OR-041 Infection of Different Altitudes on College Students’ Body Shape

Objective Considering that guizhou is located in a plateau region with various gradient altitude,and we have never seen any relevant report on the study of different altitudes influence the students'physical fitness at home or abroad. the aim of this research is the author runs a normal physical fitness test on native 774 non-PE major students who all comes from different altitudes 774, so as to provide reference standard for promoting the health of the students group. Methods literature data, fitness testing, statistic, and logic analysis. Results 1、There was no obvious consistency between the height change of students in different atitudes indicating that the influence of different altitude environments on the height of students was not obvious.2、Most of the students on the plateau show the thickness of the skinfold is the thickest , while the thickness of the skinfold of students on the plain and the subplateau is different and irregular. 3. There was no significant difference in the chest circumference of the four groups of students at three altitudes, indicating that the environment at different altitudes had no significant influence on the development of the respiratory organs and chest muscles of the students. 4. The waist circumference of the three groups showed no obvious pattern, indicating that the environment at different altitude had no significant influence on the waist circumference. 5. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range. 6. The BMI of all the students in the three places is within the range of 18.5 ~ 22.9, that is, all the students in the three places are in the normal range. Conclusions altitude has no significant effect on students'height, chest circumference, waist circumference and BMI. The thickness of skinfold of students on plateau is thicker than that of plain and subplateau students. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range

Analysis of 116 cases of rectal cancer treated by transanal local excision

BACKGROUND: The purpose of this research was to evaluate the therapeutic effects and prognostic factors of transanal local excision (TAE) for rectal cancer. METHODS: We retrospectively analyzed 116 cases that underwent TAE for rectal cancer from 1995 to 2008. A Cox regression analysis was used to analyze prognostic factors. RESULTS: The survival times for the patients were from 14 to 160.5 months (median time, 58.5 months). The 5-year and 10-year overall survival rates were 72% and 53%, respectively. In all 16 cases experienced local recurrence (13.8%). Pathological type, recurrence or metastasis, and depth of infiltration (T stage) were the prognostic factors according to the univariate analysis, and the latter two were independent factors affecting patient prognosis. For patients with T1 stage who underwent adjuvant radiotherapy, there was no local recurrence; for those in T2 stage, the local recurrence rate was 14.6%. In addition, there was no difference between the patients who received radiotherapy and those who did not (T1: P = 0.260, T2: P = 0.262 for survival rate and T1: P = 0.480, T2: P = 0.560 for recurrence). CONCLUSIONS: The result of TAE for rectal cancer is satisfactory for T1 stage tumors, but it is not suitable for T2 stage tumors

Use of Ambr15 as a high throughput model to speed up perfusion bioprocess development

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Ethyl 2-benzyl-1-propyl-1H-indole-3-carboxyl­ate

In the title compound, C21H23NO2, the dihedral angle between the indole ring system and the benzyl ring is 75.92 (9)°. The crystal packing is controlled by C—H⋯O and C—H⋯π inter­actions

Cellular trafficking of Sn-2 phosphatidylcholine prodrugs studied with fluorescence lifetime imaging and super-resolution microscopy

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Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin

Using novel media formulations, it has been demonstrated that human placenta and umbilical cord blood-derived CD34+ cells can be expanded and differentiated into erythroid cells with high efficiency. However, obtaining mature and functional erythrocytes from the immature cell cultures with high purity and in an efficient manner remains a significant challenge. A distinguishing feature of a reticulocyte and maturing erythrocyte is the increasing concentration of hemoglobin and decreasing cell volume that results in increased cell magnetophoretic mobility (MM) when exposed to high magnetic fields and gradients, under anoxic conditions. Taking advantage of these initial observations, we studied a noninvasive (label-free) magnetic separation and analysis process to enrich and identify cultured functional erythrocytes. In addition to the magnetic cell separation and cell motion analysis in the magnetic field, the cell cultures were characterized for cell sedimentation rate, cell volume distributions using differential interference microscopy, immunophenotyping (glycophorin A), hemoglobin concentration and shear-induced deformability (elongation index, EI, by ektacytometry) to test for mature erythrocyte attributes. A commercial, packed column high-gradient magnetic separator (HGMS) was used for magnetic separation. The magnetically enriched fraction comprised 80% of the maturing cells (predominantly reticulocytes) that showed near 70% overlap of EI with the reference cord blood-derived RBC and over 50% overlap with the adult donor RBCs. The results demonstrate feasibility of label-free magnetic enrichment of erythrocyte fraction of CD34+ progenitor-derived cultures based on the presence of paramagnetic hemoglobin in the maturing erythrocytes. © 2012 Jin et al

Perceived Discrimination and Life Satisfaction of Elderly Chinese People: The Chain Mediating Effects of National Identity and Sense of Community

In China, aging is becoming an increasingly serious issue, and the Chinese government are paying more attention to the life satisfaction of the elderly. Nevertheless, in their daily lives, the elderly are often discriminated against, which may have a negative impact on their life satisfaction. To enable a better understanding of these relationships, we discuss the factors affecting the macro-system (national identity) and micro-system (sense of community) of the elderly. Three hundred and ninety-one elderly people (60–101 years old; 121 males, 270 females) from three communities in the Anhui and Shandong provinces of China participated in our study. Each participant completed the appropriate questionnaires, including: perceived discrimination measure, national identity questionnaire, sense of community questionnaire, and life satisfaction questionnaire. The results of structural equation modeling revealed that perceived discrimination negatively influenced life satisfaction through national identity and community. Perceived discrimination was found to negatively predict national identity, suggesting that perceived discrimination brings a negative influence to national identity within Chinese culture. The relationship between perceived discrimination and life satisfaction was partially mediated by the chain of national identity and sense of community. The size of the total mediation effect was 32.17%. The relationship between perceived discrimination and life satisfaction, when mediated by national identity or sense of community, was not significant. This suggests that the application of the rejection-identification model to the elderly in China may produce different results. The limitations and the implications of our study were considered in discussion

PO-072 Study on immune function monitoring during altitude training for adolescent athletes

Objective Monitoring the changes of the body's immune function during altitude training is of great significance for understanding the athlete's physical function and judging the degree of fatigue. In this paper, the immune function of adolescent athletes in sports training in different altitudes was monitored, and the effects of different altitude training on immunoglobulins and T lymphocytes and their subgroups in adolescent athletes were discussed. Methods Male adolescent middle and long distance runners were divided into two groups according to training performance, 2500m group (10 people, age: 14.8±1.4 years old, height: 163.6±7.3cm, weight: 49.5±6.0kg, training period: 1.2±0.6 years) and 1800m group (10 people, age: 15.7±1.7 years old, height: 164.7±8.8cm, weight: 49.2±6.1kg, training period: 1.3±0.8 years) volunteered to participate in the 2-stage training (3 weeks plateau and 3 weeks plain). The change of CD3+CD4、CD3+CD8+CD4+/CD8+IgAIgGIgM were tested every week.  Results The result showed that: (1)The 2500m group had shown statistical differences of CD3+CD4+CD3+CD8+ at different times, and CD4+/CD8+ had no changes. The 1800m group had not shown statistical differences of CD3+CD4+CD4+/CD8+ at different times. Compared with the base value, the CD3+CD8+ was significantly increased (P<0.01). There was no statistical differences of CD3+CD4CD3+CD8+CD4+/CD8+ at different times between two groups. (2)The 2500m group had not shown significant changes of IgA. Three weeks altitude training following with three week plain training the IgM and IgG showed a downward trend; and compared with altitude period, the IgM and IgG was lower in plain(P<0.05). The 1800m group had not shown statistical differences of IgAIgMIgG at different times. There was no statistical differences of IgAIgMIgG at different times between two groups. Conclusions The immunity function of adolescent athletes is more affected during high altitude training, suggesting that the adolescent athletes in the pursuit of high altitude training should pay attention to the monitoring and regulation of immunity function. CD3+CD8+ is more sensitive to hypoxia which can be used as a sensitive indicator and has significant meaning in monitoring altitude training

Involvement of CYP2E1 in the Course of Brain Edema Induced by Subacute Poisoning With 1,2-Dichloroethane in Mice

This study was designed to explore the role of cytochrome P4502E1 (CYP2E1) expression in the course of brain edema induced by subacute poisoning with 1,2-dichloroethane (1,2-DCE). Mice were randomly divided into five groups: the control group, the 1,2-DCE poisoned group, and the low-, medium- and high-dose diallyl sulfide (DAS) intervention groups. The present study found that CYP2E1 expression levels in the brains of the 1,2-DCE-poisoned group were upregulated transcriptionally; in contrast, the levels were suppressed by DAS pretreatment in the intervention groups. In addition, the expression levels of both Nrf2 and HO-1 were also upregulated transcriptionally in the brains of the 1,2-DCE-poisoned group, while they were suppressed dose-dependently in the intervention groups. Moreover, compared with the control group, MDA levels and water contents in the brains of the 1,2-DCE-poisoned group increased, whereas NPSH levels and tight junction (TJ) protein levels decreased significantly. Conversely, compared with the 1,2-DCE- poisoned group, MDA levels and water contents in the brains of the intervention groups decreased, and NPSH levels and TJ protein levels increased significantly. Furthermore, pathological changes of brain edema observed in the 1,2-DCE-poisoned group were markedly improved in the intervention groups. Collectively, our results suggested that CYP2E1 expression could be transcriptionally upregulated in 1,2-DCE-poisoned mice, which might enhance 1,2-DCE metabolism in vivo, and induce oxidative damage and TJ disruption in the brain, ultimately leading to brain edema