Significance of low energy impact damage on modal parameters of composite beams by design of experiments

This paper presents an experimental study on the effects of multi-site damage on the vibration response of composite beams damaged by low energy impacts around the barely visible impact damage limit (BVID). The variation of the modal parameters with different levels of impact energy and density of damage is studied. Vibration tests have been carried out with both burst random and classical sine dwell excitations in order to compare that which of the methods among Polymax and Half Bandwidth Method is more suitable for damping estimation in the presence of damage. Design of experiments (DOE) performed on the experimental data show that natural frequency is a more sensitive parameter for damage detection than the damping ratio. It also highlighted energy of impact as the factor having a more significant effect on the modal parameters. Half Bandwidth Method is found to be unsuitable for damping estimation in the presence of damage

Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification

The recent outbreak of Zika virus (ZIKV) disease caused an enormous number of infections in Central and South America, and the unusual increase in the number of infants born with microcephaly associated with ZIKV infection aroused global concern. Here, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay using a portable device for the detection of ZIKV. The assay specifically detected ZIKV strains of both Asian and African genotypes without cross-reactivity with other arboviruses, including Dengue and Chikungunya viruses. The assay detected viral RNA at 14.5 TCID50/mL in virus-spiked serum or urine samples within 15?min, although it was slightly less sensitive than reference real time RT-PCR assay. We then evaluated the utility of this assay as a molecular diagnostic test using 90 plasma or serum samples and 99 urine samples collected from 120 suspected cases of arbovirus infection in the states of Paraiba and Pernambuco, Brazil in 2016. The results of this assay were consistent with those of the reference RT-PCR test. This portable RT-LAMP assay was highly specific for ZIKV, and enable rapid diagnosis of the virus infection. Our results provide new insights into ZIKV molecular diagnostics and may improve preparedness for future outbreaks

Genome sequencing reveals Zika virus diversity and spread in the Americas

Although the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects have attracted a great deal of attention, much remains unknown about ZIKV disease epidemiology and ZIKV evolution, in part owing to a lack of genomic data. Here we address this gap in knowledge by using multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analysed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental United States. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of surveillance of viral infections. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those that might be relevant to the effectiveness of diagnostic tests

Experimental study of dual-reciprocating-drilling mechanism using design of experiment approach

Y. Gao et al. proved the feasibility of designing a woodwasp (Sirex Noctilio) inspired drill for Earth and extraterrestrial drilling and boring activities [1]. But before an optimised dual reciprocating drill design can be proposed, it is necessary to better understand the driving factors and the important parameters that influence this mechanism’s performance and, power and force requirements. Indeed the insect’s ovipositor is "optimised", through natural selection, for wood; but the dual reciprocating drill will bore into much different substrates. Here, the numerous parameters that could influence the studied mechanism’s performance are identified and the test bench t

Biodynamics : a bridge between physicians & physicists

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