A Petunia homeodomain-leucine zipper protein, PhHD-Zip, plays an important role in flower senescence.

Flower senescence is initiated by developmental and environmental signals, and regulated by gene transcription. A homeodomain-leucine zipper transcription factor, PhHD-Zip, is up-regulated during petunia flower senescence. Virus-induced gene silencing of PhHD-Zip extended flower life by 20% both in unpollinated and pollinated flowers. Silencing PhHD-Zip also dramatically reduced ethylene production and the abundance of transcripts of genes involved in ethylene (ACS, ACO), and ABA (NCED) biosynthesis. Abundance of transcripts of senescence-related genes (SAG12, SAG29) was also dramatically reduced in the silenced flowers. Over-expression of PhHD-Zip accelerated petunia flower senescence. Furthermore, PhHD-Zip transcript abundance in petunia flowers was increased by application of hormones (ethylene, ABA) and abiotic stresses (dehydration, NaCl and cold). Our results suggest that PhHD-Zip plays an important role in regulating petunia flower senescence

Characteristic gene expression in the liver monocyte-macrophage-DC system is associated with the progression of fibrosis in NASH

BackgroundThe monocyte-macrophage-dendritic cell (DC) (MMD) system exerts crucial functions that may modulate fibrogenesis in nonalcoholic steatohepatitis (NASH). In this study, we explored the cell characteristics, distribution and developmental trajectory of the liver MMD system in NASH mice with fibrosis and clarified characteristic genes of the MMD system involved in liver fibrosis progression in NASH mice and patients.MethodsSingle cells in liver tissue samples from NASH and normal mice were quantified using single-cell RNA sequencing (scRNA-seq) analysis. Differentially expressed genes (DEGs) in the MMD system by pseudotime analysis were validated by tyramide signal amplification (TSA)-immunohistochemical staining (IHC) and analyzed by second harmonic generation (SHG)/two-photon excitation fluorescence (TPEF).ResultsCompared with control mice, there were increased numbers of monocytes, Kupffer cells, and DCs in two NASH mouse models. From the transcriptional profiles of these single cells, we identified 8 monocyte subsets (Mono1-Mono8) with different molecular and functional properties. Furthermore, the pseudotime analysis showed that Mono5 and Mono6 were at the beginning of the trajectory path, whereas Mono2, Mono4, Kupffer cells and DCs were at a terminal state. Genes related to liver collagen production were at the late stage of this trajectory path. DEGs analysis revealed that the genes Fmnl1 and Myh9 in the MMD system were gradually upregulated during the trajectory. By TSA-IHC, the Fmnl1 and Myh9 expression levels were increased and associated with collagen production and fibrosis stage in NASH mice and patients.ConclusionsOur transcriptome data provide a novel landscape of the MMD system that is involved in advanced NASH disease status. Fmnl1 and Myh9 expression in the MMD system was associated with the progression of NASH fibrosis

Evaluation Method on Energy-Efficient Retrofitting of Wooden Walls of Chinese Traditional Dwelling—A Case Study of Rendetang in Jinhua

Traditional wooden dwellings, which are widely distributed with enormous stocks in China, are of great historical and have obvious cultural value. The walls of such buildings are generally subjected to poor thermal insulation performance, which not only reduces residential thermal comfort but also increases building cooling and heating energy consumption. In addition, buildings of this type have been subjected to all kinds of problems, such as the lack of measures for improving thermal comfort and the shortage of special funds. Consequently, it is very challenging to reutilize Chinese traditional dwellings, many of which are abandoned and even collapsed. All of the above have become major difficulties encountered in the traditional dwelling heritage protection. Hence, investigating the energy-efficient retrofitting strategies for traditional dwellings and giving economical evaluation methods are two keys to solving the reutilization problem of traditional dwellings. Against this background, a set of second-level evaluation methods for the energy-efficient retrofitting of Chinese traditional dwellings are proposed in this research, including the survey on retrofitted dwellings, the retrofitted dwelling modeling and energy consumption analysis, the definition of wall retrofitting scheme, the first-level evaluation of dwelling retrofitting, the second-level evaluation of dwelling retrofitting, and the screening of the wall retrofitting scheme. The first-level evaluation, which took energy efficiency as a reference index, could evaluate the energy conservation effect before and after dwelling retrofitting. With the payback period as the reference index, the second-level evaluation could assess the overall economic efficiency of dwelling wall retrofitting. An appropriate dwelling wall retrofitting scheme could be screened by integrating first-level and second-level evaluation indexes. Then, this scheme was applied to evaluate the wooden wall retrofitting scheme of a typical traditional dwelling in Yapan Village, Zhejiang Province, China. It was discovered through a comparative analysis that if used to reconstruct dwellings in Zhejiang and other places, the combined materials of XPS board and wood-bamboo could not only effectively improve the energy efficiency but also has good economic efficiency. Meanwhile, problems such as the condensation of wooden walls and their construction thickness could be solved by controlling the material thickness. The above research is of guiding significance for the energy-efficient retrofitting of traditional wooden dwellings in the hot-summer and cold-winter zone of China, and moreover, it can provide reference for the energy-efficient retrofitting of traditional wooden dwellings in other climate zones of China

Recent Advances in Adipose Tissue Dysfunction and Its Role in the Pathogenesis of Non-Alcoholic Fatty Liver Disease

Obesity is a serious ongoing health problem that significantly increases the incidence of nonalcoholic fatty liver disease (NAFLD). During obesity, adipose tissue dysfunction is obvious and characterized by increased fat deposition (adiposity) and chronic low-grade inflammation. The latter has been implicated to critically promote the development and progression of NAFLD, whose advanced form non-alcoholic steatohepatitis (NASH) is considered one of the most common causes of terminal liver diseases. This review summarizes the current knowledge on obesity-related adipose dysfunction and its roles in the pathogenesis of hepatic steatosis and inflammation, as well as liver fibrosis. A better understanding of the crosstalk between adipose tissue and liver under obesity is essential for the development of new and improved preventive and/or therapeutic approaches for managing NAFLD

Research on the Cultural Tracing of the Patriarchal Clan System of Traditional Buildings in the Eastern Zhejiang Province, China, Based on Space Syntax: The Case Study of Huzhai in Shaoxing

The patriarchal clan system is an important prerequisite for the formation and development of Chinese traditional culture. The spatial layout and space usage of traditional buildings are intimately related to patriarchal culture. Thus, analyzing the spatial layout and usage is an effective way to trace the culture of traditional buildings. In this study, a typical traditional building named Huzhai in eastern Zhejiang was examined as an example. The spatial layout characteristics of Huzhai and different space usage relations corresponding to different users under the influence of the patriarchal clan system were investigated through “all lines” analysis in space syntax. In this process, traditional ritual activities were considered crucial for tracing the culture of traditional buildings in the eastern Zhejiang province. The results demonstrate that spatial layout and the usage of traditional buildings in the eastern Zhejiang province have led to “class” distinctions under the influence of patriarchal culture. The sacrificial activities of families further emphasize the class distinctions of building space. The differences in building space usage among different classes reflect the unequal distribution of social resources in China’s traditional feudal society. These differences reflect the inequality of space mastership and control among different classes that are a result of the unequal distribution of social resources in China

Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

Foxtail millet has been traditionally considered to possess gastroprotective effects, but studies evaluating its use as a treatment for gastric ulcers are lacking. Here, we assessed the antiulcer effects of foxtail millet protein hydrolysate (FPH) and explored its mechanism by using blocking agents. In a mouse model of ethanol-induced gastric ulcers, pretreatment with FPH reduced the ulcerative lesion index, downregulated the expression of inflammatory cytokines in the gastric tissue, increased the activity of antioxidant enzymes, and improved the oxidative status. FPH increased constitutive the activity of nitric oxide synthase (cNOS), NO levels, and mucin expression in gastric mucosa, and inhibited the activation of the ET-1/PI3K/Akt pathway. In a mouse model of pyloric ligation-induced gastric ulcers, FPH inhibited gastric acid secretion and decreased the activity of gastric protease. Pretreatment of mice with the sulfhydryl blocker NEM and the NO synthesis inhibitor L-NAME abolished the gastroprotective effect of FPH, but not the KATP channel blocker glibenclamide and the PGE2 synthesis blocker indomethacin. Among the peptides identified in FPH, 10 peptides were predicted to have regulatory effects on the gastric mucosa, and the key sequences were GP and PG. The results confirmed the gastroprotective effect of FPH and revealed that its mechanism was through the regulation of gastric mucosal mucus and NO synthesis. This study supports the health effects of a millet-enriched diet and provides a basis for millet protein as a functional food to improve gastric ulcers and its related oxidative stress

Longevity of unpollinated and pollinated WT, <i>PhHD-Zip</i> silenced white flowers, and vector control flowers (<i>CHS</i> silenced white, PhHD-Zip/CHS purple).

<p>Longevity of attached flowers. Means±SD for 30 flowers (10 from each of three replicate plants). Different letters indicate significant differences using Duncan’s test, p<0.05.</p

Longevity of attached flowers from WT plants and from plants over-expressing <i>PhHD-Zip</i> (35S::<i>PhHD-Zip</i> lines: #3 and #7).

<p>Different letters denote significant differences using Duncan’s test, p<0.05.</p

Effect of drought on longevity of WT, <i>CHS</i> silenced control and the <i>PhHD-Zip</i> silenced petunia flowers.

<p>Means±SD for 15 detached flowers (5 from each of 3 replicate plants) and 30 attached flowers (10 from each of 3 replicate plants. Different letters indicate significant differences using Duncan’s test, p<0.05.</p