Data file from Adult sex ratios and their implications for cooperative breeding in birds

Data used for meta analyse

Additional file 1 of Abnormalities in the SIRT1-SIRT3 axis promote myocardial ischemia-reperfusion injury through ferroptosis caused by silencing the PINK1/Parkin signaling pathway

Supplementary Material

Spectrum-Resolved Dual-Color Electrochemiluminescence Immunoassay for Simultaneous Detection of Two Targets with Nanocrystals as Tags

A spectrum-resolved dual-color electrochemiluminescence (ECL) immunoassay was designed and implemented to simultaneously detect carcinoembryonic antigen (CEA) and alpha fetoprotein (AFP) with CdTe (λ_max = 776 nm) and CdSe (λ_max = 550 nm) nanocrystals (NCs) as ECL tags. The CdTe and CdSe NCs were labeled with respective probe antibodies (Ab₂) of CEA and AFP, respectively, and then immobilized onto the working electrode surface via sandwich-type immunoreactions. Both CdTe and CdSe NCs within the NCs immunocomplexes can be electrochemically reduced and simultaneously give off monochromatic ECL emissions in the near-infrared and greenish regions, respectively, when (NH₄)₂S₂O₈ was used as a cathodic ECL coreactant. The ECL spectra of the two surface-confined NCs were well separated and had no cross energy-transfer interactions, which made the dual-color immunoassay highly selective and sensitive toward respective target analytes. With the proposed ECL biosensor, CEA and AFP were simultaneously detected and quantified with an extremely low detection limit of 1 pg/mL for CEA and 10 fg/mL for AFP, respectively. This work demonstrated the probability of performing multianalyte assays via a spectrum-resolved ECL strategy with improved sensitivity and signal-to-noise ratio as compared to NCs-based fluorescent multianalyte assays

Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

Copper indium sulfide (CuInS₂, CIS) nanocrystals (NCs) are a promising solution to the toxic issue of Cd- and Pb-based NCs. Herein, electrochemiluminescence (ECL) of CIS NCs in aqueous medium is investigated for the first time with l-glutathione and sodium citrate-stabilized water-soluble CIS/ZnS NCs as model. The CIS/ZnS NCs can be oxidized to hole-injected states via electrochemically injecting holes into valence band at 0.55 and 0.94 V (vs Ag/AgCl), respectively. The hole-injected state around 0.94 V can bring out efficient oxidative-reduction ECL with a similar color to Ru­(bpy)₃²⁺ in the presence of tri-<i>n</i>-propylamine (TPrA) and enable CIS/ZnS NCs promising ECL tags with l-glutathione as linker for labeling. The ECL of CIS/ZnS NCs/TPrA can be utilized to determine vascular endothelial growth factor (VEGF) from 0.10 to 1000 pM with the limit of detection at 0.050 pM (S/N = 3). Although the hole-injected state around 0.55 V is generated ahead of oxidation of TPrA and fails to bring out coreactant ECL, annihilation ECL proves that both hole-injected states generated, at 0.55 and 0.94 V, can be involved in electrochemical redox-induced radiative charge transfer by directly stepping CIS/ZnS NCs from electron-injecting potential to hole-injecting potential. CIS/ZnS NCs are promising nontoxic electrochemiluminophores with lowered ECL triggering potential around 0.55 V for less electrochemical interference upon the development of coreactant

Hydrogen Peroxide Involved Anodic Charge Transfer and Electrochemiluminescence of All-Inorganic Halide Perovskite CsPbBr₃ Nanocrystals in an Aqueous Medium

Reactive oxygen species (ROS) involved anodic charge transfer and electrochemiluminescence (ECL) of all-inorganic halide perovskite CsPbBr₃ nanocrystals (NCs) were investigated in an aqueous medium with hydrogen peroxide (H₂O₂) as the model. CsPbBr₃ NCs could be electrochemically oxidized to positively charged states by injecting holes onto the highest occupied molecular orbitals and could be chemically reduced to negatively charged states by injecting electrons onto the lowest unoccupied molecular orbitals by ROS. The charge transfer between CsPbBr₃ NCs of oxidative and reductive states could bring out monochromatic ECL with onset around +0.8 V, maximum emission around 519 nm, and a full width at half-maximum around 20 nm. H₂O₂ could selectively enhance the anodic ECL of CsPbBr₃ NCs, which not only opened a way to design a bioprocess-involved photovoltaic device with CsPbBr₃ NCs but also was promising for color-selective ECL biosensing

Additional file 7 of Intrinsic immune evasion patterns predict temozolomide sensitivity and immunotherapy response in lower-grade gliomas

Additional file 7. R codes

Hua_et_al-Phenology and weather data

1. Raw time series on the first and last sighting of 50 animal taxa collected from 360 meteorological observatories between 1981 and 2009. 2. Weather data from the same sites where phenological data were collected, including records on the daily maximum, minimum, and mean temperature, and daily precipitation. In all, weather data for 349 sites within China were presented