Comparative genomic analysis of two Chilean Renibacterium salmoninarum isolates and the type strain ATCC 33209T

Indexación: Scopus.Two previously characterized Chilean isolates (H-2 and DJ2R), obtained from cage-cultured Atlantic salmon with clinical signs of bacterial kidney disease in southern Chile, were used (Bethke et al. 2016, 2017). The bacteria were routinely cultured in KDM-2 agar for 15–20 days at 15°C. For sequencing, genomic DNA of the two isolates was extracted using the InstaGene Purification Matrix (Bio-Rad) according to manufacturer instructions. The DJ2R genome was sequenced using an Illumina MiSeq platform with 2 ⨯ 250 paired-end reads by the AUSTRAL-omics Institute, hosted by the Universidad Austral de Chile (Valdivia, Chile). Using the same technology and parameters, H-2 genomic DNA was sequenced by the Central Support Service for Experimental Research (SCSIE, Spanish acronym) at the University of Valencia (Valencia, Spain).This work was supported by funding of the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT, Chile) [Grant Numbers FONDAP No. 15110027 and FONDECYT No. 1150695]. J.B. also acknowledges support received by CONICYT [Doctoral Scholarship No. 21140421].Renibacterium salmoninarum, a slow-growing facultative intracellular pathogen belonging to the high C+G content Actinobacteria phylum, is the causative agent of bacterial kidney disease, a progressive granulomatous infection affecting salmonids worldwide. This Gram-positive bacterium has existed in the Chilean salmonid industry for >30 years, but little or no information is available regarding the virulence mechanisms and genomic characteristics of Chilean isolates. In this study, the genomes of two Chilean isolates (H-2 and DJ2R)were sequenced, and a search was conducted for genes and proteins involved in virulence and pathogenicity, andwecompare with the type strain ATCC 33209T genome. The genome sizes of H-2 and DJ2R are 3,155,332 bp and 3,155,228 bp, respectively. They genomes presented six ribosomal RNA, 46 transcription RNA, and 25 noncodingRNA, and both had the same 56.27% G+C content described for the type strain ATCC 33209 T. A total of 3,522 and 3,527 coding sequences were found for H-2 and DJ2R, respectively. Meanwhile, the ATCC 33209T type strain had 3,519 coding sequences. The in silico genome analysis revealed a genes related to tricarboxylic acid cycle, glycolysis, iron transport and others metabolic pathway. Also, the data indicated that R salmoninarum may have a variety of possible virulence-factor and antibiotic-resistance strategies. Interestingly, many of genes had high identities with Mycobacterium species, a known pathogenic Actin obacteria bacterium. In summary, this study provides the first insights into and initial steps towards understanding the molecular basis of antibiotic resistance, virulence mechanisms and host/environment adaptation in twoChilean R. salmoninarum isolates that contain proteins of which were similar to those of Mycobacterium. Furthermore, important information is presented that could facilitate the development of preventive and treatment measures against R. salmoninarum in Chile and worldwide. © The Author(s) 2018.https://academic.oup.com/gbe/article/10/7/1816/504777

Information theory of quantum systems with some hydrogenic applications

The information-theoretic representation of quantum systems, which complements the familiar energy description of the density-functional and wave-function-based theories, is here discussed. According to it, the internal disorder of the quantum-mechanical non-relativistic systems can be quantified by various single (Fisher information, Shannon entropy) and composite (e.g. Cramer-Rao, LMC shape and Fisher-Shannon complexity) functionals of the Schr\"odinger probability density. First, we examine these concepts and its application to quantum systems with central potentials. Then, we calculate these measures for hydrogenic systems, emphasizing their predictive power for various physical phenomena. Finally, some recent open problems are pointed out.Comment: 9 pages, 3 figure

Nivel de conocimiento sobre higiene bucodental y protésica en cuidadores de ELEAM en la región de Valparaíso, Chile 2016

Tesis (Cirujano Dentista)... la información obtenida en el presente estudio, que tiene como propósito valorar el nivel de conocimiento sobre higiene oral en cuidadores de adultos mayores residentes en ELEAM de la región de Valparaíso, Chile 2016, permitirá orientar estrategias para que los cuidadores se actualicen y adquieran nuevos conocimientos o habilidades, y se motiven para desarrollar intervenciones preventivas orientadas a mejorar la salud bucal y la salud general de esta población

Shape index, Brouwer degree and Poincaré-Hopf theorem

In this paper we study the relationship of the Brouwer degree of a vector field with the dynamics of the induced flow. Analogous relations are studied for the index of a vector field. We obtain new forms of the Poincaré-Hopf theorem and of the Borsuk and Hirsch antipodal theorems. As an application, we calculate the Brouwer degree of the vector field of the Lorenz equations in isolating blocks of the Lorenz strange set.Depto. de Álgebra, Geometría y TopologíaFac. de Ciencias MatemáticasFALSEMinisterio de Ciencia, Innovación y Universidadesunpu

Identification and measurement of fibers in scanning electron microscopy images using a high-order correlation process

Includes bibliographical references.This work describes the development of a dedicated system capable of identifying, measuring and counting various types of fibers and other objects in digitized scanning electron micrograph (SEM) imagery. The system uses a recursive high order correlation (HOC) process to extract the corner pixels of the fibers. The objects are defined by grouping connected corners, so that morphometric analysis can be performed. The method developed performs satisfactorily when the density of fibers per image ranges from low to medium. Simulation results for several cases are presented, along with a discussion on the capabilities and limitations of the current version of the system.This work was supported by Schuller, Inc., Denver, CO

Asymptotic Smoothness, Convex Envelopes and Polynomial Norms

We introduce a suitable notion of asymptotic smoothness on infinite dimensional Banach spaces, and we prove that, under some structural restrictions on the space, the convex envelope of an asymptotically smooth function is asymptotically smooth. Furthermore, we study convexity and smoothness properties of polynomial norms, and we obtain that a polynomial norm of degree N has modulus of convexity of power type N

Integral valorization of Acacia dealbata wood in organic medium catalyzed by an acidic ionic liquid

In this work, a novel delignification process was proposed for the fractionation of invasive species such as Acacia dealbata wood. Organosolv process catalyzed with an acidic ionic liquid, 1-butyl-3-methylimidazolium hydrosulfate was evaluated to obtain cellulose-enriched solids and liquid fractions rich in hemicelluloses derived compounds and lignin. Under selected operating conditions (190 °C, 60% ethanol, 60 min of reaction time and 0.6 g 1-butyl-3-methylimidazolium hydrosulfate/g wood), high solubilization of lignin and hemicelluloses and cellulose recovery (87.5%, 88.7% and 88.3%, respectively), with a pulp yield of 43.1% were achieved. Moreover, 62.6 % of lignin was recovered by precipitation from the black liquor (composed mainly by 4.43 g xylose/L, 7.66 g furfural/L and 3.59 g acetic acid/L). In addition, enzymatic digestibility of delignified wood was also assayed. Overall, this work presents an alternative biorefinery scheme based in the use of environmentally friendly solvent and catalyst for selective fractionation of A. dealbata wood.The authors acknowledge the financial support received from the Spanish “Ministry of Economy and Competitiveness” (Project CTQ2017- 82962-R) and from “Xunta de Galicia” (GRC ED431C 2018/47 and Centro Singular de Investigacion ´ Biom´edica “CINBIO”). These projects are partially funded by the FEDER Program of the European Union (“Unha maneira de facer Europa”). A. Romaní thanks BioTecNorte operation (NORTE-01–0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersio