A 55-year-old man with a MIA in the upper lobe of left lung.

<p>Axial (A) and coronal (B) HRCT images show a 13.2mm irregular nodule with spiculated margin (arrow), and intact vessels through lesion with tiny branches (coarse arrow).</p

HRCT morphologic features of lung pre-invasive lesion and MIA.

<p>HRCT morphologic features of lung pre-invasive lesion and MIA.</p

Receiver operating characteristic (ROC) curve for HRCT variables.

<p>The area under ROC curve (AUC) for lesion diameter was 0.760 (A), the AUC for mean HRCT attenuation 0.793 (B), and the AUC for lesion volume 0.898 (C). The optimal cut-off value used for lesion diameter, mean HRCT attenuation and volume to differentiate pre-invasive lesions from MIA was 8.18 mm (sensitivity, 75.0%; specificity, 71.8%; PPV, 44.8%; and NPV, 52.7%), -602 HU (sensitivity, 71.4%; specificity, 76.9%; PPV, 49.1%; and NPV, 50.6%), and 0.33 cm3 (sensitivity, 78.6%; specificity, 87.2%; PPV, 51.9%; and NPV, 48.8%), separately.</p

Nodule contouring process.

<p>(A) The edge of the nodule is traced automatically. (B) The segmented nodule shows different colour in the two-dimensional colour mapping.</p

A 55-year-old man with a MIA in the upper lobe of left lung.

<p>Axial (A) and coronal (B) HRCT images show a 13.2mm irregular nodule with spiculated margin (arrow), and intact vessels through lesion with tiny branches (coarse arrow).</p

Characteristics of pure GGNs (n = 67).

<p>Characteristics of pure GGNs (n = 67).</p

A 52-year-old man with a MIA in the middle lobe of right lung.

<p>Axial (A), sagittal (B) images show an 11.9mm nodule with polygonal shape, irregular and lobulated margin, bubbly lucencies (arrow) and intact vessels distorted (coarse arrow).</p

Can we differentiate minimally invasive adenocarcinoma and non-invasive neoplasms based on high-resolution computed tomography features of pure ground glass nodules? - Fig 5

<p>A 55-year-old woman with an AIS in the upper lobe of left lung. Axial (A) and colour mapping (B) images show a pure GGN. The 6mm nodule shows an oval shape, a smooth border, and vessels through with tiny branches (coarse arrow).</p

Radiologic findings of pure GGN.

<p>A 58-year-old woman with an AAH in the upper lobe of right lung. Axial (A) and coronal (B) HRCT images show a pure GGN with a round shape and a smooth border, and a vessel through pure GGN (type I) (coarse arrow).</p

Table_1_Genomic epidemiology and characterization of Staphylococcus aureus isolates from raw milk in Jiangsu, China: emerging broader host tropism strain clones ST59 and ST398.XLSX

Staphylococcus aureus is highly pathogenic and can cause disease in both humans and domestic animals. The aim of this study was to investigate the genomic epidemiology of S. aureus isolates from raw milk in Jiangsu Province, China, to identify predominant lineages and their associated genomic and phenotypic characteristics. In this study, we identified 117 S. aureus isolates collected from 1,062 samples in Jiangsu Province between 2021 and 2022. Based on whole-genome sequencing (WGS) data from 117 S. aureus isolates, molecular analyses indicated CC1-ST1 (26.50%, 31/117), CC97-ST97 (18.80%, 22/117), CC398-ST398 (10.26%, 12/117), CC8-ST630 (7.69%, 9/117) and CC59-ST59 (2.56%, 3/117) were the major lineages. The prevalence of mecA-positive strains was 11.11%. Four methicillin-resistant S. aureus (MRSA) lineages were found, including MRSA-ST59-t172 (n = 3), OS-MRSA-ST398-t011 (n = 1), MRSA-ST630-t2196 (n = 2) and OS-MRSA-ST630-t2196 (n = 7). Phenotypic resistance to penicillin (30.77%, 36/117), ciprofloxacin (17.09%, 20/117) and erythromycin (15.38%, 18/117) was observed which corresponded with resistance genotypes. All of the isolates could produce biofilms, and 38.46% (45/117) of isolates had invasion rates in mammary epithelial cells (MAC-T) of greater than 1%. Interestingly, most biofilm-producing and invading isolates harbored ebp-icaA-icaB-icaC-icaR-clfA-clfB-fnbA-fnbB-sdrC-sdrD-sdrE-map-can (27.35%, 32/117) and ebp-icaA-icaB-icaC-icaD-icaR-clfA-clfB-fnbA-fnbB-sdrC-sdrD-sdrE-map (33.33%, 39/117) adherence-associated gene patterns and belonged to lineages CC1 and CC97, respectively. Virulence factor assays showed that 47.01% of the isolates contained at least enterotoxin genes. Isolates harboring the immune evasion cluster (IEC) genes (sea, sak, chp, and scn) were predominantly categorized as STs 464, 398, and 59. IEC-positive ST398 and ST59 isolates contained a very high proportion of virulence genes located on prophages, whereas most IEC-negative ST398 clade isolates carried broad-spectrum drug resistance genes. Meanwhile, the IEC-positive ST398 clade showed a close genetic relationship with isolates from the pork supply chain and hospital surgical site infections. MRSA-ST59 strains showed the closest genetic relationship with an isolate from quick-frozen products. High-risk livestock-associated strains ST398 and MRSA-ST59 were detected in raw milk, indicating a potential public health risk of S. aureus transmission between livestock and humans. Our study highlights the necessity for S. aureus surveillance in the dairy industry.</p