Differential quadrature solutions to dynamic response of cylindrical shell subjected to thermal shock

The dynamic response of the cylindrical shell subjected to thermal shock is investigated. Based on the classical shell theory, dynamic governing equations of thin shell with the simply supported edges under thermal shock are derived by using Hamilton principle. The temperature field, the thermal axial force and the thermal bending moment are obtained in combination of Laplace transform and series expansion when the internal surface of shell is subjected to thermal shock loading. Considering of the axisymmetric deformation, the transient displacements and thermal stresses of the shell are obtained using the differential quadrature method. The effects of the thermal shock load and the geometrical parameters of the cylindrical shell on the central deflection, the axial displacement, the bending configurations and the transient thermal stresses are analyzed

Carnosol Modulates Th17 Cell Differentiation and Microglial Switch in Experimental Autoimmune Encephalomyelitis

Medicinal plants as a rich pool for developing novel small molecule therapeutic medicine have been used for thousands of years. Carnosol as a bioactive diterpene compound originated from Rosmarinus officinalis (Rosemary) and Salvia officinalis, herbs extensively applied in traditional medicine for the treatment of multiple autoimmune diseases (1). In this study, we investigated the therapeutic effects and molecule mechanism of carnosol in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Carnosol treatment significantly alleviated clinical development in the myelin oligodendrocyte glycoprotein (MOG35–55) peptide-induced EAE model, markedly decreased inflammatory cell infiltration into the central nervous system and reduced demyelination. Further, carnosol inhibited Th17 cell differentiation and signal transducer and activator of transcription 3 phosphorylation, and blocked transcription factor NF-κB nuclear translocation. In the passive-EAE model, carnosol treatment also significantly prevented Th17 cell pathogenicity. Moreover, carnosol exerted its therapeutic effects in the chronic stage of EAE, and, remarkably, switched the phenotypes of infiltrated macrophage/microglia. Taken together, our results show that carnosol has enormous potential for development as a therapeutic agent for autoimmune diseases such as MS

A multi-wavelength observation and investigation of six infrared dark clouds

Context. Infrared dark clouds (IRDCs) are ubiquitous in the Milky Way, yet they play a crucial role in breeding newly-formed stars. Aims. With the aim of further understanding the dynamics, chemistry, and evolution of IRDCs, we carried out multi-wavelength observations on a small sample. Methods. We performed new observations with the IRAM 30 m and CSO 10.4 m telescopes, with tracers ${\rm HCO^+}$, HCN, ${\rm N_2H^+}$, ${\rm C^{18}O}$, DCO$^+$, SiO, and DCN toward six IRDCs G031.97+00.07, G033.69-00.01, G034.43+00.24, G035.39-00.33, G038.95-00.47, and G053.11+00.05. Results. We investigated 44 cores including 37 cores reported in previous work and seven newly-identified cores. Toward the dense cores, we detected 6 DCO$^+$, and 5 DCN lines. Using pixel-by-pixel spectral energy distribution (SED) fits of the $\textit{Herschel}$ 70 to 500 $\mu$m, we obtained dust temperature and column density distributions of the IRDCs. We found that ${\rm N_2H^+}$ emission has a strong correlation with the dust temperature and column density distributions, while ${\rm C^{18}O}$ showed the weakest correlation. It is suggested that ${\rm N_2H^+}$ is indeed a good tracer in very dense conditions, but ${\rm C^{18}O}$ is an unreliable one, as it has a relatively low critical density and is vulnerable to freezing-out onto the surface of cold dust grains. The dynamics within IRDCs are active, with infall, outflow, and collapse; the spectra are abundant especially in deuterium species. Conclusions. We observe many blueshifted and redshifted profiles, respectively, with ${\rm HCO^+}$ and ${\rm C^{18}O}$ toward the same core. This case can be well explained by model "envelope expansion with core collapse (EECC)".Comment: 24 pages, 11 figures, 4 tables. To be published in A&A. The resolutions of the pictures are cut dow

Spatial clustering and common regulatory elements correlate with coordinated gene expression

Many cellular responses to surrounding cues require temporally concerted transcriptional regulation of multiple genes. In prokaryotic cells, a single-input-module motif with one transcription factor regulating multiple target genes can generate coordinated gene expression. In eukaryotic cells, transcriptional activity of a gene is affected by not only transcription factors but also the epigenetic modifications and three-dimensional chromosome structure of the gene. To examine how local gene environment and transcription factor regulation are coupled, we performed a combined analysis of time-course RNA-seq data of TGF-\b{eta} treated MCF10A cells and related epigenomic and Hi-C data. Using Dynamic Regulatory Events Miner (DREM), we clustered differentially expressed genes based on gene expression profiles and associated transcription factors. Genes in each class have similar temporal gene expression patterns and share common transcription factors. Next, we defined a set of linear and radial distribution functions, as used in statistical physics, to measure the distributions of genes within a class both spatially and linearly along the genomic sequence. Remarkably, genes within the same class despite sometimes being separated by tens of million bases (Mb) along genomic sequence show a significantly higher tendency to be spatially close despite sometimes being separated by tens of Mb along the genomic sequence than those belonging to different classes do. Analyses extended to the process of mouse nervous system development arrived at similar conclusions. Future studies will be able to test whether this spatial organization of chromosomes contributes to concerted gene expression.Comment: 30 pages, 9 figures, accepted in PLoS Computational Biolog

Ionic effect on combing of single DNA molecules and observation of their force-induced melting by fluorescence microscopy

Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, we observed that the length of lambda-DNA molecules was extended to about 1.6 times their contour length (unextended length, 16.2 micrometers) by the combing method on hydrophobic polymethylmetacrylate (PMMA) coated surfaces. The effects of sodium and magnesium ions and pH of the DNA solution were investigated. Interestingly, we observed force-induced melting of single DNA molecules.Comment: 12 page

Probing the initial conditions of high-mass star formation -- IV. Gas dynamics and NH2_2D chemistry in high-mass precluster and protocluster clumps

The initial stage of star formation is a complex area study because of its high density and low temperature. Under such conditions, many molecules become depleted from the gas phase by freezing out onto dust grains. However, the deuterated species could remain gaseous and are thus ideal tracers. We investigate the gas dynamics and NH$_2$D chemistry in eight massive pre/protocluster clumps. We present NH$_2$D 1$_{11}$-1$_{01}$ (at 85.926 GHz), NH$_3$ (1, 1) and (2, 2) observations in the eight clumps using the PdBI and the VLA, respectively. We find that the distribution between deuterium fractionation and kinetic temperature shows a number density peak at around $T_{\rm kin}=16.1$ K, and the NH$_2$D cores are mainly located at a temperature range of 13.0 to 22.0 K. We detect seven instances of extremely high deuterium fractionation of $1.0 \leqslant D_{\rm frac} \leqslant 1.41$. We find that the NH$_2$D emission does not appear to coincide exactly with either dust continuum or NH$_3$ peak positions, but often surrounds the star-formation active regions. This suggests that the NH$_{2}$D has been destroyed by the central young stellar object (YSO) due to its heating. The detected NH$_2$D lines are very narrow with a median width of $\rm 0.98\pm0.02 km/s$. The extracted NH$_2$D cores are gravitationally bound ($\alpha_{\rm vir} < 1$), are likely prestellar or starless, and can potentially form intermediate-mass or high-mass stars. Using NH$_3$ (1, 1) as a dynamical tracer, we find very complicated dynamical movement, which can be explained by a combined process with outflow, rotation, convergent flow, collision, large velocity gradient, and rotating toroids. High deuterium fractionation strongly depends on the temperature condition. NH$_2$D is a poor evolutionary indicator of high-mass star formation in evolved stages, but a useful tracer in the starless and prestellar cores.Comment: 27 pages, 25 figures, 6 tables, accepted for publication in A&