Validation of 7 Years in-Flight HY-2A Calibration Microwave Radiometer Products Using Numerical Weather Model and Radiosondes

Haiyang-2A (HY-2A) has been working in-flight for over seven years, and the accuracy of HY-2A calibration microwave radiometer (CMR) data is extremely important for the wet troposphere delay correction (WTC) in sea surface height (SSH) determination. We present a comprehensive evaluation of the HY-2A CMR observation using the numerical weather model (NWM) for all the data available period from October 2011 to February 2018, including the WTC and the precipitable water vapor (PWV). The ERA(ECMWF Re-Analysis)-Interim products from European Centre for Medium-Range Weather Forecasts (ECMWF) are used for the validation of HY-2A WTC and PWV products. In general, a global agreement of root-mean-square (RMS) of 2.3 cm in WTC and 3.6 mm in PWV are demonstrated between HY-2A observation and ERA-Interim products. Systematic biases are revealed where before 2014 there was a positive WTC/PWV bias and after that, a negative one. Spatially, HY-2A CMR products show a larger bias in polar regions compared with mid-latitude regions and tropical regions and agree better in the Antarctic than in the Arctic with NWM. Moreover, HY-2A CMR products have larger biases in the coastal area, which are all caused by the brightness temperature (TB) contamination from land or sea ice. Temporally, the WTC/PWV biases increase from October 2011 to March 2014 with a systematic bias over 1 cm in WTC and 2 mm in PWV, and the maximum RMS values of 4.62 cm in WTC and 7.61 mm in PWV occur in August 2013, which is because of the unsuitable retrieval coefficients and systematic TB measurements biases from 37 GHz band. After April 2014, the TB bias is corrected, HY-2A CMR products agree very well with NWM from April 2014 to May 2017 with the average RMS of 1.68 cm in WTC and 2.65 mm in PWV. However, since June 2017, TB measurements from the 18.7 GHz band become unstable, which led to the huge differences between HY-2A CMR products and the NWM with an average RMS of 2.62 cm in WTC and 4.33 mm in PWV. HY-2A CMR shows high accuracy when three bands work normally and further calibration for HY-2A CMR is in urgent need. Furtherly, 137 global coastal radiosonde stations were used to validate HY-2A CMR. The validation based on radiosonde data shows the same variation trend in time of HY-2A CMR compared to the results from ECMWF, which verifies the results from ECMWF

Super-heated Steam Drying: an Innovation of Clean Coal Technology for Lignite

A comprehensive overview of the status of lignite pre-drying technology is given in this study. The practical problems of high energy consumption, high investment and poor safety exist in the lignite drying using the traditional thermal drying, for the reason that the super-heated steam drying technology for lignite is put forward. The mechanism experiment research and pilot-scale test research of the super-heated steam drying technology for lignite were carried out, and the results show that the drying efficiency of the super-heated steam is better than the hot air at given conditions, the lignite drying can be accomplished stably and continuously and the drying product can meet the requirement of the industrial application of the lignite fired power plant. A more innovative approach as a new lignite Super-heated steam low-rank Coal Upgrading (SCU) is proposed by Energy Conservation Research Center of Shandong Academy of Sciences. The technical advancement in terms of energy-saving and safety among the applied technologies are compared and analyzed, and the results show this technology has the advantages of low energy consumption, high safety and energy saving. To sum up, the exploitation of the super-heated steam drying technology for lignite fired power plant can promote the development of the energy and power industry, and the technology has wide application prospect

Role of LECT2 in exacerbating atopic dermatitis: insight from in vivo and in vitro models via NF-κB signaling pathway

Leukocyte cell-derived chemotaxin 2 (LECT2) is linked to various immune diseases. Previously, we reported that serum LECT2 levels correlate with disease severity in atopic dermatitis (AD) patients. To investigate the role of LECT2 in AD and elucidate its potential mechanisms, we used LECT2 to treat an AD mouse model induced by 1-Chloro-2,4-dinitrobenzene (DNCB) in LECT2 knockout (KO) and wild-type (WT) mice, and an AD cell model using TNF-α/IFN-γ-induced HaCaT cells. Inflammatory factors and barrier proteins were analyzed by histology, immunohistochemistry, RT-qPCR, ELISA, and Western Blot. Activation of the NF-κB signaling pathway was evaluated by Western Blot and immunofluorescence. In the AD mouse model, LECT2 treatment increased epidermal and dermal thickness, mast cell infiltration, and downregulated barrier proteins. Inflammatory factors were increased in skin lesions and serum. In the AD cell model, LECT2 decreased barrier protein levels and increased inflammatory factor levels, enhancing NF-κB P65 nuclear translocation. These results indicate that LECT2 exacerbates AD-like responses by dysregulating the NF-κB signaling pathway, highlighting its potential as a therapeutic target for AD management

Validating HY-2A CMR precipitable water vapor using ground-based and shipborne GNSS observations

The calibration microwave radiometer (CMR) on board the Haiyang-2A (HY-2A) satellite provides wet tropospheric delay correction for altimetry data, which can also contribute to the understanding of climate system and weather processes. The ground-based global navigation satellite system (GNSS) provides precise precipitable water vapor (PWV) with high temporal resolution and could be used for calibration and monitoring of the CMR data, and shipborne GNSS provides accurate PWV over open oceans, which can be directly compared with uncontaminated CMR data. In this study, the HY-2A CMR water vapor product is validated using ground-based GNSS observations of 100 International GNSS Service (IGS) stations along the global coastline and 56 d shipborne GNSS observations over the Indian Ocean. The processing strategy for GNSS data and CMR data is discussed in detail. Special efforts were made in the quality control and reconstruction of contaminated CMR data. The validation result shows that HY-2A CMR PWV agrees well with ground-based GNSS PWV with 2.67 mm as the root mean square (rms) within 100 km. Geographically, the rms is 1.12 mm in the polar region and 2.78 mm elsewhere. The PWV agreement between HY-2A and shipborne GNSS shows a significant correlation with the distance between the ship and the satellite footprint, with an rms of 1.57 mm for the distance threshold of 100 km. Ground-based GNSS and shipborne GNSS agree with HY-2A CMR well

Characterization of Fungal nirK-Containing Communities and N2O Emission From Fungal Denitrification in Arable Soils

Fungal denitrifiers play important roles in soil nitrogen cycling, but we have very limited knowledge about their distribution and functions in ecosystems. In this study, three types of arable soils were collected across different climate zones in China, including quaternary red clay soils, alluvial soils, and black soils. The composition and abundance of fungal nirK-containing denitrifiers was determined by MiSeq high-throughput sequencing and qPCR, respectively. Furthermore, a substrate-induced inhibition approach was used to explore N2O emissions from fungal denitrification. The results showed that the arable soils contained a wide range of nirK-containing fungal denitrifiers, with four orders and eight genera. Additionally, approximately 57.30% of operational taxonomic unit (OTUs) belonged to unclassified nirK-containing fungi. Hypocreales was the most predominant order, with approximately 40.51% of the total number of OTUs, followed by Sordariales, Eurotiales, and Mucorales. It was further indicated that 53% of fungal nirK OTUs were shared by the three types of soils (common), and this group of fungi comprised about 98% of the total relative abundance of the nirK-containing population, indicating that the distribution of fungal nirK-containing denitrifiers was quite homogenous among the soil types. These common OTUs were determined by multiple soil characteristics, while the composition of unique OTUs was manipulated by the specific properties of each soil type. Furthermore, fungal N2O emissions were significantly and positively correlated with fungal nirK abundance in the soils, whereas it was not clearly related to fungal nirK compositions. In conclusion, although the arable soils hosted diverse nirK-containing fungal denitrifiers, fungal nirK compositions were highly homogenous among the soil types, which could be a consequence of enduring agricultural practices. The abundance of fungal nirK-containing denitrifiers, rather than their composition, may play more significant roles in relation to N2O emission from fungal denitrification

ESX Secretion-Associated Protein C From Mycobacterium tuberculosis Induces Macrophage Activation Through the Toll-Like Receptor-4/Mitogen-Activated Protein Kinase Signaling Pathway

Mycobacterium tuberculosis, as a facultative intracellular pathogen, can interact with host macrophages and modulate macrophage function to influence innate and adaptive immunity. Proteins secreted by the ESX-1 secretion system are involved in this relationship. Although the importance of ESX-1 in host-pathogen interactions and virulence is well-known, the primary role is ascribed to EsxA (EAST-6) in mycobacterial pathogenesis and the functions of individual components in the interactions between pathogens and macrophages are still unclear. Here, we investigated the effects of EspC on macrophage activation. The EspC protein is encoded by an espA/C/D cluster, which is not linked to the esx-1 locus, but is essential for the secretion of the major virulence factors of ESX-1, EsxA and EsxB. Our results showed that both EspC protein and EspC overexpression in M. smegmatis induced pro-inflammatory cytokines and enhanced surface marker expression. This mechanism was dependent on Toll-like receptor 4 (TLR4), as demonstrated using EspC-treated macrophages from TLR4−/− mice, leading to decreased pro-inflammatory cytokine secretion and surface marker expression compared with those from wild-type mice. Immunoprecipitation and immunofluorescence assays showed that EspC interacted with TLR4 directly. Moreover, EspC could activate macrophages and promote antigen presentation by inducing mitogen-activated protein kinase (MAPK) phosphorylation and nuclear factor-κB activation. The EspC-induced cytokine expression, surface marker upregulation, and MAPK signaling activation were inhibited when macrophages were blocked with anti-TLR4 antibodies or pretreated with MAPK inhibitors. Furthermore, our results showed that EspC overexpression enhanced the survival of M. smegmatis within macrophages and under stress conditions. Taken together, our results indicated that EspC may be another ESX-1 virulence factor that not only modulates the host innate immune response by activating macrophages through TLR4-dependent MAPK signaling but also plays an important role in the survival of pathogenic mycobacteria in host cells

SARS-CoV-2-Specific Adaptive Immunity in COVID-19 Survivors With Asthma

BackgroundAsthma patients potentially have impaired adaptive immunity to virus infection. The levels of SARS-CoV-2-specific adaptive immunity between COVID-19 survivors with and without asthma are presently unclear.MethodsCOVID-19 survivors (patients with asthma n=11, with allergies n=8, and COVID-19 only n=17) and non-COVID-19 individuals (asthmatic patients n=10 and healthy controls n=9) were included. The COVID-19 patients were followed up at about 8 months and 16 months after discharge. The clinical characteristics, lymphocyte subsets, memory T cells, and humoral immunity including SARS-CoV-2 specific antibodies, SARS-CoV-2 pseudotyped virus neutralization assay, and memory B cells were analyzed in these subjects.ResultsThe strength of virus-specific T cell response in COVID-19 survivors was positively correlated with the percentage of blood eosinophils and Treg cells (r=0.4007, p=0.0188; and r=0.4435, p=0.0086 respectively) at 8-month follow-up. There were no statistical differences in the levels of SARS-CoV-2-specific T cell response between the COVID-19 survivors with, and without, asthma. Compared to those without asthma, the COVID-19 with asthma survivors had higher levels of SARS-CoV-2-specific neutralizing antibodies (NAbs) at the 8-month follow-up (p<0.05). Moreover, the level of NAbs in COVID-19 survivors was positively correlated with the percentage of Treg and cTfh2 cells (r=0.5037, p=0.002; and r=0.4846, p=0.0141), and negatively correlated with the percentage of Th1 and Th17 cells (r=-0.5701, p=0.0003; and r=-0.3656, p=0.0308), the ratio of Th1/Th2, Th17/Treg, and cTfh1/cTfh2 cell (r=-0.5356, r=-0.5947, r=-0.4485; all p<0.05). The decay rate of NAbs in the COVID-19 survivors with asthma was not significantly different from that of those without asthma at 16-month follow-up.ConclusionThe level of SARS-CoV-2-specific NAbs in COVID-19 survivors with asthma was higher than that of those without asthma at 8-month follow-up. The SARS-CoV-2-specific T cell immunity was associated with blood eosinophils and Treg percentages. The SARS-CoV-2-specific humoral immunity was closely associated with cTfh2/cTfh1 imbalance and Treg/Th17 ratio. According to the findings, asthmatic patients in COVID-19 convalescent period may benefit from an enhanced specific humoral immunity, which associates with skewed Th2/Th1 and Treg/Th17 immune