Evaluation of Masters Choice Corn Silage on Growing Steer Performance

A growing study evaluated three corn silage hybrids on growing steer performance. Th e three hybrids were: a conventional hybrid- Farm Choice (CON) commonly grown in Eastern Nebraska which served as the control, Masters Choice hybrids MCT6365 RIB (MC1) selected to improve fi ber and starch digestion and MCT6733 GT3000 (MC2) that has been selected to improve fi ber digestion in cattle. Relative to CON, feeding hybrid MC1 resulted in similar DMI, but numerically increased ADG which significantly improved F:G compared to CON. Feeding MC2 led to greater DMI, similar ADG, and poorer (greater) F:G compared to CON. Feeding Masters Choice hybrid MCT6365 RIB (MC1) corn silage at 80% of the diet DM likely improved digestion and energy availability to the steers, which allowed greater ADG and improved F:G, while the opposite was true for MC2. Differences in hybrids exist when fed to growing cattle at 80% of the diet

Evaluation of Masters Choice Corn Silage on Growing Steer Performance

A growing study evaluated three corn silage hybrids on growing steer performance. Th e three hybrids were: a conventional hybrid- Farm Choice (CON) commonly grown in Eastern Nebraska which served as the control, Masters Choice hybrids MCT6365 RIB (MC1) selected to improve fi ber and starch digestion and MCT6733 GT3000 (MC2) that has been selected to improve fi ber digestion in cattle. Relative to CON, feeding hybrid MC1 resulted in similar DMI, but numerically increased ADG which significantly improved F:G compared to CON. Feeding MC2 led to greater DMI, similar ADG, and poorer (greater) F:G compared to CON. Feeding Masters Choice hybrid MCT6365 RIB (MC1) corn silage at 80% of the diet DM likely improved digestion and energy availability to the steers, which allowed greater ADG and improved F:G, while the opposite was true for MC2. Differences in hybrids exist when fed to growing cattle at 80% of the diet

Tumor-Derived Exosomal Protein Tyrosine Phosphatase Receptor Type O Polarizes Macrophage to Suppress Breast Tumor Cell Invasion and Migration

Tumor-derived exosomes, containing multiple nucleic acids and proteins, have been implicated to participate in the interaction between tumor cells and microenvironment. However, the functional involvement of phosphatases in tumor-derived exosomes is not fully understood. We and others previously demonstrated that protein tyrosine phosphatase receptor type O (PTPRO) acts as a tumor suppressor in multiple cancer types. In addition, its role in tumor immune microenvironment remains elusive. Bioinformatical analyses revealed that PTPRO was closely associated with immune infiltration, and positively correlated to M1-like macrophages, but negatively correlated to M2-like macrophages in breast cancer tissues. Co-cultured with PTPRO-overexpressing breast cancer cells increased the proportion of M1-like tumor-associated macrophages (TAMs) while decreased that of M2-like TAMs. Further, we observed that tumor-derived exosomal PTPRO induced M1-like macrophage polarization, and regulated the corresponding functional phenotypes. Moreover, tumor cell-derived exosomal PTPRO inhibited breast cancer cell invasion and migration, and inactivated STAT signaling in macrophages. Our data suggested that exosomal PTPRO inhibited breast cancer invasion and migration by modulating macrophage polarization. Anti-tumoral effect of exosomal PTPRO was mediated by inactivating STAT family in macrophages. These findings highlight a novel mechanism of tumor invasion regulated by tumor-derived exosomal tyrosine phosphatase, which is of translational potential for the therapeutic strategy against breast cancer

Evaluation of Corn Hybrids Selected to Improve Silage Use by Growing Steers and Characterizing Digestion of Novel Sweet Bran Plus Products from Corn Wet Milling

Three studies were conducted to evaluate corn silage hybrids selected for improved fiber and starch digestion on growing steer performance and nutrient digestion, and to characterize digestion traits of novel sweet bran plus products from corn wet milling. In experiment 1, diets consisted of 80% DM of corn silage hybrid Masters Choice MCT6365 RIB (MC1, selected to improve fiber and starch digestion), MCT6733 GT3000 (MC2, selected to improve fiber digestion), and Pioneer® 1197AM (CON, a conventional corn hybrid commonly grown in Eastern Nebraska) were compared in an 84-day cattle growing study. In experiment 2, the same diets were used to evaluate rumen fermentation and nutrient digestion. Experiment 3 evaluated three novel Sweet Bran Plus products (A, B, and C) from corn wet milling on rumen fermentation, nutrient digestion, and rumen bacterial community structure in beef cattle. In experiment 1 and 2, feeding MC1 corn silage improved organic matter digestion (P = 0.02), starch digestion (P \u3c 0.01), and energy availability (P ≤ 0.07) to the steers, which allowed for greater average daily gain (P = 0.09) and improved feed efficiency (P \u3c 0.01). In experiment 3, feeding Sweet Bran Plus A resulted in better digestion of starch and NDF than product B and C, and greater starch content of product B and C resulted in less acetate to propionate ratio and less digestion of starch and NDF. Sweet Bran Plus products had minimum effect on energy digestion, ruminal pH, rumination and blood parameters. Rumen bacterial microbiota were altered by different Sweet Bran Plus products and inclusion. Digestion trait differences existed among different corn hybrids, as well as among different sweet bran plus products. Further evaluation is necessary to improve their use in the cattle industry

Impact of Masters Choice Corn Silage on Nutrient Digestion in Growing Cattle

A digestion study was conducted to evaluate Masters Choice corn silage hybrids on nutrient digestibility in growing beef steers. ! e three hybrids evaluated were a conventional hybrid (CON) commonly grown in Eastern Nebraska which served as the control, Masters Choice hybrid MCT6365 RIB (MC1) that has been selected to improve ber and starch digestion and Masters Choice hybrid MCT6733 GT3000 (MC2) selected to improve ber digestion. Treatment diets consisted of 80% of the diet dry matter (DM) of each corn silage hybrid. Steers fed MC1 corn silage had the greatest organic matter (OM), energy digestibility, and digestible energy (DE) content of the diet. Feeding MC2 resulted in the lowest OM, starch, and energy digestibility and dietary DE content. Steer energy digestion (OM, DE) was intermediate to MC1 and MC2 for CON silage. Results indicated that feeding MC1 corn silage at 80% of the diet DM improved digestion and energy availability to the steers, which allowed greater average daily gain and improved feed conversion observed in the corresponding growing trial, while the opposite was true for MC2

Sandwich-like, stacked ultrathin titanate nanosheets for ultrafast lithium storage

Sandwich-like stacked titanate nanosheets with the thickness of ≈0.4 nm are successfully synthesized on a large scale using a clean and facile method. The unique material exhibits superior capacity and excellent high rate performance for ultrafast lithium storage

Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China

Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9%) sera of A. agrarius and 1 of 13 (7.7%) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China