40 W, ART-96 W), or deferred ART (ART-Def). For ART-Def infants or following ART interruption in ART-40 W/ART-96 W, ART was started/re-started for clinical progression or CD4%  <  25%. In 229 participants, HIV-1 proviral DNA was quantified by PCR from stored peripheral blood mononuclear cells from children who had received  ≥  24 weeks ART and two consecutive undetectable HIV-1 RNA 12–24 weeks apart. HIV-1 proviral DNA was compared between ART-Def and ART-96 W at week 96, and in all arms at week 248. Factors associated with HIV-1 proviral DNA levels were evaluated using linear regression. Findings Longer duration of ART was significantly associated with lower HIV-1 proviral DNA at both 96 (p  =  0.0003) and 248 weeks (p  =  0.0011). Higher total CD8 count at ART initiation was associated with lower HIV-1 proviral DNA at both 96 (p  =  0.0225) and 248 weeks (p  =  0.0398). Week 248 HIV-1 proviral DNA was significantly higher in those with positive HIV-1 serology at week 84 than those with negative serology (p  =  0.0042). Intepretation Longer ART duration is key to HIV-1 proviral DNA reduction. Further understanding is needed of the effects of “immune-attenuation” through early HIV-1 exposure. Funding Wellcome Trust, National Institutes of Health, Medical Research Council