Molecular variance (AMOVA) analysis among different ploidy populations according to rDNA-ITS haplotype data.

<p>Molecular variance (AMOVA) analysis among different ploidy populations according to rDNA-ITS haplotype data.</p

The analysis of variable sites of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.

<p>The analysis of variable sites of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.</p

The ML and NJ phylogenetic tree based on rDNA-ITS haplotype data of different polyploid clones of <i>S</i>. <i>spontaneum</i>.

<p>The ML and NJ phylogenetic tree based on rDNA-ITS haplotype data of different polyploid clones of <i>S</i>. <i>spontaneum</i>.</p

The GC content analysis of composition of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.

<p>The GC content analysis of composition of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.</p

The list of control rDNA-ITS sequences.

<p>The list of control rDNA-ITS sequences.</p

Pairwise Gst (above the diagonal) and Nm (below the diagonal) among different ploidy populations according to rDNA-ITS data.

<p>Pairwise Gst (above the diagonal) and Nm (below the diagonal) among different ploidy populations according to rDNA-ITS data.</p

The T test of genetic distance difference between inter-population and intra-population obtained using Kimura 2-parameter model.

<p>The T test of genetic distance difference between inter-population and intra-population obtained using Kimura 2-parameter model.</p

The list of clones of <i>S</i>. <i>spontaneum</i> used in this study.

<p>The list of clones of <i>S</i>. <i>spontaneum</i> used in this study.</p

Plant materials for cloning and sequencing of nrDNA-ITS sequences.

<p>Plant materials for cloning and sequencing of nrDNA-ITS sequences.</p

Electrophoretogram of nrDNA-ITS PCR products.

<p>M: 100 bp DNA ladder marker. Lanes 1–5: Badila, VN cattle cane, Loethers, Crystalina, Cheribon, respectively, belong to <i>S</i>. <i>officinarum</i>. Lanes 6–10: 57NG208, 51NG63, NG77-004, 28NG21, Daye, respectively, belong to <i>S</i>. <i>robustum</i>. Lanes 11–20: YN75-2-11, YN82-110, YN83-160, FJ89-1-1, YN83-201, YN82-44, YN83-171, GZ78-2-28, FJ88-1-13, FJ89-1-19, respectively, belong to <i>S</i>. <i>spontaneum</i>.</p