Effects of selenoprotein extracts from Cardamine hupingshanensis on growth, selenium metabolism, antioxidant capacity, immunity and intestinal health in largemouth bass Micropterus salmoides

This study aimed to assess the impact of dietary selenoprotein extracts from Cardamine hupingshanensis (SePCH) on the growth, hematological parameters, selenium metabolism, immune responses, antioxidant capacities, inflammatory reactions and intestinal barrier functions in juvenile largemouth bass (Micropterus salmoides). The base diet was supplemented with four different concentrations of SePCH: 0.00, 0.30, 0.60 and 1.20 g/Kg (actual selenium contents: 0.37, 0.59, 0.84 and 1.30 mg/kg). These concentrations were used to formulate four isonitrogenous and isoenergetic diets for juvenile largemouth bass during a 60-day culture period. Adequate dietary SePCH (0.60 and 1.20 g/Kg) significantly increased weight gain and daily growth rate compared to the control groups (0.00 g/Kg). Furthermore, 0.60 and 1.20 g/Kg SePCH significantly enhanced amounts of white blood cells, red blood cells, platelets, lymphocytes and monocytes, and levels of hemoglobin, mean corpuscular volume and mean corpuscular hemoglobin in the hemocytes. In addition, 0.60 and 1.20 g/Kg SePCH increased the mRNA expression levels of selenocysteine lyase, selenophosphate synthase 1, 15 kDa selenoprotein, selenoprotein T2, selenoprotein H, selenoprotein P and selenoprotein K in the fish liver and intestine compared to the controls. Adequate SePCH not only significantly elevated the activities of antioxidant enzymes (Total superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase), the levels of total antioxidant capacity and glutathione, while increased mRNA transcription levels of NF-E2-related factor 2, Cu/Zn-superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase. However, adequate SePCH significantly decreased levels of malondialdehyde and H2O2 and the mRNA expression levels of kelch-like ECH-associated protein 1a and kelch-like ECH-associated protein 1b in the fish liver and intestine compared to the controls. Meanwhile, adequate SePCH markedly enhanced the levels of immune factors (alkaline phosphatase, acid phosphatase, lysozyme, complement component 3, complement component 4 and immunoglobulin M) and innate immune-related genes (lysozyme, hepcidin, liver-expressed antimicrobial peptide 2, complement component 3 and complement component 4) in the fish liver and intestine compared to the controls. Adequate SePCH reduced the levels of pro-inflammatory cytokines (tumour necrosis factor-α, interleukin 8, interleukin 1β and interferon γ), while increasing transforming growth factor β1 levels at both transcriptional and protein levels in the liver and intestine. The mRNA expression levels of mitogen-activated protein kinase 13 (MAPK 13), MAPK14 and nuclear factor kappa B p65 were significantly reduced in the liver and intestine of fish fed with 0.60 and 1.20 g/Kg SePCH compared to the controls. Histological sections also demonstrated that 0.60 and 1.20 g/Kg SePCH significantly increased intestinal villus height and villus width compared to the controls. Furthermore, the mRNA expression levels of tight junction proteins (zonula occludens-1, zonula occludens-3, Claudin-1, Claudin-3, Claudin-5, Claudin-11, Claudin-23 and Claudin-34) and Mucin-17 were significantly upregulated in the intestinal epithelial cells of 0.60 and 1.20 g/Kg SePCH groups compared to the controls. In conclusion, these results found that 0.60 and 1.20 g/Kg dietary SePCH can not only improve growth, hematological parameters, selenium metabolism, antioxidant capacities, enhance immune responses and intestinal functions, but also alleviate inflammatory responses. This information can serve as a useful reference for formulating feeds for largemouth bass

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

A tetrahedron-based endmember selection approach for urban impervious surface mapping.

The pixel purity index (PPI) and two-dimensional (2-D) scatter plots are two popular techniques for endmember extraction in remote sensing spectral mixture analysis, yet both suffer from one major drawback, that is, the selection of a final set of endmembers has to endure a cumbersome process of iterative visual inspection and human intervention, especially when a spectrally-complex urban scene is involved. Within the conceptual framework of a V-H-L-S (vegetation-high albedo-low albedo-soil) model, which is expanded from the classic V-I-S (vegetation-impervious surface-soil) model, a tetrahedron-based endmember selection approach combined with a multi-objective optimization genetic algorithm (MOGA) was designed to identify urban endmembers from multispectral imagery. The tetrahedron defining the enclosing volume of MNF-transformed pixels in a three-dimensional (3-D) space was algorithmically sought, so that the tetrahedral vertices can ideally match the four components of the adopted model. A case study with Landsat Enhanced Thematic Mapper Plus (ETM+) satellite imagery in Shanghai, China was conducted to verify the validity of the method. The method performance was compared with those of the traditional PPI and 2-D scatter plots approaches. The results indicated that the tetrahedron-based endmember selection approach performed better in both accuracy and ease of identification for urban surface endmembers owing to the 3-D visualization analysis and use of the MOGA

Point mutation in CD19 facilitates immune escape of B cell lymphoma from CAR-T cell therapy

Background Tumor relapse due to mutation in CD19 can hinder the efficacy of chimeric antigen receptor (CAR)-T cell therapy. Herein, we focused on lymphoma patients whose B cells exhibited a point mutation in CD19 of B cells after CAR-T cell infusion.Methods The CAR-T and CD19+ B cells from peripheral blood or bone marrow were assessed using flow cytometry. Genome sequencing was conducted to identify the molecular characteristics of CAR-T and CD19+ B cells from pre-relapse and postrelapse samples. CD19 in CARs comprising single chain fragments variable (scFV) antibody with FMC63 or 21D4 was constructed. The cytotoxic efficacy of CAR-T cells was also evaluated via in vitro and in vivo experiments.Results A patient with high-grade B cell lymphoma exhibited complete response, but the lymphoma relapsed in her left breast at 6 months after CD19 CAR (FMC63)-T cell infusion. A mutation was found in exon 3 of CD19 (p.163. R-L) in malignant B cells of the patient. In two lymphoma patients who exhibited resistance to CAR-T cell therapy, a mutation was detected in exon 3 of CD19 (p.174. L-V). Functional analysis revealed that FMC63 CAR-T cells exhibited antitumor ability against wild-type CD19+ cells but were unable to eradicate these two types of mutated CD19+ cells. Interestingly, 21D4 CAR-T cells were potentially capable of eradicating these mutated CD19+ cells and exhibiting high antitumor capacity against CD19+ cells with loss of exon 1, 2, or 3.Conclusions These findings suggest that point mutation can facilitate immune escape from CAR-T cell therapy and that alternative CAR-T cells can effectively eradicate the mutated B cells, providing an individualized therapeutic approach for lymphoma patients showing relapse

Comparison and Intercalibration of Vegetation Indices from Different Sensors for Monitoring Above-Ground Plant Nitrogen Uptake in Winter Wheat

Various sensors have been used to obtain the canopy spectral reflectance for monitoring above-ground plant nitrogen (N) uptake in winter wheat. Comparison and intercalibration of spectral reflectance and vegetation indices derived from different sensors are important for multi-sensor data fusion and utilization. In this study, the spectral reflectance and its derived vegetation indices from three ground-based sensors (ASD Field Spec Pro spectrometer, CropScan MSR 16 and GreenSeeker RT 100) in six winter wheat field experiments were compared. Then, the best sensor (ASD) and its normalized difference vegetation index (NDVI (807, 736)) for estimating above-ground plant N uptake were determined (R2 of 0.885 and RMSE of 1.440 g·N·m−2 for model calibration). In order to better utilize the spectral reflectance from the three sensors, intercalibration models for vegetation indices based on different sensors were developed. The results indicated that the vegetation indices from different sensors could be intercalibrated, which should promote application of data fusion and make monitoring of above-ground plant N uptake more precise and accurate

Comparison and Intercalibration of Vegetation Indices from Different Sensors for Monitoring Above-Ground Plant Nitrogen Uptake in Winter Wheat

Various sensors have been used to obtain the canopy spectral reflectance for monitoring above-ground plant nitrogen (N) uptake in winter wheat. Comparison and intercalibration of spectral reflectance and vegetation indices derived from different sensors are important for multi-sensor data fusion and utilization. In this study, the spectral reflectance and its derived vegetation indices from three ground-based sensors (ASD Field Spec Pro spectrometer, CropScan MSR 16 and GreenSeeker RT 100) in six winter wheat field experiments were compared. Then, the best sensor (ASD) and its normalized difference vegetation index (NDVI (807, 736)) for estimating above-ground plant N uptake were determined (R2 of 0.885 and RMSE of 1.440 g·N·m−2 for model calibration). In order to better utilize the spectral reflectance from the three sensors, intercalibration models for vegetation indices based on different sensors were developed. The results indicated that the vegetation indices from different sensors could be intercalibrated, which should promote application of data fusion and make monitoring of above-ground plant N uptake more precise and accurate

Effect of Surface Roughness on Hydrogen-Induced Blister Behavior in Pure Iron

The effect of surface roughness on hydrogen-induced blister behavior in pure iron was explored using optical microscopy and microcomputerized tomography. Hydrogen was introduced into the samples by electrochemical precharging methods with various hydrogen charging times. It is found that grinded surface exhibits higher roughness and compressive residual stress compared to those of polished surface. With increasing hydrogen charging time, hydrogen-induced blister height, blister width and blister area fraction increase, regardless of the grinded surface and polished surface. Further, hydrogen blister height and width of grinded surface are comparative to polished surface under the same hydrogen charging time. However, the blister area fraction and the number of blister on polished surface are considerably higher than the grinded surface, indicating that polished surface is more susceptible to the formation of blisters than that of grinded surface, both of surface and interior of the samples. This is dominantly rationalized in terms of the suppression effect of hydrogen invasion in grinded surfaces due to their higher residual compressive stress and higher dislocation density

Comprehensive genomic characterisation of the NAC transcription factor family and its response to drought stress in Eucommia ulmoides

The NAC transcription factor family enhances plant adaptation to environmental challenges by participating in signalling pathways triggered by abiotic stressors and hormonal cues. We identified 69 NAC genes in the Eucommia ulmoides genome and renamed them according to their chromosomal distribution. These EuNAC proteins were clustered into 13 sub-families and distributed on 16 chromosomes and 2 scaffolds. The gene structures suggested that the number of exons varied from two to eight among these EuNACs, with a multitude of them containing three exons. Duplicated events resulted in a large gene family; 12 and four pairs of EuNACs were the result of segmental and tandem duplicates, respectively. The drought-stress response pattern of 12 putative EuNACs was observed under drought treatment, revealing that these EuNACs could play crucial roles in mitigating the effects of drought stress responses and serve as promising candidate genes for genetic engineering aimed at enhancing the drought stress tolerance of E. ulmoides. This study provides insight into the evolution, diversity, and characterisation of NAC genes in E. ulmoides and will be helpful for future characterisation of putative EuNACs associated with water deficit

Four endmember fraction maps resulting from LSMA results with endmembers identified from the tetrahedron-based endmember selection approach (fraction values range from 0 to 1, with the lowest values in blue and the highest values in red).

<p>Four endmember fraction maps resulting from LSMA results with endmembers identified from the tetrahedron-based endmember selection approach (fraction values range from 0 to 1, with the lowest values in blue and the highest values in red).</p