Induction of triacylglycerol production in Chlamydomonas reinhardtii: Comparative analysis of different element regimes

Cataloged from PDF version of article.In this study, impacts of different element absence (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) on element uptake and triacylglycerol production was followed in wild type Chlamydomonas reinhardtii CC-124 strain. Macro- and microelement composition of C. reinhardtii greatly differed under element regimes studied. In particular, heavy metal quotas of the microalgae increased strikingly under zinc supplementation. Growth was suppressed, cell biovolume, carbohydrate, total neutral lipid and triacylglycerol levels increased when microalgae were incubated under these element regimes. Most of the intracellular space was occupied by lipid bodies under all nutrient starvations, as observed by confocal microscopy and transmission electron micrographs. Results suggest that sulfur, magnesium and phosphorus deprivations are superior to nitrogen deprivation for the induction triacylglycerol production in C. reinhardtii. On the other hand, FAME profiles of the nitrogen, sulfur and phosphorus deprived cells were found to meet the requirements of international standards for biodiesel. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved

Antioxidant response of Chlamydomonas reinhardtii grown under different element regimes

Nutrient stress is one of the most favorable ways of increasing neutral lipid and high value-added output production by microalgae. However, little is known about the level of the oxidative damage caused by nutrient stress for obtaining an optimal stress level for maximum production of specific molecules. In this study, the antioxidant response of Chlamydomonas reinhardtii grown under element deprivation (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) was investigated. All element regimes caused a decrease in growth, which was most pronounced under N deprivation. Element deprivation and Zn supplementation caused significant increases in H2O2 and lipid peroxidation levels of C.reinhardtii. Decrease in total chlorophyll level was followed by an increase of total carotenoid levels in C.reinhardtii under N and S deprivation while both increased under N supplementation. Confocal imaging of live cells revealed dramatic changes of cell shape and production of neutral lipid bodies accompanied by a decrease of chlorophyll clusters. Antioxidant capacity of cells decreased under N, S and P deprivation while it increased under N and Zn supplementation. Fluctuation of antioxidant enzyme activities in C.reinhardtii grown under different element regimes refers to different metabolic sources of reactive oxygen species production triggered by a specific element absence or overabundance. © 2015 Japanese Society of Phycology

Systematic studies in the genus Malabaila Hoffm. and closely related genera of the Umbelliferae

SIGLEAvailable from British Library Document Supply Centre-DSC:DXN028260 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

A new species of Silene (Caryophyllaceae) from South Anatolia, Turkey

WOS: 000187573700011Silene doganii A.Duran & Y.Menemen sp. nov. from the Amanos Mountains (C6: Osmaniye-Turkey) is described and illustrated. It is closely related to S. caramanica Boiss. & Heldr., from which it differs mainly in its habit, hairiness, leaf and floral features. (C) 2003 The Linnean Society of London

Baz? Fas Convolvulus L. (Convolvulaceae) Cinsi Türlerinde Polen Çal?şmas?

The pollen grains of nine species of Convolvulus L. from Morocco were studied using scanning electron microscopy and light microscopy. The species belong to three sections and four subsections: sections Convolvulus, Acanthocladi Boiss, and Inermes Boiss, and subsections Convolvulus, Spinescentes Boiss., Oleifolii Peter, and Diffusi Boiss. The pollen grains in the genus examined are all 3-zonocolpate. The pollen is suboblate, oblate-spheroidal, prolate-spheroidal and prolate. The nexine is thinner than the sexine and the tectum is distinctly perforated in all species. The pollen grains show variation within the genus in polar and equatorial axes. colpus length and shape. These variations sometimes correspond with the morphological features of the species and, therefore, can be used to distinguish between some closely related species

Astragalus hamzaoglui (Fabaceae), a new species from the east Black Sea region, Turkey

WOS: 000182150700007Astragalus hamzaoglui O. Ketenoglu & Y. Menemen (Fabaceae), a new species from Turkey is described and illustrated. It belongs to section Hypoglottoidei DC. and resembles A. viciifolius DC. and A. viridissimus Freyn & Sint., from which it differs in the habit, indumentum of stem and leaf, and floral features

Comparative fruit studies in a group of tribe peucedaneae (umbelliferae) (2)

Comparative fruit studies (macro- and micro-morphology and anatomy) have been undertaken in a group of tribe Peucedaneae (Umbelliferae) (Malabaila Hoffm.,Pastinaca L., Heracleum L., Stenotaenia Boiss., Opopanax W.D.J. Koch, andGrafia Rchb.). A multivariate analysis was carried out using Simple Matching Coefficient and UPGMA (unweighted pair group method using arithmetic averages)and WPGMA (weighted pair group method using arithmetic averages) clustering methods. Two types of dorsal mericarp surface and margin sufaces were detected:(1) reticulate with a smooth surface, (2) reticulate with a striate surface. On the dorsal surface of the mericarps, three hair shapes and four hair surface types were seen. These hairs are treated as having systematic significance. For instance, a very short triangular hair with a papillose surface is unique to Pastinaca. The shapes of the mericarp and mericarp apex at the base of stylopodium are significant features for generic delimitation. Malabaila is distinguished by its cordate-obovate mericarp with mericarp apex curved at stylopodium base. Anatomical features, such as the number of the vittae on the dorsal surface, the length of the mericarp wing neck and margin, are significant. The genera Stenotaenia and Opopanax are characterized by having many vittae on the dorsal and commissural surfaces. © 2001 Taylor & Francis Group, LLC

Alchemilla boluensis (Rosaceae), a new species from the west Black Sea region (Bolu, Turkey)

WOS: 000182917200005Alchemilla boluensis from the west Black Sea region of Turkey is described as a species new to science and illustrated. It belongs to the section Alchemilla, subsection Calycanthum and series Elatae, and is closely related to A. amoena, from which it mainly differs in its stem and leaf features

Linaria dumanii (Scrophulariaceae), a new species from south Anatolia, Turkey

WOS: 000177056900001Linaria dumanii A. Duran & Y. Menemen sp. nova is described from the Akseki district in Turkey. It. belongs to the section Pelisserianae Valdes and is related to L. pelisseriana (L.) Miller, from which it mainly differs in its very small flowers

A new species of Dianthus (Caryophyllaceae) from Salt Lake, Central Anatolia, Turkey

WOS: 000166363300006Dianthus aydogduii Y. Menemen & E. Hamzaoglu from Salt Lake, Central Anatolia, Turkey, is described as a species new to science and illustrated