The complete mitochondrial genome of Indian Cuckoo Cuculus micropterus (Aves: Cuculiformes)

The Indian Cuckoo, Cuculus micropterus, belongs to the family Cuculidae. In this paper, we sequenced and analysized the complete mitochondrial genome of C. micropterus. The complete mitochondrial genome of C. micropterus is 17,541 bp in length, which was submitted to the NCBI database under the accession number MZ048030. It contains 13 protein-coding genes, 22 transfer RNA genes, two ribosome RNA genes, and one non-coding control region. The overall base composition of the mitochondrial DNA is 33.2% for A, 24.2% for T, 29.8% for C, and 12.8% for G, with a GC content of 42.6%. In order to explore the molecular phylogenetics evolution of Cuculidae, the nucleotide sequence data of 13 PCGs of C. micropterus and other 11 Cuculiformes were used for the phylogenetic analysis. The result shows that C. micropterus is closely related to Cuculus canorus bakeri. The study contributes to illuminating the taxonomic status of C. micropterus, and may facilitate further investigation of the evolution of Cuculidae

Evidence Summary for Nonpharmacological Management of Chemotherapy-Induced Nausea and Vomiting

Objective. To evaluate and summarize the best evidence for nonpharmacological management of chemotherapy induced nausea and vomiting (CINV). This article aims at serving as a reference for nursing staff to prevent and manage CINV. Methods. We systematically searched for evidence on CINV in databases such as Cnki and Wanfang, guide websites such as GIN and NZGG, and professional websites such as UICC and ACS. Only articles published between November 2012 and November 2021 were considered for inclusion in the summary. Two researchers evaluated the quality of the literature and extracted the data. Results. A total of 22 articles were included in this study, including 3 clinical decisions, 2 guidelines, 1 evidence summary, 2 recommended practices, 13 systematic reviews, and 1 expert consensus. Finally, 15 pieces of evidence were summarized from 3 perspectives: risk factor assessment, nursing process specification, and nonpharmacological interventions. Conclusion. Our research summarizes the best evidence on nonpharmacological management of CINV. During actual clinical application, it is necessary to fully consider the clinical situation, combine professional judgment with patients’ wishes, follow the principle of individualization, analyze the obstacles and facilitating factors of the application of evidence, and prudently apply the evidence to the clinical practice

Genome-wide Association Study Identifies Loci for the Polled Phenotype in Yak

<div><p>The absence of horns, known as the polled phenotype, is an economically important trait in modern yak husbandry, but the genomic structure and genetic basis of this phenotype have yet to be discovered. Here, we conducted a genome-wide association study with a panel of 10 horned and 10 polled yaks using whole genome sequencing. We mapped the <i>POLLED</i> locus to a 200-kb interval, which comprises three protein-coding genes. Further characterization of the candidate region showed recent artificial selection signals resulting from the breeding process. We suggest that expressional variations rather than structural variations in protein probably contribute to the polled phenotype. Our results not only represent the first and important step in establishing the genomic structure of the polled region in yak, but also add to our understanding of the polled trait in bovid species.</p></div

Mixed-use building

The aim of my master´s thesis is a design of mixed-use building. The new building is designed to be a permanent resident with offices and café. The objech has one basement and three above floor. In the basement is amenities of house, office premises and café. All the above floor are designed for housing. The object is on sloping terrain on the selected pieces of land in north-west part of city Ceske Budejovice. Structural system is from permanent formwork. The house is roofed with warm flat floor. Drawing part processed in a computer program Archicad

Distribution of population genomic parameters along scaffold526_1.

<p>The plots show: (<b>A</b>) the nucleotide diversity (π, blue for horned and red for polled yaks) for each population; (<b>B</b>) the proportion of shared polymorphisms among sites that are polymorphic in at least one population (green), the proportion of private polymorphisms among sites that are polymorphic within populations (blue for horned and red for polled yaks), private and shared polymorphisms shown in the same panel; (<b>C</b>) <i>F</i>_ST; (<b>D</b>) <i>d</i>_xy; and (<b>E</b>) XP-CLR of scaffold526_1.</p

Additional file 8: of Genome-wide patterns of copy number variation in the Chinese yak genome

Gene ontology annotations for genes covered by whole genome and CNVRs. The left y-axis indicates the percentage of a specific category of genes in a main category, while the right axis indicates the number of genes in it. (PNG 1006 kb

Phylogenetic and population structure of horned and polled yaks.

<p>(<b>A</b>) Photos of horned (above) and polled (below) yak herds, taken by Chunnian Liang. (<b>B</b>) A neighbor-joining phylogenetic tree constructed using whole-genome SNP data. The scale bar represents level of similarity. Horned (blue) and polled (red) samples are indicated. (<b>C</b>) Principal component (PC) analysis plots of the first two components. (<b>D</b>) A neighbor-joining phylogenetic tree constructed using SNP data for the GWAS region.</p

Associated mapping of the polled phenotype.

<p>(<b>A</b>) Genome-wide <i>P</i> values (y axis) are plotted along the genome (<b>B</b>) and magnification of scaffold526_1. (<b>C</b>) All genes around the candidate GWAS region. (<b>D</b>) diagram of read depths (X axes) of RNA-seq data mapping of five different tissues: brain (B), kidney (K), lung (Lu), liver (Li) and heart (H), each with two replicates (Y axes).</p

Haplotype block at linkage disequilibrium along the scaffold526_1.

<p>Haplotype block at linkage disequilibrium along the scaffold526_1.</p