15 research outputs found
Morphology of Novel Chitosan Oligosaccharides Mod-ified Cross-linked Polyvinyl Alcohol Complex Foam (COS/cPVACF) Dressings with Fully Open-cell and Open-channel Microstructures via Active Molecules Cleaning Process for Wound Managements
The design of medical devices could be applied and developed for new treatment proce-dures instead of the traditional therapy procedures
Preclinical Evaluations of Deigned Injectable Decellularized Extracellular Matrix Collagen Micro-particles as Scaffolds via A Combination Process with Supercritical Carbon Dioxide for Injection Knee Injury Repair
Preclinical Evaluations of Deigned Injectable Decellularized Extracellular Matrix Collagen Micro-particles as Scaffolds via A Combination Process with Supercritical Carbon Dioxide for Injection Knee Injury Repair
Biological evaluations of novel surgical complex sponges with type I/type III collagens via electrospray ionization mass spectrometry and quantitative detections
Biological evaluations of novel surgical complex sponges with type I/type III collagens via electrospray ionization mass spectrometry and quantitative detections
Preclinical evaluations and morphology of deigned collagen III/I complex dressings with ECM microstructure derived from a combination procedure with supercritical carbon dioxide fluid process for diabetic foot ulcer wound managements
Preclinical evaluations and morphology of deigned collagen III/I complex dressings with ECM microstructure derived from a combination procedure with supercritical carbon dioxide fluid process for diabetic foot ulcer wound managements
Preclinical evaluations of novel controlled release transdermal delivery system derived from high permeability cross-linked polyvinyl alcohol foam with fully open-cell and open-channel microstructures for diabetic foot ulcer wound managements
Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells
The use of various types of calcium phosphate has been reported in the preparation of repairing materials for bone defects. However, the physicochemical and biological properties among them might be vastly different. In this study, we prepared two types of calcium phosphates, nano-hydroxyapatite (nHA) and natural bone ceramic (NBC), into 3D scaffolds by mixing with type I collagen (CoL), resulting in the nHA/CoL and NBC/CoL scaffolds. We then evaluated and compared the physicochemical and biological properties of these two calcium phosphates and their composite scaffold with CoL. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD) and compressive tests were used to, respectively, characterize the morphology, composition, distribution and the effect of nHA and NBC to collagen. Next, we examined the biological properties of the scaffolds using cytotoxicity testing, flow cytometry, immunofluorescence staining, biocompatibility testing, CCK-8 assays and RT-PCR. The results reflected that the Ca2+ released from nHA and NBC could bind chemically with collagen and affect its physicochemical properties, including the infrared absorption spectrum and compression modulus, among others. Furthermore, the two kinds of scaffolds could promote the expression of osteo-relative genes, but showed different gene induction properties. In short, NBC/CoL could promote the expression of early osteogenic genes, while nHA/CoL could upregulate late osteogenic genes. Conclusively, these two composite scaffolds could provide MC3T3-E1 cells with a biomimetic surface for adhesion, proliferation and the formation of mineralized extracellular matrices. Moreover, nHA/CoL and NBC/CoL had different effects on the period and extent ofMC3T3-E1 cell mineralization