Hegels Begriff der "eigentlichen Metaphysik"

Woran liegt es, dass trotz Hegels Behauptung, die spekulative Logik sei die eigentliche Metaphysik, ein Zwiespalt zwischen der metaphysischen Lesart und der nicht-metaphysischen bzw. transzendentalphilosophischen Lesart der Wissenschaft der Logik in der Hegelforschung zum Vorschein gekommen ist? Was für eine systematische Untersuchung der Wissenschaft der Logik wird methodologisch Hegels metaphysischem Anspruch gerecht? Anhand eines Überblicks über Hegels gesamte logische und enzyklopädische Philosophie versucht Zhili Xiong sowohl eine begleitende Lektüre für Hegel-Anfänger als auch eine erweiterte epistemische Lesart der Hegelschen Metaphysik für Hegel-Kenner anzubieten

DREAMPlaceFPGA-MP: An Open-Source GPU-Accelerated Macro Placer for Modern FPGAs with Cascade Shapes and Region Constraints

FPGA macro placement plays a pivotal role in routability and timing closer to the modern FPGA physical design flow. In modern FPGAs, macros could be subject to complex cascade shape constraints requiring instances to be placed in consecutive sites. In addition, in real-world FPGA macro placement scenarios, designs could have various region constraints that specify boundaries within which certain design instances and macros should be placed. In this work, we present DREAMPlaceFPGA-MP, an open-source GPU-accelerated FPGA macro-placer that efficiently generates legal placements for macros while honoring cascade shape requirements and region constraints. Treating multiple macros in a cascade shape as a large single instance and restricting instances to their respective regions, DREAMPlaceFPGA-MP obtains roughly legal placements. The macros are legalized in multiple steps to efficiently handle cascade shapes and region constraints. Our experimental results demonstrate that DREAMPlaceFPGA-MP is among the top contestants of the MLCAD 2023 FPGA Macro-Placement Contest

hSef potentiates EGF-mediated MAPK signaling through affecting EGFR trafficking and degradation

Sef (similar expression to fgf genes) was identified as an effective antagonist of fibroblast growth factor (FGF) in vertebrates. Previous reports have demonstrated that Sef interacts with FGF receptors (FGFRs) and inhibits FGF signaling, however, its role in regulating epidermal growth factor receptor (EGFR) signaling remains unclear. In this report, we found that hSef localizes to the plasma membrane (PM) and is subjected to rapid internalization and well localizes in early/recycling endosomes while poorly in late endosomes/lysosomes. We observed that hSef interacts and functionally colocalizes with EGFR in early endosomes in response to EGF stimulation. Importantly, we demonstrated that overexpression of hSef attenuates EGFR degradation and potentiates EGF-mediated mitogen-activated protein kinase (MAPK) signaling by interfering EGFR trafficking. Finally, our data showed that, with overexpression of hSef, elevated levels of Erk phosphorylation and differentiation of rat pheochromocytoma (PC12) cells occur in response to EGF stimulation. Taken together, these data suggest that hSef plays a positive role in the EGFR-mediated MAPK signaling pathway. This report, for the first time, reveals opposite roles for Sef in EGF and FGF signalings

Assessing the Microbial Community and Functional Genes in a Vertical Soil Profile with Long-Term Arsenic Contamination

Conceived and designed the experiments: GW. Performed the experiments: JX GL. Analyzed the data: JX JZ GW. Contributed reagents/materials/analysis tools: ST JZ GW. Wrote the paper: JX ZH JDVN JZ GW.Arsenic (As) contamination in soil and groundwater has become a serious problem to public health. To examine how microbial communities and functional genes respond to long-term arsenic contamination in vertical soil profile, soil samples were collected from the surface to the depth of 4 m (with an interval of 1 m) after 16-year arsenic downward infiltration. Integrating BioLog and functional gene microarray (GeoChip 3.0) technologies, we showed that microbial metabolic potential and diversity substantially decreased, and community structure was markedly distinct along the depth. Variations in microbial community functional genes, including genes responsible for As resistance, carbon and nitrogen cycling, phosphorus utilization and cytochrome c oxidases were detected. In particular, changes in community structures and activities were correlated with the biogeochemical features along the vertical soil profile when using the rbcL and nifH genes as biomarkers, evident for a gradual transition from aerobic to anaerobic lifestyles. The C/N showed marginally significant correlations with arsenic resistance (p = 0.069) and carbon cycling genes (p = 0.073), and significant correlation with nitrogen fixation genes (p = 0.024). The combination of C/N, NO3− and P showed the highest correlation (r = 0.779, p = 0.062) with the microbial community structure. Contradict to our hypotheses, a long-term arsenic downward infiltration was not the primary factor, while the spatial isolation and nutrient availability were the key forces in shaping the community structure. This study provides new insights about the heterogeneity of microbial community metabolic potential and future biodiversity preservation for arsenic bioremediation management.Yeshttp://www.plosone.org/static/editorial#pee

How sulphate-reducing microorganisms cope with stress: lessons from systems biology

Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery

Applications of Carbon Nanotubes for Lithium Ion Battery Anodes

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Simultaneous determination of seven constituents in Si-Ni-San decoction and a compatibility comparison study using HPLC–UV

<div><p>A simple and accurate HPLC–UV method was developed for simultaneous determination of seven constituents in Si-Ni-San decoction. Separation was performed on a Hypersil C₁₈ column and detection was set with gradient wavelength at 240 nm (0–26 min) and 210 nm (26–30 min). The mobile phase consisted of 0.03% phosphoric acid in water (v/v), and acetonitrile was used with a flow rate of 1.0 ^− 1. This method provides good linearity (<i>r</i>>0.9992), precision (RSD < 1.9%), repeatability (RSD < 2.9%), stability (RSD < 2.9%) and recovery (97.6–102.0%), which has been successfully applied to quantitative determination of the seven constituents in Si-Ni-San decoction and different compatibility groups.</p></div

A Hydrothermal Method to Generate Carbon Quantum Dots from Waste Bones and Their Detection of Laundry Powder

Surfactants are one of the major pollutants in laundry powder, which have an impact on the environment and human health. Carbon quantum dots (CQDs) are spherical zero-dimensional fluorescent nanoparticles with great potential for fluorescent probing, electrochemical biosensing and ion sensing. Herein, a bottom-up approach was developed for the synthesis of CQDs from biomass to detect laundry detergent and laundry powder. Waste chicken bones were used as carbon precursors after being dried, crushed and reacted with pure water at 180 &deg;C for 4 h to generate CQDs, which exhibited a monodisperse quasi-spherical structure with an average particle size of 3.2 &plusmn; 0.2 nm. Functional groups, including -OH, C=O, C=C and C-O, were identified on the surface of the prepared CQDs. The optimal fluorescence excitation wavelength of the yellow-brown CQDs was 380 nm, with a corresponding emission peak at 465 nm. CQDs did not significantly increase cell death in multiple cell lines at concentrations of 200 &micro;g&middot;mL&minus;1. Fluorescence enhancement of CQDs was observed after addition of sodium dodecyl benzene sulphonate, a major anionic surfactant in laundry powder. A linear relationship between fluorescence enhancement CQDs and the concentration of laundry powder was established. Thus, a hydrothermal method was developed to generate CQDs from waste biomass that may be used as a fluorescent probe to detect laundry powder

Simultaneous determination of six constituents in the fruit of <i>Acanthopanax sessiliflorus</i> (Rupr. et Maxim.) Seem. by HPLC–UV

<div><p>A simple and accurate liquid chromatographic method was developed for the simultaneous determination of six constituents in the fruit of <i>Acanthopanax sessiliflorus</i>. The conditions of sample extraction were optimised by using orthogonal design. The method provided good accuracy with recoveries in the range of 95.6–101.6% and good precision with RSD values less than 3.0%, which has been successfully applied to the quantitative determination of the six compounds in the fruit of <i>A</i>.<i> sessiliflorus</i> from two maturation periods.</p></div