miR-218 targets survivin and regulates resistance to chemotherapeutics in breast cancer

Multidrug resistance (MDR) remains one of the most significant obstacles in breast cancer treatment, and this process often involves dysregulation of a great number of microRNAs (miRNAs). Some miRNAs are indicators of drug resistance and confer resistance to chemotherapeutic drugs, although our understanding of this complex process is still incomplete. We have used a combination of miRNA profiling and real-time PCR in two drug-resistant derivatives of MCF-7 and Cal51 cells. Experimental modulation of miR expression has been obtained by retroviral transfection. Taxol and doxorubicin IC50 values were obtained by short-term drug sensitivity assays. Apoptosis was determined by flow cytometry after annexin V staining, by caspase 3/7 and caspase 9 activity assays and the levels of apoptosis-related proteins bcl-2 and bax by real-time PCR and Western blot. miR target was studied using transient transfection of luciferase constructs with the 3 untranslated regions (UTR) of target mRNAs. Small interfering RNA-mediated genetic knock-down was performed in MDR cells and its modulatory effect on apoptosis examined. The effect of miRNA on tumorigenicity and tumor drug response was studied in mouse xenografts. miRNA profiling of two drug-resistant breast cancer cell models indicated that miR-218 was down-regulated in both MCF-7/A02 and CALDOX cells. Ectopic expression of miR-218 resensitized both drug-resistant cell lines to doxorubicin and taxol due to an increase in apoptosis. miR-218 binds survivin (BIRC5) mRNA 3-UTR and down-regulated reporter luciferase activity. Experimental down-regulation of survivin by RNA interference in drug-resistant cells did mimic the sensitization observed when miRNA-218 was up-regulated. In addition, resensitization to taxol was also observed in mouse tumor xenografts from cells over-expressing miR-218. miR-218 is involved in the development of MDR in breast cancer cells via targeting survivin and leading to evasion of apoptosis. Targeting miR-218 and survivin may thus provide a potential strategy for reversing drug resistance in breast cancer

Analysis of excited quark propagator effects on neutron charge form factor

The charge form factor and charge radius of neutron are investigated in the perturbative chiral quark model (PCQM) with considering both the ground and excited states in the quark propagator. A Cornell-like potential is extracted in accordance with the predetermined ground state quark wavefunction, and the excited quark states are derived by solving the Dirac equation with the extracted PCQM potential numerically. The study reveals that the contributions of the excited quark states are considerably influential in the charge form factor and charge radius of neutron as expected, and the total results are significantly improved and increased by nearly four times by including the excited states in the quark propagator. The theoretical PCQM results are found, including the ground and excited quark propagators, in good agreement with the recent lattice QCD values at pion mass of about 130 MeV.Comment: 8 pages, 8 figure

Opportunistic Relaying in Time Division Broadcast Protocol with Incremental Relaying

In this paper, we investigate the performance of time division broadcast protocol (TDBC) with incremental relaying (IR) when there are multiple available relays. Opportunistic relaying (OR), i.e., the “best” relay is select for transmission to minimize the system’s outage probability, is proposed. Two OR schemes are presented. The first scheme, termed TDBC-OIR-I, selects the “best” relay from the set of relays that can decode both flows of signal from the two sources successfully. The second one, termed TDBC-OIR-II, selects two “best” relays from two respective sets of relays that can decode successfully each flow of signal. The performance, in terms of outage probability, expected rate (ER), and diversity-multiplexing tradeoff (DMT), of the two schemes are analyzed and compared with two TDBC schemes that have no IR but OR (termed TDBC-OR-I and TDBC-OR-II accordingly) and two other benchmark OR schemes that have no direct link transmission between the two sources

An efficient hybrid model and dynamic performance analysis for multihop wireless networks

Multihop wireless networks can be subjected to nonstationary phenomena due to a dynamic network topology and time varying traffic. However, the simulation techniques used to study multihop wireless networks focus on the steady-state performance even though transient or nonstationary periods will often occur. Moreover, the majority of the simulators suffer from poor scalability. In this paper, we develop an efficient performance modeling technique for analyzing the time varying queueing behavior of multihop wireless networks. The one-hop packet transmission (service) time is assumed to be deterministic, which could be achieved by contention-free transmission, or approximated in sparse or lightly loaded multihop wireless networks. Our model is a hybrid of time varying adjacency matrix and fluid flow based differential equations, which represent dynamic topology changes and nonstationary network queues, respectively. Numerical experiments show that the hybrid fluid based model can provide reasonably accurate results much more efficiently than standard simulators. Also an example application of the modeling technique is given showing the nonstationary network performance as a function of node mobility, traffic load and wireless link quality. © 2013 IEEE

The polymeric conformational effect on capacitive deionization performance of graphene oxide/polypyrrole composite electrode

Exploitation of novel faradic materials is an alternative implementation for solving the problem of poor specific electrosorption capacity that conventional carbon materials are encountered in capacitive deionization. Particularly, composite electrode is just a suitable choice because of its potentially high ion-storage ability. Herein, a cyclic voltammetric treatment method with different low limit of potential window was used to manipulate the polymeric conformation and doping level of graphene oxide/polypyrrole (GO/PPy) composite electrode. Based on it, the effect of polymeric structure on the electrosorption performance was systematically studied. When the low limit of potential window is shifted negatively enough, the irreversible polymeric conformational shrinks of GO/PPy are promoted, which not only hinders the insertion process of ions, but also decreases the doping level of polymer due to the intensive interchain-action produced by more entangled polymeric chain. Thus, the number of intercalated ions should decrease, which is expressed by electrochemical impedance spectroscopy (EIS) results and is proportional to the electrosorption capacity of GO/PPy composite electrode in membrane capacitive deionization (MCDI) process. Our work suggests that the less packing density, higher doping level and more charge delocalization on PPy backbone in electrode are beneficial to enhance its capacitive deionization performance