Electrochemical-based biosensors for microRNA detection: Nanotechnology comes into view

Nanotechnology plays an undeniable significant role in medical sciences, particularly in the field of biomedicine. Development of several diagnostic procedures in medicine has been possible through the beneficial application of nano-materials, among which electrochemical nano-biosensors can be mentioned. They can be employed to quantify various clinical biomarkers in detection, evaluation, and follow up stages of the illnesses. MicroRNAs, a group of regulatory short RNA fragments, added a new dimension to the management and diagnosis of several diseases. Mature miRNAs are single-stranded RNA molecules approximately 22 nucleotides in length, which regulate a vast range of biological functions from cellular proliferation and death to cancer development and progression. Recently, diagnostic value of miRNAs in various diseases has been demonstrated. There are many traditional methods for detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR), microarray technology, nanotechnology-based approaches, and molecular biology tools including miRNA biosensors. In comparison with other techniques, electrochemical nucleic acid biosensor methods exhibit many interesting features, and could play an important role in the future nucleic acid analysis. This review paper provides an overview of some different types of nanotechnology-based biosensors for detection of miRNAs. © 201

A dynamical model of equatorial magnetosonic waves in the inner magnetosphere: a machine learning approach

Equatorial magnetosonic waves (EMS), together with chorus and plasmaspheric hiss, play key roles in the dynamics of energetic electron fluxes in the magnetosphere. Numerical models, developed following a first principles approach, that are used to study the evolution of high energy electron fluxes are mainly based on quasilinear diffusion. The application of such numerical codes requires statistical models for the distribution of key magnetospheric wave modes to estimate the appropriate diffusion coefficients. These waves are generally statistically modeled as a function of spatial location and geomagnetic indices (e.g., AE, Kp, or Dst). This study presents a novel dynamic spatiotemporal model for EMS wave amplitude, developed using the Nonlinear AutoRegressive Moving Average eXogenous machine learning approach. The EMS wave amplitude, measured by the Van Allen Probes, are modeled using the time lags of the solar wind and geomagnetic indices as inputs as well as the location at which the measurement is made. The resulting model performance is assessed on a separate Van Allen Probes data set, where the prediction efficiency was found to be 34.0% and the correlation coefficient was 56.9%. With more training and validation data the performance metrics could potentially be improved, however, it is also possible that the EMS wave distribution is affected by stochastic factors and the performance metrics obtained for this model are close to the potential maximum

Minimally invasive presacral approach for revision of an Axial Lumbar Interbody Fusion rod due to fall-related lumbosacral instability: a case report

<p>Abstract</p> <p>Introduction</p> <p>The purpose of this study was to describe procedural details of a minimally invasive presacral approach for revision of an L5-S1 Axial Lumbar Interbody Fusion rod.</p> <p>Case presentation</p> <p>A 70-year-old Caucasian man presented to our facility with marked thoracolumbar scoliosis, osteoarthritic changes characterized by high-grade osteophytes, and significant intervertebral disc collapse and calcification. Our patient required crutches during ambulation and reported intractable axial and radicular pain. Multi-level reconstruction of L1-4 was accomplished with extreme lateral interbody fusion, although focal lumbosacral symptoms persisted due to disc space collapse at L5-S1.</p> <p>Lumbosacral interbody distraction and stabilization was achieved four weeks later with the Axial Lumbar Interbody Fusion System (TranS1 Inc., Wilmington, NC, USA) and rod implantation via an axial presacral approach.</p> <p>Despite symptom resolution following this procedure, our patient suffered a fall six weeks postoperatively with direct sacral impaction resulting in symptom recurrence and loss of L5-S1 distraction. Following seven months of unsuccessful conservative care, a revision of the Axial Lumbar Interbody Fusion rod was performed that utilized the same presacral approach and used a larger diameter implant. Minimal adhesions were encountered upon presacral re-entry. A precise operative trajectory to the base of the previously implanted rod was achieved using fluoroscopic guidance. Surgical removal of the implant was successful with minimal bone resection required. A larger diameter Axial Lumbar Interbody Fusion rod was then implanted and joint distraction was re-established. The radicular symptoms resolved following revision surgery and our patient was ambulating without assistance on post-operative day one. No adverse events were reported.</p> <p>Conclusions</p> <p>The Axial Lumbar Interbody Fusion distraction rod may be revised and replaced with a larger diameter rod using the same presacral approach.</p

The WEBT Campaign on the Blazar 3C279 in 2006

The quasar 3C279 was the target of an extensive multiwavelength monitoring campaign from January through April 2006, including an optical-IR-radio monitoring campaign by the Whole Earth Blazar Telescope (WEBT) collaboration. In this paper we focus on the results of the WEBT campaign. The source exhibited substantial variability of optical flux and spectral shape, with a characteristic time scale of a few days. The variability patterns throughout the optical BVRI bands were very closely correlated with each other. In intriguing contrast to other (in particular, BL Lac type) blazars, we find a lag of shorter- behind longer-wavelength variability throughout the RVB ranges, with a time delay increasing with increasing frequency. Spectral hardening during flares appears delayed with respect to a rising optical flux. This, in combination with the very steep IR-optical continuum spectral index of ~ 1.5 - 2.0, may indicate a highly oblique magnetic field configuration near the base of the jet. An alternative explanation through a slow (time scale of several days) acceleration mechanism would require an unusually low magnetic field of < 0.2 G, about an order of magnitude lower than inferred from previous analyses of simultaneous SEDs of 3C279 and other FSRQs with similar properties.Comment: Accepted for publication in Ap

Revealing nature of GRB 210205A, ZTF21aaeyldq (AT2021any), and follow-up observations with the 4K×\times4K CCD Imager+3.6m DOT

Optical follow-up observations of optical afterglows of gamma-ray bursts are crucial to probe the geometry of outflows, emission mechanisms, energetics, and burst environments. We performed the follow-up observations of GRB 210205A and ZTF21aaeyldq (AT2021any) using the 3.6m Devasthal Optical Telescope (DOT) around one day after the burst to deeper limits due to the longitudinal advantage of the place. This paper presents our analysis of the two objects using data from other collaborative facilities, i.e., 2.2m Calar Alto Astronomical Observatory (CAHA) and other archival data. Our analysis suggests that GRB 210205A is a potential dark burst once compared with the X-ray afterglow data. Also, comparing results with other known and well-studied dark GRBs samples indicate that the reason for the optical darkness of GRB 210205A could either be intrinsic faintness or a high redshift event. Based on our analysis, we also found that ZTF21aaeyldq is the third known orphan afterglow with a measured redshift except for ZTF20aajnksq (AT2020blt) and ZTF19abvizsw (AT2019pim). The multiwavelength afterglow modelling of ZTF21aaeyldq using the afterglowpy package demands a forward shock model for an ISM-like ambient medium with a rather wider jet opening angle. We determine circumburst density of $n_{0}$ = 0.87 cm$^{-3}$, kinetic energy $E_{k}$ = 3.80 $\times 10^{52}$ erg and the afterglow modelling also indicates that ZTF21aaeyldq is observed on-axis ($\theta_{obs} < \theta_{core}$) and a gamma-ray counterpart was missed by GRBs satellites. Our results emphasize that the 3.6m DOT has a unique capability for deep follow-up observations of similar and other new transients for deeper observations as a part of time-domain astronomy in the future.Comment: Accepted for Special Issue of Journal of Astrophysics and Astronomy, 2022, Astrophysical jets and observational facilities: National perspective, 05 -09 April 2021, ARIES Nainita

Emodin Suppresses Migration and Invasion through the Modulation of CXCR4 Expression in an Orthotopic Model of Human Hepatocellular Carcinoma

10.1371/journal.pone.0057015PLoS ONE83

Effect of central and non-central frequency components on the quality of damage imaging

Accurate image reconstruction of damage through Lamb wave diffraction tomography (LWDT) requires substantial information of scatter field. This can be achieved using transducer network to collect the scatter field data. However, this requires a large number of transducers that creates logistical constraints for the practical applications of the technique. Various methods have been developed to improve the practicability of LWDT. One of the main approaches is to employ data at multiple frequencies within the bandwidth of the excitation signal. The objective of this study is to investigate the performance of using the data at non-central frequencies to reconstruct the damage image using LWDT. This provides an understanding on the influence of data at each individual frequency in the damage image reconstruction.In this paper, a series of numerical case studies with consideration of different damage sizes and shapes are carried out. Different non-central frequencies data is used to reconstruct the damage image. The results show that using the data at different non-central frequencies leads to different qualities of the reconstructed damage images. The quality of these reconstructed damage images are then compared to investigate the information contained of the data at each individual frequency. The study shows that the non-central frequencies data can provide additional information in the damage image reconstruction. Overall, the results of this study provide insights into the influences of the data at different frequencies, which is essential to advance the developments of the LWDT.Gnana Teja Pudipeddi, Ching-Tai Ng, Andrei Kotouso

Effectiveness of life skills training on increasing self-esteem of high school students

AbstractObjective This study designed to investigate effectiveness of training life skills on adolescents’ students. Method This study is a pseudo-experimental study which accomplished on 160 students in Karaj city. Subjects of the study selected randomly from list of students in all of the schools of Karaj; then they divided randomly in two groups. Trained counsellors taught the life skills to students of the study group, and 80 reminder subjects assigned as control group. After educating the training program, subjects administered Cooper Smith self-esteem questionnaire (58-items version). Results Findings of the study indicated that life skills training lead to significant increase of self-esteem in study group in contrast to control group subjects. Conclusion Psycho education and mental health programs such as life skills training could cause to increase the necessary skills in students and decline school and educational problems

Do antibiotic-impregnated shunts in hydrocephalus therapy reduce the risk of infection? An observational study in 258 patients

<p>Abstract</p> <p>Background</p> <p>Shunt infection in hydrocephalus patients is a severe, even life-threatening complication. Antibiotic-impregnated shunts (AIS) have been developed in an attempt to reduce rate of shunt infection. The study was performed to analyze if AIS can diminish the rate of shunt infection. The pathogenic nature of shunt infection in patients with AIS systems and those without antibiotic impregnated shunts (non-AIS) was compared.</p> <p>Methods</p> <p>Over a period of 24 months in the Department of Neurosurgery at University Hospital of Tübingen shunt surgery was performed in 258 patients. In 86 patients AIS systems were implanted. Shunt catheters were commercially impregnated with clindamycin and rifampicin. Analysis of the clinical data included sex, age, classification of hydrocephalus, shunt types and risk factors for shunt infection [age (< 1 year and > 80 years), prematurely born patients, external ventricular drainage, former shunt infection, former systemic infection, disturbance of consciousness, former radiation-/chemotherapy]. Infection rates and underlying bacterial pathogens of patients with AIS were compared to patients with implanted non-AIS systems (172 patients).</p> <p>Results</p> <p>AIS and non-AIS patients did not differ in sex, etiology of hydrocephalus and the shunt type. In the AIS group 72 out of 86 patients had at least one risk factor (83.7 %), compared to 126 patients in the non-AIS group (73.3 %). There was no significant difference between the two groups (p = 0.0629; Fisher's exact test). In patients with no risk factors, only one patient with non-AIS suffered from shunt infection. In patients with one or more risk factors the rate for shunt infection was 7.14 % in patients with non-AIS and 6.94 % in patients with AIS. Former shunt infection (p = 0.0124) was related to higher risk for shunt infection. The use of AIS had therefore no significant advantage (p = 0.8611; multiple logistic regression).</p> <p>Significantly related to a shunt infection was the number of shunt surgeries. 190 interventions in the AIS group (2.21 interventions per patient) and 408 in the non-AIS group (2.37 interventions per patient) had been performed (p = 0.3063; Wilcoxon). There was no shunt infection in the group of patients on whom only one shunt surgery was performed. In patients with at least two shunt surgeries the infection rate was 9%. The infection rate in AIS patients was 5/52 (9.6 %) and in the non-AIS 10/114 (8.77 %), (p = 1.0; Fisher's exact test). Staphylococcus epidermidis was the most frequent pathogen for shunt infection. Fourteen out of 15 infections occurred within the first 6 months of surgery. The most frequent pathogen for shunt infection was S. epidermidis. No toxic or allergic complications were seen using the AIS shunt systems. The presented data show a remarkably low infection rate of 5.8 % in the non-AIS group compared to other studies which demonstrated a significant decrease in the infection rate by AIS.</p> <p>Conclusion</p> <p>AIS did not significantly reduce shunt infection in hydrocephalus patients in the presented study. In the AIS group three patients suffered from shunt infections caused by skin ulceration or neurosurgical procedures with exposure of the cerebrospinal liquor after shunt implantation. AIS was not developed to prevent infection in such cases, therefore an advantage of AIS can not be excluded. In view of the presented data and the small number of reported studies a prospective randomized multicenter study is required.</p

Assessing the Privacy Benefits of Domain Name Encryption

As Internet users have become more savvy about the potential for their Internet communication to be observed, the use of network traffic encryption technologies (e.g., HTTPS/TLS) is on the rise. However, even when encryption is enabled, users leak information about the domains they visit via DNS queries and via the Server Name Indication (SNI) extension of TLS. Two recent proposals to ameliorate this issue are DNS over HTTPS/TLS (DoH/DoT) and Encrypted SNI (ESNI). In this paper we aim to assess the privacy benefits of these proposals by considering the relationship between hostnames and IP addresses, the latter of which are still exposed. We perform DNS queries from nine vantage points around the globe to characterize this relationship. We quantify the privacy gain offered by ESNI for different hosting and CDN providers using two different metrics, the k-anonymity degree due to co-hosting and the dynamics of IP address changes. We find that 20% of the domains studied will not gain any privacy benefit since they have a one-to-one mapping between their hostname and IP address. On the other hand, 30% will gain a significant privacy benefit with a k value greater than 100, since these domains are co-hosted with more than 100 other domains. Domains whose visitors' privacy will meaningfully improve are far less popular, while for popular domains the benefit is not significant. Analyzing the dynamics of IP addresses of long-lived domains, we find that only 7.7% of them change their hosting IP addresses on a daily basis. We conclude by discussing potential approaches for website owners and hosting/CDN providers for maximizing the privacy benefits of ESNI.Comment: In Proceedings of the 15th ACM Asia Conference on Computer and Communications Security (ASIA CCS '20), October 5-9, 2020, Taipei, Taiwa