Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA interference

<p>Abstract</p> <p>Background</p> <p>Adenoid cystic carcinoma is one of the most common types of salivary gland cancers. The poor long-term prognosis for patients with adenoid cystic carcinoma is mainly due to local recurrence and distant metastasis. Disintegrin and metalloprotease 10 (ADAM 10) is a transmembrane protein associated with metastasis in a number of diverse of cancers. The aim of this study was to analyze the relationship between ADAM 10 and the invasive and metastatic potentials as well as the proliferation capability of adenoid cystic carcinoma cells <it>in vitro </it>and <it>in vivo</it>.</p> <p>Methods</p> <p>Immunohistochemistry and Western blot analysis were applied to detect ADAM 10 expression levels in metastatic cancer tissues, corresponding primary adenoid cystic carcinoma tissues, adenoid cystic carcinoma cell lines with high metastatic potential, and adenoid cystic carcinoma cell lines with low metastatic potential. RNA interference was used to knockdown ADAM 10 expression in adenoid cystic carcinoma cell lines with high metastatic potential. Furthermore, the invasive and metastatic potentials as well as the proliferation capability of the treated cells were observed <it>in vitro </it>and <it>in vivo</it>.</p> <p>Results</p> <p>It was observed that ADAM 10 was expressed at a significantly higher level in metastatic cancer tissues and in adenoid cystic carcinoma cell lines with high metastatic potential than in corresponding primary adenoid cystic carcinomas and adenoid cystic carcinoma cell lines with low metastatic potential. Additionally, silencing of ADAM 10 resulted in inhibition of cell growth and invasion <it>in vitro </it>as well as inhibition of cancer metastasis in an experimental murine model of lung metastases <it>in vivo</it>.</p> <p>Conclusions</p> <p>These studies suggested that ADAM 10 plays an important role in regulating proliferation and metastasis of adenoid cystic carcinoma cells. ADAM 10 is potentially an important therapeutic target for the prevention of tumor metastases in adenoid cystic carcinoma.</p

Magnetic properties of dashing rocks loess at Timaru, South Island, New Zealand

The relationships between magnetic susceptibility and pedogenic development are different in various regions of the world. For example, loess magnetic susceptibility shows a positive correlation with pedogenic development in Chinese Loess Plateau (CLP),

Magneto-Chemotaxis in Sediment: First Insights

Magnetotactic bacteria (MTB) use passive alignment with the Earth magnetic field as a mean to increase their navigation efficiency in horizontally stratified environments through what is known as magneto-aerotaxis (M-A). Current M-A models have been derived from MTB observations in aqueous environments, where a >80% alignment with inclined magnetic field lines produces a one-dimensional search for optimal living conditions. However, the mean magnetic alignment of MTB in their most widespread living environment, i.e. sediment, has been recently found to be <1%, greatly reducing or even eliminating the magnetotactic advantage deduced for the case of MTB in water. In order to understand the role of magnetotaxis for MTB populations living in sediment, we performed first M-A observations with lake sediment microcosms. Microcosm experiments were based on different combinations of (1) MTB position with respect to their preferred living depth (i.e. above, at, and below),and (2) magnetic field configurations (i.e. correctly and incorrectly polarized vertical fields, horizontal fields, and zero fields). Results suggest that polar magnetotaxis is more complex than implied by previous experiments, and revealed unexpected differences between two types of MTB living in the same sediment. Our main findings are: (1) all investigated MTB benefit of a clear magnetotactic advantage when they need to migrate over macroscopic distances for reaching their optimal living depth,(2) magnetotaxis is not used by all MTB under stationary, undisturbed conditions,(3) some MTB can rely only on chemotaxis for macroscopic vertical displacements in sediment while other cannot, and (4) some MTB use a fixed polar M-A mechanisms, while other can switch their M-A polarity, performing what can be considered as a mixed polar-axial M-A. These observations demonstrate that sedimentary M-A is controlled by complex mechanical, chemical, and temporal factors that are poorly reproduced in aqueous environments

A numerical study of axisymmetric wave propagation in buried fluid-filled pipes for optimizing the vibro-acoustic technique when locating gas pipelines

Buried pipeline systems play a vital role in energy storage and transportation, especially for fluid energies like water and gas. The ability to locate buried pipes is of great importance since it is fundamental for leakage detection, pipeline maintenance, and pipeline repair. The vibro-acoustic locating method, as one of the most effective detection technologies, has been studied by many researchers. However, previous studies have mainly focused on vibro-acoustic propagation in buried water pipes. Limited research has been conducted on buried gas pipes. In this paper, the behavior of gas-dominated wave motion will be investigated and compared against water-dominated wave motion by adapting an established analytical model of axisymmetric wave motion in buried fluid-filled pipes. Furthermore, displacement profiles in spatial domain resulting from gas-dominated wave in buried gas pipeline systems will be analyzed, and the effects of pipe material, soil property, as well as mode wave type will be discussed in detail. An effective radiation coefficient (ERC) is proposed to measure the effective radiation ability of gas-dominated wave and water-dominated wave. It is observed that the gas-dominated wave in gas pipes cannot radiate into surrounded soil as effectively as water-dominated wave in water pipes because of the weak coupling between gas and pipe-soil. In this case, gas-dominated wave may not be the best choice as the target wave for locating buried gas pipes. Therefore, the soil displacements result from the shell-dominated wave are also investigated and compared with those from gas-dominated wave. The results show that for buried gas pipes, the soil displacements due to radiation of shell-dominated wave are stronger than gas-dominated wave, which differs from buried water pipe. Hence, an effectively exciting shell-dominated wave is beneficial for generating stronger vibration signals and obtaining the location information. The findings of this study provide theoretical insight for optimizing the current vibro-acoustic method when locating buried gas pipes

A Review of Static Pressure Reset Control in Variable Air Volume Air Condition System

AbstractFor the sake of energy saving of variable air volume (VAV) system, this paper presents the a review of static pressure control around the optimization problem of static pressure reset in VAV air conditioning system. Then, main control methods of static pressure reset are described, and existing problems are analyzed and concluded. Finally, it is pointed out that the critical technology and the development trend of static pressure reset control. This overview is not intended to be an exhaustive survey on this topic, and any omission of other works is purely unintentional

A comprehensive review of acoustic methods for locating underground pipelines

Underground pipelines are vital means of transporting fluid resources like water, oil and gas. The process of locating buried pipelines of interest is an essential prerequisite for pipeline maintenance and repair. Acoustic pipe localization methods, as effective trenchless detection techniques, have been implemented in locating underground utilities and shown to be very promising in plastic pipeline localization. This paper presents a comprehensive review of current acoustic methods and recent advances in the localization of buried pipelines. Investigations are conducted from multiple perspectives including the wave propagation mechanism in buried pipe systems, the principles behind each method along with advantages and limitations, representative acoustic locators in commercial markets, the condition of buried pipes, as well as selection of preferred methods for locating pipelines based on the applicability of existing localization techniques. In addition, the key features of each method are summarized and suggestions for future work are proposed. Acoustic methods for locating underground pipelines have proven to be useful and effective supplements to existing localization techniques. It has been highlighted that the ability of acoustic methods to locate non-metallic objects should be of particular practical value. While this paper focuses on a specific application associated with pipeline localization, many acoustic methods are feasible across a wide range of underground infrastructures