Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China

Background: The men having sex with men (MSM) population has become one of the major risk groups for HIV-1 infection in China. However, the epidemiological patterns, function of the env genes, and autologous and heterologous neutralization activity in the same MSM population have not been systematically characterized. Methods: The env gene sequences were obtained by the single genome amplification. The time to the most recent common ancestor was estimated for each genotype using the Bayesian Markov Chain Monte Carlo approach. Coreceptor usage was determined in NP-2 cells. Neutralization was analyzed using Env pseudoviruses in TZM-bl cells. Results: We have obtained 547 full-length env gene sequences by single genome amplification from 30 acute/early HIV-1–infected individuals in the Beijing MSM cohort. Three genotypes (subtype B, CRF01_AE, and CRF07_BC) were identified and 20% of the individuals were infected with multiple transmitted/founder (T/F) viruses. The tight clusters of the MSM sequences regardless of geographic origins indicated nearly exclusive transmission within the MSM population and limited number of introductions. The time to the most recent common ancestor for each genotype was 10–15 years after each was first introduced in China. Disparate preferences for coreceptor usages among 3 genotypes might lead to the changes in percentage of different genotypes in the MSM population over time. The genotype-matched and genotype-mismatched neutralization activity varied among the 3 genotypes. Conclusions: The identification of unique characteristics for transmission, coreceptor usage, neutralization profile, and epidemic patterns of HIV-1 is critical for the better understanding of transmission mechanisms, development of preventive strategies, and evaluation of vaccine efficacy in the MSM population in China

Attosecond Precision Multi-km Laser-Microwave Network

Synchronous laser-microwave networks delivering attosecond timing precision are highly desirable in many advanced applications, such as geodesy, very-long-baseline interferometry, high-precision navigation and multi-telescope arrays. In particular, rapidly expanding photon science facilities like X-ray free-electron lasers and intense laser beamlines require system-wide attosecond-level synchronization of dozens of optical and microwave signals up to kilometer distances. Once equipped with such precision, these facilities will initiate radically new science by shedding light on molecular and atomic processes happening on the attosecond timescale, such as intramolecular charge transfer, Auger processes and their impact on X-ray imaging. Here, we present for the first time a complete synchronous laser-microwave network with attosecond precision, which is achieved through new metrological devices and careful balancing of fiber nonlinearities and fundamental noise contributions. We demonstrate timing stabilization of a 4.7-km fiber network and remote optical-optical synchronization across a 3.5-km fiber link with an overall timing jitter of 580 and 680 attoseconds RMS, respectively, for over 40 hours. Ultimately we realize a complete laser-microwave network with 950-attosecond timing jitter for 18 hours. This work can enable next-generation attosecond photon-science facilities to revolutionize many research fields from structural biology to material science and chemistry to fundamental physics.Comment: 42 pages, 13 figure

A novel configuration model for random graphs with given degree sequence

Recently, random graphs in which vertices are characterized by hidden variables controlling the establishment of edges between pairs of vertices have attracted much attention. Here, we present a specific realization of a class of random network models in which the connection probability between two vertices (i,j) is a specific function of degrees ki and kj. In the framework of the configuration model of random graphs, we find analytical expressions for the degree correlation and clustering as a function of the variance of the desired degree distribution. The expressions obtained are checked by means of numerical simulations. Possible applications of our model are discussed.Comment: 7 pages, 3 figure

A NLO analysis on fragility of dihadron tomography in high energy AAAA collisions

The dihadron spectra in high energy $AA$ collisions are studied within the NLO pQCD parton model with jet quenching taken into account. The high $p_T$ dihadron spectra are found to be contributed not only by jet pairs close and tangential to the surface of the dense matter but also by punching-through jets survived at the center while the single hadron high $p_T$ spectra are only dominated by surface emission. Consequently, the suppression factor of such high-$p_T$ hadron pairs is found to be more sensitive to the initial gluon density than the single hadron suppression factor.Comment: 4 pages, 4 figures, proceedings for the 19th international Conference on ultra-relativistic nucleus-nucleus collisions (QM2006), Shanghai, China, November 14-20, 200

Quasiparticle Scattering Interference in (K,Tl)FexSe2 Superconductors

We model the quasiparticle interference (QPI) pattern in the recently discovered (K,Tl)Fe_xSe2 superconductors. We show in the superconducting state that, due to the absence of hole pockets at the Brillouin zone center, the quasiparticle scattering occurs around the momentum transfer q=(0,0) and (\pm \pi, \pm \pi) between electron pockets located at the zone boundary. More importantly, although both d_{x^2-y^2}-wave and s-wave pairing symmetry lead to nodeless quasiparticle excitations, distinct QPI features are predicted between both types of pairing symmetry. In the presence of a nonmagnetic impurity scattering, the QPI exhibits strongest scattering with q=(\pm \pi, \pm \pi) for the d_{x^2-y^2}-wave pairing symmetry; while the strongest scattering exhibits a ring-like structure centered around both q=(0,0) and (\pm \pi, \pm \pi) for the isotropic s-wave pairing symmetry. A unique QPI pattern has also been predicted due to a local pair-potential-type impurity scattering. The significant contrast in the QPI pattern between the d_{x^2-y^2}-wave and the isotropic s-wave pairing symmetry can be used to probe the pairing symmetry within the Fourier-transform STM technique.Comment: 4+ pages, 3 embedded eps figure

Detecting Extra Dimension by Helium-like Ions

Considering that gravitational force might deviate from Newton's inverse-square law and become much stronger in small scale, we present a method to detect the possible existence of extra dimensions in the ADD model. By making use of an effective variational wave function, we obtain the nonrelativistic ground energy of a helium atom and its isoelectronic sequence. Based on these results, we calculate gravity correction of the ADD model. Our calculation may provide a rough estimation about the magnitude of the corresponding frequencies which could be measured in later experiments.Comment: 8 pages, no figures, accepted by Mod. Phys. Lett.

Isolation and identification of anthocyanin component in the fruits of Acanthopanax Sessiliflorus (Rupr. & Maxim.) Seem. by means of high speed counter current chromatography and evaluation of Its antioxidant activity

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) is one of the most abundant species of genus Acanthopanax. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of A. sessiliflorus. The anthocyanin content in the fresh fruits of A. sessiliflorus was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), 1H- and 13C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH).We found that A. sessiliflorus contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant

The stability of transgene expression and effect of DNA methylation on post transcriptional gene silencing (PTGS) in birch

In this paper, we selected transgenic birch (Betula platyphylla Suk) plants, which included nonsilencing plants, transcriptional silence plants including TP96, TP74, TP73 and the post-transcriptional silence ones (TP67 and TP72). The transcription of the bgt gene in different tissues and organs were significantly different. The transcriptional level of bgt gene in the different tissues and organs was in the following order: leaf > female flower and male flower > branch bark > phloem > root. The transgenic lines were monitored for foreign gene expression for a long-term period of 8 years during their continuous growth under field conditions. GUS protein expression was not reactivated in the transgene silencing lines TP72 and TP67 when cultured in field conditions for long-term period. Meanwhile, no cases of gene silencing were observed again during the study period in the field conditions. Our results suggest that transgene expression in transgenic birch plants appears to be stable under field conditions. The frequencies of methylated cytosines in the code regions of gus gene was studied. Relation of transgene expression and DNA methylation was analysed. The data of restriction enzyme digestion (HpaII and MspI) indicated that DNA methylation resulted in post transcriptional gene silencing (PTGS) in transgenic birch.Key words: Transgenic birch, DNA methylation, gene silencing

The statistical properties of galaxy morphological types in compact groups of Main galaxies from the SDSS Data Release 4

In order to explore the statistical properties of galaxy morphological types in compact groups (CGs), we construct a random group sample which has the same distributions of redshift and number of member galaxies as those of the CG sample. It turns out that the proportion of early-type galaxies in different redshift bins for the CG sample is statistically higher than that for random group sample, and with growing redshift z this kind of difference becomes more significant. This may be due to the existence of interactions and mergers within a significant fraction of SDSS CGs. We also compare statistical results of CGs with those of more compact groups and pairs, but do not observe as large statistical difference as Hickson (1982)'results.Comment: 12 pages, 9 figure