Comparative transcriptomics and metabolomics reveal specialized metabolite drought stress responses in switchgrass (<i>Panicum virgatum</i> L.)

⋅ Switchgrass (Panicum virgatum) is a bioenergy model crop valued for its energy efficiency and drought tolerance resilience. The related monocot species rice (Oryza sativa) and maize (Zea mays) deploy species-specific, specialized metabolites as core stress defenses. By contrast, specialized chemical defenses in switchgrass are largely unknown. ⋅ To investigate specialized metabolic drought responses in switchgrass, we integrated tissue-specific transcriptome and metabolite analyses of the genotypes Alamo and Cave-in-Rock that feature different drought tolerance. ⋅ The more drought-susceptible Cave-in-Rock featured an earlier onset of transcriptomic changes and significantly more differentially expressed genes in response to drought compared to Alamo. Specialized pathways showed moderate differential expression compared to pronounced transcriptomic alterations in carbohydrate and amino acid metabolism. However, diterpenoid-biosynthetic genes showed drought-inducible expression in Alamo roots, contrasting largely unaltered triterpenoid and phenylpropanoid pathways. Metabolomic analyses identified common and genotype-specific flavonoids and terpenoids. Consistent with transcriptomic alterations, several root diterpenoids showed significant drought-induced accumulation, whereas triterpenoid abundance remained predominantly unchanged. Structural analysis of drought-responsive root diterpenoids verified these metabolites as oxygenated furanoditerpenoids. ⋅ Drought-dependent transcriptome and metabolite profiles provide the foundation to understand the molecular mechanisms underlying switchgrass environmental resilience. Accumulation of specialized root diterpenoids and corresponding pathway transcripts supports a role in drought stress tolerance for these compounds

Application of a novel phage LPSEYT for biological control of Salmonella in foods

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Salmonella is a leading cause of foodborne diseases, and in recent years, many isolates have exhibited a high level of antibiotic resistance, which has led to huge pressures on public health. Phages are a promising strategy to control food‐borne pathogens. In this study, one of our environmental phage isolates, LPSEYT, was to be able to restrict the growth of zoonotic Salmonella enterica in vitro over a range of multiplicity of infections. Phage LPSEYT exhibited wide‐ranging pH and thermal stability and rapid reproductive activity with a short latent period and a large burst size. Phage LPSEYT demonstrated potential efficiency as a biological control agent against Salmonella in a variety of food matrices, including milk and lettuce. Morphological observation, comparative genomic, and phylogenetic analysis revealed that LPSEYT does not belong to any of the currently identified genera within the Myoviridae family, and we suggest that LPSEYT represents a new genus, the LPSEYTvirus. This study contributes a phage database, develops beneficial phage resources, and sheds light on the potential application value of phages LPSEYT on food safety

Variation and stability of rhizosphere bacterial communities of Cucumis crops in association with root-knot nematodes infestation

IntroductionRoot-knot nematodes (RKN) disease is a devastating disease in Cucumis crops production. Existing studies have shown that resistant and susceptible crops are enriched with different rhizosphere microorganisms, and microorganisms enriched in resistant crops can antagonize pathogenic bacteria. However, the characteristics of rhizosphere microbial communities of Cucumis crops after RKN infestation remain largely unknown.MethodsIn this study, we compared the changes in rhizosphere bacterial communities between highly RKN-resistant Cucumis metuliferus (cm3) and highly RKN-susceptible Cucumis sativus (cuc) after RKN infection through a pot experiment. ResultsThe results showed that the strongest response of rhizosphere bacterial communities of Cucumis crops to RKN infestation occurred during early growth, as evidenced by changes in species diversity and community composition. However, the more stable structure of the rhizosphere bacterial community in cm3 was reflected in less changes in species diversity and community composition after RKN infestation, forming a more complex and positively co-occurrence network than cuc. Moreover, we observed that both cm3 and cuc recruited bacteria after RKN infestation, but the bacteria enriched in cm3 were more abundant including beneficial bacteria Acidobacteria, Nocardioidaceae and Sphingomonadales. In addition, the cuc was enriched with beneficial bacteria Actinobacteria, Bacilli and Cyanobacteria. We also found that more antagonistic bacteria than cuc were screened in cm3 after RKN infestation and most of them were Pseudomonas (Proteobacteria, Pseudomonadaceae), and Proteobacteria were also enriched in cm3 after RKN infestation. We hypothesized that the cooperation between Pseudomonas and the beneficial bacteria in cm3 could inhibit the infestation of RKN.DiscussionThus, our results provide valuable insights into the role of rhizosphere bacterial communities on RKN diseases of Cucumis crops, and further studies are needed to clarify the bacterial communities that suppress RKN in Cucumis crops rhizosphere

HMGB1: a double-edged sword and therapeutic target in the female reproductive system

HMGB1 that belongs to the High Mobility Group-box superfamily, is a nonhistone chromatin associated transcription factor. It is present in the nucleus of eukaryotes and can be actively secreted or passively released by kinds of cells. HMGB1 is important for maintaining DNA structure by binding to DNA and histones, protecting it from damage. It also regulates the interaction between histones and DNA, affecting chromatin packaging, and can influence gene expression by promoting nucleosome sliding. And as a DAMP, HMGB1 binding to RAGE and TLRs activates NF-κB, which triggers the expression of downstream genes like IL-18, IL-1β, and TNF-α. HMGB1 is known to be involved in numerous physiological and pathological processes. Recent studies have demonstrated the significance of HMGB1 as DAMPs in the female reproductive system. These findings have shed light on the potential role of HMGB1 in the pathogenesis of diseases in female reproductive system and the possibilities of HMGB1-targeted therapies for treating them. Such therapies can help reduce inflammation and metabolic dysfunction and alleviate the symptoms of reproductive system diseases. Overall, the identification of HMGB1 as a key player in disease of the female reproductive system represents a significant breakthrough in our understanding of these conditions and presents exciting opportunities for the development of novel therapies

Comprehensive analysis of the WRKY gene family in Cucumis metuliferus and their expression profile in response to an early stage of root knot nematode infection

Root-knot nematode (RKN) is a major factor that limits the growth and productivity of important Cucumis crops, such as cucumber and melon, which lack RKN-resistance genes in their genome. Cucumis metuliferus is a wild Cucumis species that displays a high degree of RKN-resistance. WRKY transcription factors were involved in plant response to biotic stresses. However, little is known on the function of WRKY genes in response to RKN infection in Cucumis crops. In this study, Cucumis metuliferus 60 WRKY genes (CmWRKY) were identified in the C. metuliferus genome, and their conserved domains were classified into three main groups based on multiple sequence alignment and phylogenetic analysis. Synteny analysis indicated that the WRKY genes were highly conserved in Cucumis crops. Transcriptome data from of C. metuliferus roots inoculated with RKN revealed that 16 CmWRKY genes showed differential expression, of which 13 genes were upregulated and three genes were downregulated, indicating that these CmWRKY genes are important to C. metuliferus response to RKN infection. Two differentially expression CmWRKY genes (CmWRKY10 and CmWRKY28) were selected for further functional analysis. Both CmWRKY genes were localized in nucleus, indicating they may play roles in transcriptional regulation. This study provides a foundation for further research on the function of CmWRKY genes in RKN stress resistance and elucidation of the regulatory mechanism

Experimental Investigation of the Natural Bonding Strength between Stay-In-Place Form and Concrete in FRP-Concrete Decks/Beams

The Fiber Reinforced Polymer (FRP)-concrete hybrid deck/beam is a structural system that combines the durable thin-walled FRP composite profiles and the cost-effective concrete by interfacial shear connections. The interfacial slip can reduce the composite action, thereby causing a degradation of flexural rigidity and capacity. Therefore, using stay-in-place (SIP) forms is a simple way to fully utilize the natural bonding between FRP and concrete, which plays a pivotal role in the structural design of FRP-concrete hybrid decks/beams. This paper presents an experimental study on the natural bonding provided by the SIP forms and the in situ cast concrete. First, four comparative push-out test specimens revealed that the use of SIP forms could improve the ultimate shear capacity of steel bolts by 11.1%. Moreover, it could provide an initial stage with nearly zero slip. The average natural bonding strength of FRP-concrete was evaluated as 0.27 MPa, which agreed well with previous tests in the literature. Second, the beam specimen also confirmed that there was a load response stage with nearly zero slip along the FRP-concrete interface when SIP forms were used as the permanent form. Third, the strain measurements on the steel bolts, FRP profile, and concrete revealed that the failure of the natural bonding was a brittle process. Finally, the flexural response of the FRP-concrete hybrid beam was analytically modeled as three distinct stages, namely the full composite action stage, the slipping stage caused by a natural bonding decrease, and the partial composite action stage

Implementation of an Improved Water Change Tracking (IWCT) Algorithm: Monitoring the Water Changes in Tianjin over 1984–2019 Using Landsat Time-Series Data

Tianjin is the largest open city along the coastline in Northern China, which has several important wetland ecosystems. However, no systematic study has assessed the water body changes over the past few decades for Tianjin, not to mention their response to human activities and climate change. Here, based on the water change tracking (WCT) algorithm, we proposed an improved water change tracking (IWCT) algorithm, which could remove built-up shade noise (account for 0.4%~6.0% of the final water area) and correct omitted water pixels (account for 1.1%~5.1% of the final water area) by taking the time-series data into consideration. The seasonal water product of the Global Surface Water Data (GSWD) was used to provide a comparison with the IWCT results. Significant changes in water bodies of the selected area in Tianjin were revealed from the time-series water maps. The permanent water area of Tianjin decreased 282.5 km2 from 1984 to 2019. Each time after the dried-up period, due to government policies, the land reclamation happened in Tuanbo Birds Nature Reserve (TBNR), and, finally, 12.6 km2 of the lake has been reclaimed. Meanwhile, 488.6 km2 of land has been reclaimed from the sea along the coastal zone in the past 16 years at a speed of 28.74 km2 yr−1 in the Binhai New Area (BHNA). The method developed in this study could be extended to other sensors which have similar band settings with Landsat; the products acquired in this study could provide fundamental reference for the wetland management in Tianjin

Implementation of an Improved Water Change Tracking (IWCT) Algorithm: Monitoring the Water Changes in Tianjin over 1984–2019 Using Landsat Time-Series Data

Tianjin is the largest open city along the coastline in Northern China, which has several important wetland ecosystems. However, no systematic study has assessed the water body changes over the past few decades for Tianjin, not to mention their response to human activities and climate change. Here, based on the water change tracking (WCT) algorithm, we proposed an improved water change tracking (IWCT) algorithm, which could remove built-up shade noise (account for 0.4%~6.0% of the final water area) and correct omitted water pixels (account for 1.1%~5.1% of the final water area) by taking the time-series data into consideration. The seasonal water product of the Global Surface Water Data (GSWD) was used to provide a comparison with the IWCT results. Significant changes in water bodies of the selected area in Tianjin were revealed from the time-series water maps. The permanent water area of Tianjin decreased 282.5 km2 from 1984 to 2019. Each time after the dried-up period, due to government policies, the land reclamation happened in Tuanbo Birds Nature Reserve (TBNR), and, finally, 12.6 km2 of the lake has been reclaimed. Meanwhile, 488.6 km2 of land has been reclaimed from the sea along the coastal zone in the past 16 years at a speed of 28.74 km2 yr&minus;1 in the Binhai New Area (BHNA). The method developed in this study could be extended to other sensors which have similar band settings with Landsat; the products acquired in this study could provide fundamental reference for the wetland management in Tianjin

Experimental Study of Moisture Content Effect on Geotechnical Properties of Solidified Municipal Sludge

Moisture content is an important factor affecting the geotechnical properties of solidified municipal sludge (MMS). Typical municipal sludge in China was chosen to investigate the effects of initial moisture content (defined as w0) on geotechnical properties of MMS solidified by self-developed CERSM solidifying agent. In addition, the microstructure changes of solidified sludge samples in this study were explored with scanning electron microscopy and mercury intrusion porosimetry. Mechanical experiment results showed that the unconfined compressive strength, cohesion, and internal friction angle of the MMS increased with the decrease in initial moisture content, but the permeability coefficient changed oppositely. The permeability coefficient of solidified sludge samples was between 10−8 and 10−10 cm/s. But after the drying-saturated process, the permeability coefficient of MMS can be increased up to 4 times, mainly due to the formation of a considerable number of microconnected pores and microcracks in the process of drying. The research results are of great significance to the safe disposal and utilization of municipal sewage sludge in China