197 research outputs found
Genome sequence and organization of a nucleopolyhedrovirus that infects the tea looper caterpillar, Ectropis obliqua
AbstractThe complete nucleotide sequence of Ectropis obliqua nucleopolyhedrovirus (EcobNPV), which infects the tea looper caterpillar, was determined and analyzed. The double stranded circular genome is composed of 131,204 bp and is 37.6% G+C rich. The analysis predicted 126 putative, minimally overlapping open reading frames (ORFs) with 150 or more nucleotides that together compose 89.8% of the genome. The remaining 10.2% constitute non-coding and three homologous regions. Comparison with previously sequenced baculoviruses indicated that three ORFs were unique to EcobNPV, while the remaining 123 ORFs shared identity with other baculovirus genes. In addition to two bro homologues, three other repeat ORFs, including dbp, p26, and odv-e66, were identified. Phylogenetic analysis indicated that each member of the paired ORFs was acquired independently. Gene parity plot analysis and percent identity of gene homologues suggested that EcobNPV is a Group II NPV, although its genomic organization was highly distinct
Poly[[aqua-μ6-benzene-1,2,3-tricarboxylato-μ3-hydroxido-dizinc] hemihydrate]
In the title compound, {[Zn2(C9H3O6)(OH)(H2O)]·0.5H2O}n, there are three independent ZnII atoms present; two are located on special positions, viz a twofold rotation axis and an inversion centre, and the third is located in a general position. The ZnII atom on the inversion centre is six-coordinated by four O atoms from four different benzene-1,2,3-tricarboxylate anions and two OH− anions. The ZnII atom located on a twofold axis is four coordinated by two O atoms from two different benzene-1,2,3-tricarboxylate anions and two OH− anions. The third ZnII atom, located in a general position, is five coordinated by three O atoms from three different benzene-1,2,3-tricarboxylate anions, one OH− anion and one water molecule. Each benzene-1,2,3-tricarboxylate anion bridges six ZnII atoms, and the OH− anion bridges three ZnII atoms, resulting in the formation of a three-dimensional framework. A series of O—H⋯O hydrogen bonds involving the benzene-1,2,3-tricarboxylate anions, the OH− anion and the coordinating and the two water solvent molecules further stablize the crystal structure. The two solvent water molecules show occupancies of 0.5 and 0.25
Improved Spatial Resolution Achieved by Chromatic Intensity Interferometry
Interferometers are widely used in imaging technologies to achieve enhanced
spatial resolution, but require that the incoming photons be indistinguishable.
In previous work, we built and analyzed color erasure detectors which expand
the scope of intensity interferometry to accommodate sources of different
colors. Here we experimentally demonstrate how color erasure detectors can
achieve improved spatial resolution in an imaging task, well beyond the
diffraction limit. Utilizing two 10.9 mm-aperture telescopes and a 0.8 m
baseline, we measure the distance between a 1063.6 nm source and a 1064.4 nm
source separated by 4.2 mm at a distance of 1.43 km, which surpasses the
diffraction limit of a single telescope by about 40 times. Moreover, chromatic
intensity interferometry allows us to recover the phase of the Fourier
transform of the imaged objects - a quantity that is, in the presence of modest
noise, inaccessible to conventional intensity interferometry.Comment: 5 pages, 3 figure
Risk factor analysis for major mediastinal vessel invasion in thymic epithelial tumors based on multi-slice CT Imaging
ObjectiveTo explore the characteristics and risk factors for major mediastinal vessel invasion in different risk grades of thymic epithelial tumors (TETs) based on computed tomography (CT) imaging, and to develop prediction models of major mediastinal artery and vein invasion.MethodsOne hundred and twenty-two TET patients confirmed by histopathological analysis who underwent thorax CT were enrolled in this study. Clinical and CT data were retrospectively reviewed for these patients. According to the abutment degree between the tumor and major mediastinal vessels, the arterial invasion was divided into grade I, II, and III (< 25%, 25 – 49%, and ≥ 50%, respectively); the venous invasion was divided into grade I and II (< 50% and ≥ 50%). The degree of vessel invasion was compared among different defined subtypes or stages of TETs using the chi-square tests. The risk factors associated with TET vascular invasion were identified using multivariate logistic regression analysis.ResultsBased on logistic regression analysis, male patients (β = 1.549; odds ratio, 4.824) and the pericardium or pleural invasion (β = 2.209; odds ratio, 9.110) were independent predictors of 25% artery invasion, and the midline location (β = 2.504; odds ratio, 12.234) and mediastinal lymphadenopathy (β = 2.490; odds ratio, 12.06) were independent predictors of 50% artery invasion. As for 50% venous invasion, the risk factors include midline location (β = 2.303; odds ratio, 10.0), maximum tumor diameter larger than 5.9 cm (β = 4.038; odds ratio, 56.736), and pericardial or pleural effusion (β = 1.460; odds ratio, 4.306). The multivariate logistic model obtained relatively high predicting efficacy, and the area under the curve (AUC), sensitivity, and specificity were 0.944, 84.6%, and 91.7% for predicting 50% artery invasion, and 0.913, 81.8%, and 86.0% for 50% venous invasion in TET patients, respectively.ConclusionSeveral CT features can be used as independent predictors of ≥50% artery or venous invasion. A multivariate logistic regression model based on CT features is helpful in predicting the vascular invasion grades in patients with TET
Chromatic interferometry with small frequency differences
By developing a `two-crystal' method for color erasure, we can broaden the
scope of chromatic interferometry to include optical photons whose frequency
difference falls outside of the 400 nm to 4500 nm wavelength range, which is
the passband of a PPLN crystal. We demonstrate this possibility experimentally,
by observing interference patterns between sources at 1064.4 nm and 1063.6 nm,
corresponding to a frequency difference of about 200 GHz.Comment: 5 pages, 3 figure
Molecular footprints of domestication and improvement in soybean revealed by whole genome re-sequencing
BACKGROUND: Artificial selection played an important role in the origin of modern Glycine max cultivars from the wild soybean Glycine soja. To elucidate the consequences of artificial selection accompanying the domestication and modern improvement of soybean, 25 new and 30 published whole-genome re-sequencing accessions, which represent wild, domesticated landrace, and Chinese elite soybean populations were analyzed. RESULTS: A total of 5,102,244 single nucleotide polymorphisms (SNPs) and 707,969 insertion/deletions were identified. Among the SNPs detected, 25.5% were not described previously. We found that artificial selection during domestication led to more pronounced reduction in the genetic diversity of soybean than the switch from landraces to elite cultivars. Only a small proportion (2.99%) of the whole genomic regions appear to be affected by artificial selection for preferred agricultural traits. The selection regions were not distributed randomly or uniformly throughout the genome. Instead, clusters of selection hotspots in certain genomic regions were observed. Moreover, a set of candidate genes (4.38% of the total annotated genes) significantly affected by selection underlying soybean domestication and genetic improvement were identified. CONCLUSIONS: Given the uniqueness of the soybean germplasm sequenced, this study drew a clear picture of human-mediated evolution of the soybean genomes. The genomic resources and information provided by this study would also facilitate the discovery of genes/loci underlying agronomically important traits
Asthma Pregnancy Alters Postnatal Development of Chromaffin Cells in the Rat Adrenal Medulla
Background: Adrenal neuroendocrine plays an important role in asthma. The activity of the sympathoadrenal system could be altered by early life events. The effects of maternal asthma during pregnancy on the adrenal medulla of offspring remain unknown. Methodology/Principal Findings: This study aims to explore the influence of maternal asthma during pregnancy on the development and function of adrenal medulla in offspring from postnatal day 3 (P3) to postnatal day 60 (P60). Asthmatic pregnant rats (AP), nerve growth factor (NGF)-treated pregnant rats (NP) and NGF antibody-treated pregnant rats (ANP) were sensitized and challenged with ovalbumin (OVA); NP and ANP were treated with NGF and NGF antibody respectively. Offspring rats from the maternal group were divided into four groups: offspring from control pregnant rats (OCP), offspring from AP (OAP), offspring from NP (ONP), and offspring from ANP (OANP). The expressions of phenylethanolamine N-methyltransferase (PNMT) protein in adrenal medulla were analyzed. The concentrations of epinephrine (EPI), corticosterone and NGF in serum were measured. Adrenal medulla chromaffin cells (AMCC) were prone to differentiate into sympathetic nerve cells in OAP and ONP. Both EPI and PNMT were decreased in OAP from P3 to P14, and then reached normal level gradually from P30 to P60, which were lower from birth to adulthood in ONP. Corticosterone concentration increased significantly in OAP and ONP. Conclusion/Significance: Asthma pregnancy may promote AMCC to differentiate into sympathetic neurons in offspring rats and inhibit the synthesis of EPI, resulting in dysfunction of bronchial relaxation
- …