Anti-cancer activities of curcumin and propolis extracts on MCF-7 breast cancer cell line model

It is evident that chemotherapy, which is one of the most preferred methods in cancer treatment, have several disadvantages and decrease the success rate of treatment. Therefore, identification and development of natural anti-cancer agents with less toxicity and side effects has recently become one of the areas of interest. In the present study, we reveal the potential anticancer activities of propolis and curcumin extracts and cisplatin on the breast cancer cell line (MCF-7). Individual and combinatorial treatments of propolis and curcumin was performed. MTT cell viability assay was used to determine the anti-proliferative activities of extracts, and Annexin V/PI double staining flow cytometric method was used to determine induction of apoptosis in breast cancer cells. In our study,the most significant reduction in MCF-7 cell viability was found to be 100 ?g/ml for cisplatin, 5 ?g/ml for curcumin and 160 ?g/ml for propolis. Apoptotic cell ratios were also found to be consistent with MTT findings. The highest proportion of apoptotic cells in the combinatorial study was found to be in the presence of cisplatin + propolis. In conclusion, here we show that combinatorial cisplatin + propolis treatments have significant anti-cancer activities on MCF-7 breast cancer cells in vitro. Studies involving natural products might be a new hope for complementary and alternative medicine by paving the way for clinical studies

Inula viscosa metanol ve hekzan ekstraktlarının antibakteriyel ve antikanserojenik etkilerinin incelenmesi

Objective: Inula viscosa is a perennial medicinal plant belonging to the Asteraceae family. This study aimed at investigating the antibacterial and anticarcinogenic activities of methanol and hexane extracts derived from I. viscosa. Methods: The antibacterial activity of different concentrations of I. viscosa methanol (1.56-800 mg/ml) and hexane (0.19-100 mg/ml) extracts was tested using the disc diffusion method. Listeria monocytogenes, Micrococcus luteus, Escherichia coli and Klebsiella pneumoniae were used as standard reference strains. Minimum inhibitory concentration (MIC) values of the extracts were made for each bacteria using the microdilution method. MIC value was determined as the lowest extract concentration at which growth was not observed. Minimum bactericide concentrations (MBC) were determined for all wells after MIC. The anticarcinogenic activity of I. viscosa was examined using the MTT test. MDA-MB-231 and HT-29 cells were stained with acridine orange and propidium iodide at the IC50 values of the extracts to determine the viable, apoptotic and necrotic cells. Results: Zone diameters of the methanol extract for L. monocytogenes, M. luteus, E. coli and K. pneumoniae were 8, 16, 7 and 9 mm, respectively. Zone diameters of the hexane extract for M. luteus, E. coli and K. pneumoniae were 14, 7 and 9 mm, respectively. MIC values of the methanol extract were 25 mg/ml for M. luteus, 200 mg/ml for L. monocytogenes and K. pneumoniae, and 400 mg/ml for E. coli. MIC values of the hexane extract were 400 mg/ml for L. monocytogenes, E. coli ve K. pneumoniae, and 100 mg/ml for M. luteus. MBC values were similar to MIC values. IC50 values for MDA-MB-231 and HT-29 cells were 25 mg/ml and 200 mg/ ml for the methanol extract, and 6.25 mg/ml and 12.5 mg/ml for the hexane extract, respectively. Depending on increased concentration levels, the presence of necrotic and early apoptotic cells was observed among MDA-MB-231 and HT-29 cells. Conclusion: Methanol and hexane extracts of I. viscosa were found to have antibacterial effects on the studied bacteria and anticarcinogenic activity on MDA-MB-231 and HT-29 cells. Conducting further studies to demonstrate the effects of these extracts on different cancer cell lines and bacterial strains will contribute to the field of complementary medicine. © 2022. All Rights Reserved

AN INVESTIGATION OF PRE-SERVICE TEACHERS’ ATTITUDES TOWARDS TEACHING PROFESSION IN REGARD TO SOME VARIABLES: THE CASE OF MIDDLE EAST TECHNICAL UNIVERSITY

The purpose of this study was to investigate the pre-service teachers' attitudes towards teaching profession and explore the effects of the certain variables (gender, GPA, age, department, motives for selecting teaching profession, and the level of mother and father education) on their attitudes. The participants of the study consisted of 344 undergraduate students, (282 females and 62 males) from five different departments at the faculty of education at Middle East Technical University during the fall semester of the 2015-2016 educational year. As a means of data collection, the "Attitude Measurement Scale for Teaching Profession" developed by Üstüner (2006) was applied. The scale was a one-dimensional 5-Likert type ranging from “strongly disagree (1)” to “strongly agree (5)” with 34 items. For the analysis of the data, Independent-Samples t-test and One-Way-ANOVA were used. According to the findings obtained from the study, while the attitudes of pre-service teachers towards the teaching profession showed a significant difference regarding gender, GPA, department, and motives for selecting the teaching profession, it did not show any significant difference for age and the level of mother and father education

Matematik Dersinde Procept (Nesne/Süreç) Teorisi Üzerine Yarı Deneysel Bir Çalışma

DergiPark: 750458tredProcept kavramı, aynı sembolün temsil ettiği, süreç/işlem ve kavramdan oluşan zihinsel bir nesne olarak tanımlanmıştır. (Gray ve Tall, 1991). Bu çerçevede yapılan çalışmanın amacı, mutlak değer konusunun öğretiminde analoji kullanımının, 6. Sınıf öğrencilerinin kavram ve işlem öğrenmelerinin procept düzeyinde gerçekleşmesi üzerindeki etkisinin incelenmesidir. Yarı deneysel çalışma ile gerçekleştirilen araştırmada geleneksel matematik öğretimi ve analoji destekli matematik öğretimi uygulanmıştır. Deney ve kontrol gruplu gerçekleştirilen araştırmanın çalışma grubunu, İzmir’deki bir ortaokulun 6. sınıf düzeyinde öğrenim gören 104 öğrencisi oluşturmaktadır. Deney grubunda mutlak değer konusuna yönelik kapsam ve görünüş geçerliliği sağlanmış analojilerle öğretim yapılırken, kontrol grubunda geleneksel matematik öğretimi gerçekleştirilmiş, uygulamalar sonucunda öğrencilere açık uçlu kavram ve işlem açıklama formu uygulanmıştır. Elde edilen veriler, açık uçlu yanıtların kategorilere ayrılıp belirli kodlarla temsil edilmesiyle ve bu kodların tekrar edilme sıklığına göre frekans (f) ve yüzde (%) değerleriyle incelenmiştir. Araştırmanın sonucunda, mutlak değer konusunun öğretiminde deney grubuna uygulanan analoji destekli matematik öğretiminin kontrol grubuna uygulanan geleneksel yönteme göre öğrencilerin kavramsal öğrenme düzeylerini artırmada daha etkili olduğu görülmüş, işlemsel bilgi düzeylerinde ise anlamlı bir fark oluşturmamıştır. Bu sonuç,mutlak değer konusunun öğretiminde deney grubuna uygulanan analoji destekli matematik öğretiminin kontrol grubuna uygulanan geleneksel yönteme göre öğrencilerin kavramsal öğrenme düzeylerini artırmada daha etkili olduğunu göstermektedir

The cycle outcomes of gnrh agonist triggering with different leuprolide acetate doses in high responder patients

70th Annual Meeting of the American-Society-for-Reproductive-Medicine -- OCT 18-22, 2014 -- Honolulu, HIAmerican Society for Reproductive Medicin

Re-evaluation of the symptoms of Hirayama disease through anatomical perspective

Hirayama disease is a rare disease of the anterior horn motor neuron caused by compression of the cervical spinal cord when the neck is flexed. Cervical myelopathy may accompany the disease. It is characterized by symmetrical or asymmetrical muscle weakness and atrophy of muscles innervated by lower cervical and upper thoracic motor neurons. We recorded two male cases of Hirayama disease between the ages of 15 and 21 based on magnetic resonance imaging (MRI) features obtained from the cervical neutral state and from the flexion position which appeared in the right upper extremity. Loss of strength and atrophy in the right upper extremities was existent in clinical findings of these patients. When MRI was taken in the flexion position, there were dilated veins as hypointense signal void on T2 weighted series in posterior epidural area. The contrast enhancement was seen on these veins. It was observed that the posterior dura was displaced anteriorly and the anterior subarachnoid space was narrow. In cases which show clinical findings such as atrophy and loss of strength, having normal MRI results obtained in the neutral position makes it difficult to diagnose Hirayama Disease. In case of a suspicion of Hirayama disease the diagnosis can be made more easily by MRI taken in the flexion position. These case reports aim to bring Hirayama disease to mind and optimize the management of affected individuals

NLO’nun beyin apselerinin tanı ve farmakolojik tedavi yönetimine etkileri

Aim: The present study aims to examine the effectiveness of the neutrophil-tolymphocyte ratio in the treatment and diagnosis of patients with brain abscesses. Methods: In this retrospective study, radiological, neurological, and surgical data obtained from the medical records of healthy volunteers (n = 10) who presented to the hospital for check-ups and patients with brain abscesses who were treated (n = 10) were evaluated statistically. Alpha significance value was accepted as 0.05). The preoperative neutrophil-to-lymphocyte ratio in cases diagnosed with brain abscesses showed statistical significance compared to that in the healthy volunteers (P 0,05). Sağlıklı bireylere oranla beyin apsesi tanısı alan olgulara ait preoperatif NLO değerlerinin istatistiksel olarak anlamlılık (P<0,05) gösterdiği ve NLO değerinin sağlıklı bireylere oranla yaklaşık 3,31 kat artış gösterdiği kaydedildi. Sonuç: NLO oranı ile abse boyutu arasında (r=0,662; P=0,037) pozitif yönde kuvvetli ilişki bulunmaktadır. Artış gösteren NLO, beyin apsesinin tanısında erken uyarı sinyali olarak hizmet edebilir

Mechanical Properties of Acorn and Pine Cone Filled Polymer Composites

In this work, the composites consisted of epoxy resin reinforced with acorn and pine cone fillers/powders were investigated. Both hardness and tensile properties of composites were evaluated dependent on filler type and content. The waste fillers were added into the polymer with three different ratios (10%, 20% and 30% weight percent) and manufactured with casting method. Based on the quasi-static tensile tests and hardness measurements, the pine cone based composites showed slightly better results compared to the acorn reinforced samples. In addition to that the composites independent of filler type showed higher tensile and hardness properties with increasing the particle content

Does Maxillary Protraction with Alt-RAMEC Protocol Affect Inferior Sclera Exposure? A Controlled 3dMD Study

Objective: The purpose of this controlled retrospective study was to measure and evaluate the inferior sclera exposure changes using 3dMD stereophotogrammetric images in a prepubertal Class III patient sample that underwent maxillary protraction with alternate rapid maxillary expansions and constrictions protocol followed by facemask.Methods: The study group included 15 prepubertal patients (mean age: 9.85 ± 1.44 years) with Class III malocclusion due to maxillary retrognathism. Nine weeks of alternate rapid maxillary expansions and constrictions protocol was followed prior to 7 months of face mask treatment and 3 months of retention with Bionator. Pretreatment (T0) and post-retention (T1) lateral cephalometric radiographs and 3dMD images were retrieved from clinical archive. The same records were used for a control group of 15 well-matched, untreated patients (mean age: 9.4 ± 0.79 years). The distance between the upper eyelid margin and the lower eyelid margin was recorded as the overall eye height (E), and the distance between inferior limbus and the lower eyelid margin was recorded as inferior sclera exposure (S). The S : E ratio in percentage was calculated. Sella-nasion-A point angle (SNA) was used as the skeletal variable.Results: SNA angle, right S : E, and left S : E changed significantly in both groups at T1-T0. The intergroup comparison was highly significant for SNA angle but was not significant for right and left S : E variables.Conclusion: The S : E ratio decreased significantly in both alternate rapid maxillary expansions and constrictions/facemask and the control groups. However, the change in S : E ratio between groups was not significant