Concomitant Retrograde Coronary Venous Infusion of Basic Fibroblast Growth Factor Enhances Engraftment and Differentiation of Bone Marrow Mesenchymal Stem Cells for Cardiac Repair after Myocardial Infarction.

AIM: Basic fibroblast growth factor (bFGF) increases the migration and viability of bone marrow mesenchymal stem cells (MSCs) in vitro. Retrograde coronary venous infusion can provide both increased regional bFGF concentrations and homogeneous cell dissemination. We determined whether retrograde delivery of bFGF enhances the potency of transplanted MSCs for cardiac repair in a canine infarct model. METHODS AND RESULTS: Under hypoxic conditions, cellular migration was significantly increased in MSCs co-cultured with bFGF compared to vascular endothelial growth factor or insulin-like growth factor, and bFGF promoted MSCs differentiation into a cardiomyocyte phenotype. A canine infarct model was employed by coronary ligation. One week later, animals were subjected to retrograde infusion of combination bFGF (200ng/mL) and MSCs (1×10(8) cells) (n=5), MSCs (1×10(8) cells, n=5), bFGF (200ng/mL, n=5), or placebo (phosphate-buffered saline, n=3). Four weeks after infusion, only the bFGF+MSCs therapy exhibited significantly increased left ventricular ejection fraction (LVEF) by echocardiography (p CONCLUSIONS: Retrograde coronary venous bFGF infusion augments engraftment and differentiation capacity of transplanted MSCs, recovering cardiac function and preventing adverse remodeling. This novel combined treatment and delivery method is a promising strategy for cardiac repair after ischemic injury

Characteristics of clay minerals and their effects on production capacity of the Cretaceous sandstone reservoirs of Songliao Basin, China

Clay minerais in Cretaceous sandstone reservoirs of Songliao Basin are mainly composed of smectite, illite, kaolinite, chlorite and mixed-layer smectite/illite (S/I) and smectite/chlorite (S/Ch). Compositions and content of these clay minerals, and their occurrence patterns of in the sandstone pores are mainly controlled by diagenesis and depositional environment. These characteristics may directly influence the pore textures and the production capacity of the reservoirs. In addition, water sensitivity, acid sensitivity, and microgram migration of in situ clay minerals are the most important potential factors leading to formation damage during processes of well drilling, completion, waterflooding and stimulation.Wang Xingxin. Characteristics of clay minerals and their effects on production capacity of the Cretaceous sandstone reservoirs of Songliao Basin, China. In: Proceedings of the 9th international Clay Conference, Strasbourg, 1989. Vol IV : Clays in sediments. Diagenesis and hydrothermalism. Strasbourg : Institut de Géologie – Université Louis-Pasteur, 1990. pp. 105-114. (Sciences Géologiques. Mémoire, 88

Characteristics of clay minerals and their effects on production capacity of the Cretaceous sandstone reservoirs of Songliao Basin, China

Clay minerais in Cretaceous sandstone reservoirs of Songliao Basin are mainly composed of smectite, illite, kaolinite, chlorite and mixed-layer smectite/illite (S/I) and smectite/chlorite (S/Ch). Compositions and content of these clay minerals, and their occurrence patterns of in the sandstone pores are mainly controlled by diagenesis and depositional environment. These characteristics may directly influence the pore textures and the production capacity of the reservoirs. In addition, water sensitivity, acid sensitivity, and microgram migration of in situ clay minerals are the most important potential factors leading to formation damage during processes of well drilling, completion, waterflooding and stimulation.Wang Xingxin. Characteristics of clay minerals and their effects on production capacity of the Cretaceous sandstone reservoirs of Songliao Basin, China. In: Proceedings of the 9th international Clay Conference, Strasbourg, 1989. Vol IV : Clays in sediments. Diagenesis and hydrothermalism. Strasbourg : Institut de Géologie – Université Louis-Pasteur, 1990. pp. 105-114. (Sciences Géologiques. Mémoire, 88

Silver(I) and Copper(I) Cocatalyzed Tandem Reaction of 2‑Alkynylbenzaldoximes with Aldehydes or Alcohols: Approach to 4‑Carboxylated Isoquinolines

A novel and efficient route for the preparation of 4-carboxylated isoquinolines via a Ag­(I) and Cu­(I) cocatalyzed tandem reaction of 2-alkynylbenzaldoximes with aldehydes or alcohols in moderate to good yields is described. The reaction proceeds smoothly to produce C–N and C–O bonds in a one-pot procedure with structural complexity and molecular diversity

Efficient and secure multi-dimensional geometric range query over encrypted data in cloud

Secure geometric range query, which aims to retrieve data points within a given geometric range from an encrypted dataset in the cloud, attracts more and more attention due to its wide applications. Up to now, several secure geometric range query schemes have been put forward. However, the existing schemes still suffer from various disadvantages, such as they are of low efficiency, cannot support multi-dimensional data and general range query, or even have security flaws. In this paper, we study secure geometric range query on encrypted dataset in cloud. First, we show the security problem of the state-of-the-art scheme by proposing an efficient attack method. Then, we propose a new secure solution for general multi-dimensional range query, which is secure under known-background model, and leverage R-tree index to achieve sub-linear search efficiency. Finally, through theoretical analysis and extensive experiments, we demonstrate the effectiveness and efficiency of our proposed approaches

Locally differentially private distributed algorithms for set intersection and union [Letter]

Privacy-preserving distributed set intersection and union (PPSI, PPSU) have received much attention in recent years because of their wide applications. Most of existing solutions utilize secure multiparty computation protocols (SMCP) to settle the problem, but the SMCP methods are expensive in computation and communication. Even worse, most SMCP methods hardly continue to work if some participants disconnect. In this paper, we consider a distributed model where each data owner has a secret set. Then, we design effective and high-efficiency PPSI/PPSU mechanisms under local differential privacy (LDP), which are suitable for normal sets and multisets. In our proposed schemes, each data owner first sanitizes his own set locally to protect sensitive information. Then, the collector gathers these sanitized datasets from data owners, and estimates the intersection and union from the sanitized sets. Through theoretical analysis, we prove the designed schemes satisfy LDP. Further, we show that our schemes can tolerate the disconnection of some data owners and resist collusion attack of participants. In addition, our schemes have low computation and communication costs. Finally, we evaluate the proposed schemes by conducting extensive experiments, which confirm the effectiveness and efficiency of our schemes

Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4/NF-κB Signaling Pathway

Tec kinase is an important mediator in inflammatory immune response that enhances the activity of neutrophils and macrophages. However, information on its function in lipopolysaccharide- (LPS-) induced acute kidney injury (AKI) is limited. This study is aimed at determining whether Tec kinase was a regulator in AKI. An AKI model in mice was successfully established using intraperitoneal LPS. Results showed that the serum levels of creatinine (Cr), blood urea nitrogen (BUN), and cystatin-C (Cys-C) increased after intraperitoneal LPS injection. Renal tissue sustained significantly severe injury as measured by pathological scores. Pretreatment with LFM-A13 improved the function of the kidney in mice and decreased the renal injury score. Enzyme-linked immunosorbent assay showed that LFM-A13 significantly reduced the release of IL-1β and TNF-α in mice exposed to LPS. LFM-A13 can evidently abrogate the expression of Tec protein, MyD88, TLR4, NF-κB p65, and Tec’s phosphorylated protein as determined by Western blot. Immunohistochemistry analysis revealed that LFM-A13 markedly downregulated the expression of Tec kinase in renal tubular epithelial cells. In vitro, Tec kinase protein was expressed highly in NRK-52E cells after LPS exposure. Tec-siRNA also decreased IL-1β and TNF-α production and obviously abolished phospho-p65 and phospho-IκBα expression in NRK-52E cell stimulated by LPS; however, Tec-siRNA increased the IκBα level. Altogether, these data suggested that Tec kinase can be a modulating protein in AKI through TLR4/NF-κB activation