miRNA-223 expression in patient-derived eutopic and ectopic endometrial stromal cells and its effect on epithelial-to-mesenchymal transition in endometriosis

Objective: This study was designed to evaluate the expression of microRNA-223 (miRNA-223) in patient-derived eutopic and ectopic endometrial stromal cells (SCs). Given the fact that miRNA-223 was previously shown to be upregulated in these cells and that this upregulation has been linked to epithelial-to-mesenchymal transition (EMT) during endometriosis, this study aimed to further explore the expression of miRNA-223, its effect in endometriosis, and the mechanisms underlying its effects. Methods: Endometrial tissue was collected from 26 patients with endometriosis and 14 patients with hysteromyoma (control group). Primary endometrial SCs were isolated and cultured from several endometrial samples and miRNA-223 expression was evaluated using qRT-PCR. Cells were then transfected with a miRNA-223 overexpression lentiviral vector (sh-miR-223 cells) or an empty control (sh-NC cells) and then used to monitor the effects of miRNA-223 on the expression of several EMT-associated proteins, including N-cadherin, vimentin, and Slug, using western blot. Cellular migration, invasion, and proliferation were then evaluated using a wound healing, Transwell, and CCK-8 assay, respectively. Flow cytometry was used to detect apoptosis. Results: There was a significant decrease in the expression of miRNA-223 in both eutopic and ectopic endometrial SCs (p < 0.05) whereas upregulation of miRNA-223 inhibited the expression of EMT-related molecules and reduced cell migration, invasion, and proliferation. High levels of miRNA-223 also promoted apoptosis. Conclusion: miRNA-223 expression decreased in endometrial SCs from endometriosis patients, which may facilitate the differential regulation of EMT during endometriosis. Clinical Trial registration number: SWYX2020-211

Oligopoly in grain production and consumption: an empirical study on soybean international trade in China

What has been neglected in much of the existing studies of the influence of seasonal and regional characteristics of agriculture on the market power and national security. This paper constructs a multivariate equations model to investigate the monopoly power of seasonal suppliers and national security in China’s soybean market. The results show no relationship between market share and monopoly power; and that CR3 and HHI show China’s soybean import market has been the highest oligopoly type, but the model suggest that exporters (the U.S., Brazil and Argentina) have very weak monopoly power and China has no monopsony power; and that the performance of some exporters’ soybeans is affected by others, while others are relatively independent in market. This is due to the non-substitutability of the product, the non-substitutability of the buyer and the seller, etc., which causes the mutual dependence of the seller and buyer, and their market power cancel each other out. The seasonality and regionality of soybean production is the root. Considering national security, it is necessary to take the seasonal and regional characteristics of exporters into account to disperse trade risks and oppose monopolisation of international food production and trad

The Effect of Artificial Intelligence (AI) on Firm Labor Structure

This paper aims to study the effect of AI on firm labor structure. Using a unique panel data of over 1300 publicly listed companies in China from 2007 to 2018, we study the effect of AI on firms’ labor composition measured by labor force’s education levels. We further compare the effect of AI on firms in the manufacturing sector to the effect on firms in the service sector. Our analysis generates two major findings. First, the use of AI leads to a larger labor demand increase for jobs requiring lower education levels than those requiring higher education levels. Second, the effect is stronger in the service sector than in the manufacturing sector. These findings contradict predictions of the “skill-biased technological change” (SJTB) and U-shaped “job polarization” effects proposed in the prior literature. We propose that “technology-enabled deskilling” effect is driving the effect of AI on labor structure

Evaluation of sustainable development of the Bohai Sea Rim based on integrated land–sea management: a multi-system coupling and coordination study at coastal, provincial, and city level

Based on the Integrated Land–Sea Management, this study established a theoretical framework for the sustainability of coastal regions by combining sustainable development with coupling coordination theory. The improved coupling coordination model was used to analyze the sustainable development of the Bohai Rim and its coastal provinces and cities from 2006 to 2020. Our implications were as follows: (1) The theoretical framework showed an S-shaped spiral trend, and the empirical results on the Bohai Rim were consistent with the trajectory conclusions. (2) The economic subsystem played a crucial role in the system’s evolution toward sustainable development. (3) The region and city models demonstrated consistent coupling and coordination development degrees. However, the consistency was not completely synchronous. Conscious eco-environmental governance activities can promote benign interactions among systems and improve this relationship. (4) The sustainable development of coastal cities is different from that of the provinces in which they are located. It merely demonstrates their relative status among all coastal cities and does not fully represent the wider region in which they are located. The findings suggest that adaptive policies, whether economic, social or environmental, can promote sustainable development. Economic stimulus policies can promote a transition of sustainable development; in the economic downturn, the adaptive environmental policy is realized by adjusting the relationship between subsystems to promote the coordination of regional systems, preparing for the next sustainable system transition. The established theoretical model and improvised mathematical method can be extended to study various coastal region

Slippery for scaling resistance in membrane distillation: a novel porous micropillared superhydrophobic surface

Scaling in membrane distillation (MD) is a key issue in desalination of concentrated saline water, where the interface property between the membrane and the feed become critical. In this paper, a slippery mechanism was explored as an innovative concept to understand the scaling behavior in membrane distillation for a soluble salt, NaCl. The investigation was based on a novel design of a superhydrophobic polyvinylidene fluoride (PVDF) membrane with micro-pillar arrays (MP-PVDF) using a micromolding phase separation (μPS) method. The membrane showed a contact angle of 166.0 ± 2.3° and the sliding angle of 15.8 ± 3.3°. After CF4 plasma treatment, the resultant membrane (CF4-MP-PVDF) showed a reduced sliding angle of 3.0o. In direct contact membrane distillation (DCMD), the CF4-MP-PVDF membrane illustrated excellent anti-scaling in concentrating saturated NaCl feed. Characterization of the used membranes showed that aggregation of NaCl crystals occurred on the control PVDF and MP-PVDF membranes, but not on the CF4-MP-PVDF membrane. To understand this phenomenon, a “slippery” theory was introduced and correlated the sliding angle to the slippery surface of CF4-MP-PVDF and its anti-scaling property. This work proposed a well-defined physical and theoretical platform for investigating scaling problems in membrane distillation and beyond

A Novel Signal Transduction Pathway that Modulates <i>rhl</i> Quorum Sensing and Bacterial Virulence in <i>Pseudomonas aeruginosa</i>

The rhl quorum-sensing (QS) system plays critical roles in the pathogenesis of P. aeruginosa. However, the regulatory effects that occur directly upstream of the rhl QS system are poorly understood. Here, we show that deletion of gene encoding for the two-component sensor BfmS leads to the activation of its cognate response regulator BfmR, which in turn directly binds to the promoter and decreases the expression of the rhlR gene that encodes the QS regulator RhlR, causing the inhibition of the rhl QS system. In the absence of bfmS, the Acka-Pta pathway can modulate the regulatory activity of BfmR. In addition, BfmS tunes the expression of 202 genes that comprise 3.6% of the P. aeruginosa genome. We further demonstrate that deletion of bfmS causes substantially reduced virulence in lettuce leaf, reduced cytotoxicity, enhanced invasion, and reduced bacterial survival during acute mouse lung infection. Intriguingly, specific missense mutations, which occur naturally in the bfmS gene in P. aeruginosa cystic fibrosis (CF) isolates such as DK2 strains and RP73 strain, can produce BfmS variants (BfmSL181P, BfmSL181P/E376Q, and BfmSR393H) that no longer repress, but instead activate BfmR. As a result, BfmS variants, but not the wild-type BfmS, inhibit the rhl QS system. This study thus uncovers a previously unexplored signal transduction pathway, BfmS/BfmR/RhlR, for the regulation of rhl QS in P. aeruginosa. We propose that BfmRS TCS may have an important role in the regulation and evolution of P. aeruginosa virulence during chronic infection in CF lungs.</p

Effects of lower troposphere vertical mixing on simulated clouds and precipitation over the Amazon during the wet season

Planetary boundary layer (PBL) schemes parameterize unresolved turbulent mixing within the PBL and free troposphere (FT). Previous studies reported that precipitation simulation over the Amazon in South America is quite sensitive to PBL schemes and the exact relationship between the turbulent mixing and precipitation processes is, however, not disentangled. In this study, regional climate simulations over the Amazon in January-February 2019 are examined at process level to understand the precipitation sensitivity to PBL scheme. The focus is on two PBL schemes, the Yonsei University (YSU) scheme, and the asymmetric convective model v2 (ACM2) scheme, which show the largest difference in the simulated precipitation. During daytime, while the FT clouds simulated by YSU dissipate, clouds simulated by ACM2 maintain because of enhanced moisture supply due to the enhanced vertical moisture relay transport process: 1) vertical mixing within PBL transports surface moisture to the PBL top, and 2) FT mixing feeds the moisture into the FT cloud deck. Due to the thick cloud deck over Amazon simulated by ACM2, surface radiative heating is reduced and consequently the convective available potential energy (CAPE) is reduced. As a result, precipitation is weaker from ACM2. Two key parameters dictating the vertical mixing are identified, p, an exponent determining boundary layer mixing and λ, a scale dictating FT mixing. Sensitivity simulations with altered p, λ, and other treatments within YSU and ACM2 confirm the precipitation sensitivity. The FT mixing in the presence of clouds appears most critical to explain the sensitivity between YSU and ACM2.This work was primarily supported by grant no. 20163646499 from the Universidad Nacional de San Agustín de Arequipa (UNSA) of Peru through the IREES/LASI Global Change and Human Health Institute. The first author is partially supported by the National Mesonet Program grant #10558200 and DOE ASR project (DE-SC0021159). Ming Xue was also supported by NSF grants AGS-1917701.YesAn edited version of this paper was published by AGU. Published 2023, American Geophysical Union. Preprint available at https://doi.org/10.22541/essoar.167458066.61800879/v

Anoctamin 4 Channel Currents Activate Glucose-Inhibited Neurons in the Mouse Ventromedial Hypothalamus During Hypoglycemia

Glucose is the basic fuel essential for maintenance of viability and functionality of all cells. However, some neurons - namely, glucose-inhibited (GI) neurons - paradoxically increase their firing activity in low-glucose conditions and decrease that activity in high-glucose conditions. The ionic mechanisms mediating electric responses of GI neurons to glucose fluctuations remain unclear. Here, we showed that currents mediated by the anoctamin 4 (Ano4) channel are only detected in GI neurons in the ventromedial hypothalamic nucleus (VMH) and are functionally required for their activation in response to low glucose. Genetic disruption of the Ano4 gene in VMH neurons reduced blood glucose and impaired counterregulatory responses during hypoglycemia in mice. Activation of VMHAno4 neurons increased food intake and blood glucose, while chronic inhibition of VMHAno4 neurons ameliorated hyperglycemia in a type 1 diabetic mouse model. Finally, we showed that VMHAno4 neurons represent a unique orexigenic VMH population and transmit a positive valence, while stimulation of neurons that do not express Ano4 in the VMH (VMHnon-Ano4) suppress feeding and transmit a negative valence. Together, our results indicate that the Ano4 channel and VMHAno4 neurons are potential therapeutic targets for human diseases with abnormal feeding behavior or glucose imbalance

EGFR and HER2 expression in primary cervical cancers and corresponding lymph node metastases: Implications for targeted radiotherapy

<p>Abstract</p> <p>Background</p> <p>Proteins overexpressed on the surface of tumor cells can be selectively targeted. Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) are among the most often targeted proteins. The level and stability of expression in both primary tumors and corresponding metastases is crucial in the assessment of a receptor as target for imaging in nuclear medicine and for various forms of therapy. So far, the expression of EGFR and HER2 has only been determined in primary cervical cancers, and we have not found published data regarding the receptor status in corresponding metastatic lesions. The goal of this study was to evaluate whether any of these receptors are suitable as target for clinical diagnosis and therapy.</p> <p>Methods</p> <p>Expression of EGFR and HER2 was investigated immunohistochemically in both lymph node metastases and corresponding primary cervical cancers (n = 53). HER2 and EGFR expression was scored using HercepTest criteria (0, 1+, 2+ or 3+).</p> <p>Results</p> <p>EGFR overexpression (2+ or 3+) was found in 64% (35/53) of the primary cervical tumors and 60% (32/53) of the corresponding lymph node metastases. There was a good concordance between the primary tumors and the paired metastases regarding EGFR expression. Only four patients who had 2+ or 3+ in the primary tumors changed to 0 or 1+ in lymph node metastases, and another two cases changed the other way around. None of the primary tumors or the lymph node metastases expressed HER2 protein.</p> <p>Conclusion</p> <p>The EGFR expression seems to be common and stable during cervical cancer metastasis, which is encouraging for testing of EGFR targeted radiotherapy. HER2 appears to be of poor interest as a potential target in the treatment of cervical cancer.</p